Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,863,165	T→C	R277R (CGT→CGC)	ygbK →	FliA‑regulated DUF1537 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,863,165	0	T	C	100.0%	66.9 / NA	19	R277R (CGT→CGC)	ygbK	FliA‑regulated DUF1537 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/6);  total (13/6)

AAACGGTAACCAGGCTCGCAAAGCAGGGCGTCCGCTCGCTGGGCGCGGCGTAGTGCTCTCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGA  >  NC_000913/2863042‑2863290                                                                                                                            |                                                                                                                              aaaCGGTAACCAGGCTCGCAAAGCAGGGCGTCCGCTCGCTGGGCGCGGCGTAGTGCTCTCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGCGAAGTTGATGTGGCa                                                                                                                <  2:248576/139‑1 (MQ=255)              gCTCGCAAAGCAGGGCGTCCGCTCGCTGGGCGCGGCGTAGTGCTCTCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGCGAAGTTGATGTGGCACGCTGCCTCTCaa                                                                                                   >  1:313491/1‑139 (MQ=255)                           ggCGTCCGCTCGCTGGGCGCGGCGTAGTGCTCTCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGCGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGc                                                                                      <  2:589586/139‑1 (MQ=255)                                  gctcgctGGGCGCGGCGTAGTGCTCTCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGCGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTAt                                                                               >  1:578501/1‑139 (MQ=255)                                     cgctGGGCGCGGCGTAGTGCTCTCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGCGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGca                                                                            >  2:1005804/1‑139 (MQ=255)                                       ctGGGCGCGGCGTAGTGCTCTCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGCGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGcaca                                                                          <  1:1005804/139‑1 (MQ=255)                                                           tcCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGCGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTc                                                      >  1:536027/1‑139 (MQ=255)                                                                ttCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGCGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGaa                                                                      <  2:442985/118‑1 (MQ=255)                                                                ttCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGCGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGaa                                                                      >  1:442985/1‑118 (MQ=255)                                                                  cATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGCGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCa                                               >  2:878319/1‑139 (MQ=255)                                                                    tGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGCGAAg                                                                                                                           >  1:694918/1‑61 (MQ=255)                                                                    tGCTCTCAAATGACCAACCGCCAGGTAGCACAGTACCGTCAAATTGCACCAGCCCGCGAAg                                                                                                                           <  2:694918/61‑1 (MQ=255)                                                                       tctcAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGCGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGaaa                                          >  1:42477/1‑139 (MQ=255)                                                                              tGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGCGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACt                                   <  2:663567/139‑1 (MQ=255)                                                                                   aaCCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGCGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGGAAGTGTACTTGCTc                              >  1:580467/1‑139 (MQ=255)                                                                                   aaCCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGCGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACTTGCTc                              >  1:320305/1‑139 (MQ=255)                                                                                                     tACCGTCAAATTGCACCAGCCCGCGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACTTGCTGCACTGGTTTTTGCCACCg            >  2:752574/1‑139 (MQ=255)                                                                                                        cGTCAAATTGCACCAGCCCGCGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCa         >  2:595512/1‑139 (MQ=255)                                                                                                               ttGCACCAGCCCGCGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGa  >  1:414242/1‑139 (MQ=255)                                                                                                                            |                                                                                                                              AAACGGTAACCAGGCTCGCAAAGCAGGGCGTCCGCTCGCTGGGCGCGGCGTAGTGCTCTCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGA  >  NC_000913/2863042‑2863290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑