| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,451,938 | G→A | R117C (CGT→TGT) | rplD ← | 50S ribosomal subunit protein L4 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,451,938 | 0 | G | A | 100.0% | 98.2 / NA | 29 | R117C (CGT→TGT) | rplD | 50S ribosomal subunit protein L4 |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/15); total (14/15) | |||||||||||
GCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGTCTTTCAGTTTCTGTGCCAGCAGCTTAGTTTTCGGCGCTTCTACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGA > NC_000913/3451803‑3452069 | gcgcAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGTCTTTCAGTTTCTGTGCCAGCAGCTTAGTTTTCGGCGCTTCTACAGAGAACTTCTCGACAACGATCAGACAATc > 2:257735/1‑139 (MQ=255) cAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGTCTTTCAGTTTCTGTGCCAGCAGCTTAGTTTTCGGCGCTTCTACAGAGAACTTCTCGACAACGATCAGACAATCCTg < 1:1112213/139‑1 (MQ=255) aGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGTCTTTCAGTTTCTGTGCCAGCAGCTTAGTTTTCGGCGCTTCTACAGAGAACTTCTCGACAACGATCAGACAATCCTGACGTACCAGtt < 1:257735/139‑1 (MQ=255) gatCAGCACATCTTCCAGAGCCATGTCTTTCAGTTTCTGTGCCAGCAGCTTAGTTTTCGGCGCTTCTACAGAGAACTTCTCGACAACGATCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGcgccg < 1:921791/139‑1 (MQ=255) cacaTCTTCCAGAGCCATGTCTTTCAGTTTCTGTGCCAGCAGCTTAGTTTTCGGCGCTTCTACAGAGAACTTCTCGACAACGATCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTAc > 2:981983/1‑139 (MQ=255) aTCTTCCAGAGCCATGTCTTTCAGTTTCTGTGCCAGCAGCTTAGTTTTCGGCGCTTCTACAGAGAACTTCTCGACAACGATCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACAtc < 1:146441/139‑1 (MQ=255) aTGTCTTTCAGTTTCTGTGCCAGCAGCTTAGTTTTCGGCGCTTCTACAGAGAACTTCTCGACAACGATCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACtt > 2:573910/1‑139 (MQ=255) tGTCTTTCAGTTTCTGTGCCAGCAGCTTAGTTTTCGGCGCTTCTACAGAGAACTTCTCGACAACGATCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACttt > 1:160568/1‑139 (MQ=255) tGTCTTTCAGTTTCTGTGCCAGCAGCTTAGTTTTCGGCGCTTCTACAGAGAACTTCTCGACAACGATCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACttt > 2:607542/1‑139 (MQ=255) gTCTTTCAGTTTCTGTGCCAGCAGCTTAGTTTTCGGCGCTTCTACAGAGAACTTCTCGACAACGATCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACtttt > 2:799753/1‑139 (MQ=255) tCTTTCAGTTTCTGTGCCAGCAGCTTAGTTTTCGGCGCTTCTACAGAGAACTTCTCGACAACGATCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACttttt < 1:799753/139‑1 (MQ=255) ttCAGTTTCTGTGCCAGCAGCTTAGTTTTCGGCGCTTCTACAGAGAACTTCTCGACAACGATCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGAc > 2:136723/1‑139 (MQ=255) cAGTTTCTGTGCCAGCAGCTTAGTTTTCGGCGCTTCTACAGAGAACTTCTCGACAACGATCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACtg > 2:616435/1‑139 (MQ=255) tCTGTGCCAGCAGCTTAGTTTTCGGCGCTTCTACAGAGAACTTCTCGACAACGATCAGACAATCCTGACGTACCAGTTCGGACa < 1:97129/84‑1 (MQ=255) tCTGTGCCAGCAGCTTAGTTTTCGGCGCTTCTACAGAGAACTTCTCGACAACGATCAGACAATCCTGACGTACCAGTTCGGACa > 2:97129/1‑84 (MQ=255) ccAGCAGCTTAGTTTTCGGCGCTTCTACAGAGAACTTCTCGACAACGATCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCgg < 2:160568/139‑1 (MQ=255) aGTTTTCGGCGCTTCTACAGAGAACTTCTCGACAACGATCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAgcagca < 1:136723/139‑1 (MQ=255) tACAGAGAACTTCTCGACAACGATCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGccacca < 1:611422/139‑1 (MQ=255) aaCTTCTCGACAACGATCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGc > 2:290284/1‑127 (MQ=255) aaCTTCTCGACAACGATCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGc < 1:290284/127‑1 (MQ=255) aaCTTCTCGACAACGATCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGcc > 1:3295/1‑139 (MQ=255) ctcGACAACGATCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACttttt > 2:282135/1‑86 (MQ=255) ctcGACAACGATCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACttttt < 1:282135/86‑1 (MQ=255) gACAACGATCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGGAGCAAAGGTCACGCCACCAGAACGCCAGATCggg < 2:976549/139‑1 (MQ=255) gACAACGATCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCggg < 2:1070280/139‑1 (MQ=255) gACAACGATCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCggg > 2:25832/1‑139 (MQ=255) aTCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGa < 2:551914/139‑1 (MQ=255) aTCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGa < 2:973335/139‑1 (MQ=255) aTCAGACAATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGa > 1:551914/1‑139 (MQ=255) | GCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGTCTTTCAGTTTCTGTGCCAGCAGCTTAGTTTTCGGCGCTTCTACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGA > NC_000913/3451803‑3452069 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |