Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,895,747	C→T	E11E (GAG→GAA)	ygcQ ←	putative flavoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,895,747	0	C	T	100.0%	120.0 / NA	36	E11E (GAG→GAA)	ygcQ	putative flavoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/18);  total (18/18)

CGTTCGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCT  >  NC_000913/2895615‑2895878                                                                                                                                     |                                                                                                                                    cGTTCGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGa                                                                                                                               >  2:90307/1‑139 (MQ=255)   ttCGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTAAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGAtt                                                                                                                             >  1:358176/1‑139 (MQ=255)            aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTcac                                                                                                                    >  1:238762/1‑139 (MQ=255)                   caccaACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCg                                                                                                             <  2:318883/139‑1 (MQ=255)                    accaACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGa                                                                                                            >  2:981531/1‑139 (MQ=255)                        aCGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATAtt                                                                                                        >  2:1028194/1‑139 (MQ=255)                         cGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTc                                                                                                       <  2:320594/139‑1 (MQ=255)                            aTCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTAAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATg                                                                                                    <  2:358176/139‑1 (MQ=255)                             tCCAGCACCTGTTCCGCCACTGCAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATgg                                                                                                   <  2:658007/139‑1 (MQ=255)                                              cACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGACGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCtttacgcca                                                                                  <  1:981531/139‑7 (MQ=255)                                                cTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTaaa                                                                                <  2:31592/139‑1 (MQ=255)                                                cTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTaaa                                                                                <  2:238762/139‑1 (MQ=255)                                                     ggCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTc                                                                           >  2:239812/1‑139 (MQ=255)                                                          aGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCt                                                                                          >  1:627633/1‑119 (MQ=255)                                                          aGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCt                                                                                          <  2:627633/119‑1 (MQ=255)                                                          aGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGcc                                                                      >  2:758054/1‑139 (MQ=255)                                                                    tGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCATAAATAGTCCTGCCATAGCTTTTg                                                            <  1:758054/139‑1 (MQ=255)                                                                     gCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTc                                                                           >  1:875521/1‑123 (MQ=255)                                                                     gCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTc                                                                           <  2:875521/123‑1 (MQ=255)                                                                          tGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGAt                                                                                                                              <  1:146877/67‑1 (MQ=255)                                                                          tGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGAt                                                                                                                              >  2:146877/1‑67 (MQ=255)                                                                                       aaCCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCt                                                                                          <  2:669133/90‑1 (MQ=255)                                                                                       aaCCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCt                                                                                          >  1:669133/1‑90 (MQ=255)                                                                                        aCCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGttt                                        <  1:898701/139‑1 (MQ=255)                                                                                        aCCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGttt                                        <  1:1095141/139‑1 (MQ=255)                                                                                            cTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGc                                                                                               >  2:1032282/1‑80 (MQ=255)                                                                                            cTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGc                                                                                               <  1:1032282/80‑1 (MQ=255)                                                                                            cTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGccc                                    >  2:572446/1‑139 (MQ=255)                                                                                            cTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGccc                                    >  1:1072668/1‑139 (MQ=255)                                                                                             tGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCa                                                                                                      <  1:723305/72‑1 (MQ=255)                                                                                             tGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCa                                                                                                      >  2:723305/1‑72 (MQ=255)                                                                                                       cgccgcCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCt                                                                                          <  2:1094675/74‑1 (MQ=255)                                                                                                       cgccgcCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCt                                                                                          >  1:1094675/1‑74 (MQ=255)                                                                                                                    cTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGcgtcg            <  1:572446/139‑1 (MQ=255)                                                                                                                            tGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCCCGTCGTtgctctg    >  1:726651/1‑139 (MQ=255)                                                                                                                              cGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTtgctctgct  >  1:325584/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                    CGTTCGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCT  >  NC_000913/2895615‑2895878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑