| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,648,024 | T→C | intergenic (‑236/‑39) | dinQ ← / → agrA | UV‑inducible membrane toxin, DinQ‑AgrB type I toxin‑antitoxin system/inactive antisense sRNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,648,024 | 0 | T | C | 98.6% | 256.2 / ‑4.5 | 71 | intergenic (‑236/‑39) | dinQ/agrA | UV‑inducible membrane toxin, DinQ‑AgrB type I toxin‑antitoxin system/inactive antisense sRNA |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (38/32); total (38/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-01 | |||||||||||
GCCAGAGCATCAGTTAACGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGA > NC_000913/3647888‑3648159 | gCCAGAGCATCAGTTAACGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGccc > 1:89048/1‑139 (MQ=255) gTTAACGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGttt < 2:229903/139‑1 (MQ=255) gTTAACGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGttt < 2:258645/139‑1 (MQ=255) gTTAACGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGttt < 1:293235/139‑1 (MQ=255) gTTAACGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGttt < 1:625646/139‑1 (MQ=255) aaCGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAg > 1:1029664/1‑139 (MQ=255) cGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTa < 2:1004793/139‑1 (MQ=255) cGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTa < 1:964432/139‑1 (MQ=255) cGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTa < 1:688770/139‑1 (MQ=255) accaccCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGt > 1:662086/1‑139 (MQ=255) caccCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCa > 2:904025/1‑121 (MQ=255) caccCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCa < 1:904025/121‑1 (MQ=255) caccCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTAt > 2:822890/1‑134 (MQ=255) caccCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTAt < 1:822890/134‑1 (MQ=255) caccCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTAt > 1:963962/1‑139 (MQ=255) cTTCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTg < 1:488317/139‑1 (MQ=255) cTTCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTg < 1:527072/139‑1 (MQ=255) ttCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGa < 1:937446/139‑1 (MQ=255) ttCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGa < 2:802105/139‑1 (MQ=255) tCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGAt < 2:431145/139‑1 (MQ=255) gACCGGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGa > 1:66542/1‑135 (MQ=255) gACCGGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGa < 2:66542/135‑1 (MQ=255) aGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGt < 1:655776/136‑1 (MQ=255) aGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGt > 2:655776/1‑136 (MQ=255) gACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAgat < 2:571783/139‑1 (MQ=255) tttGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAgatgat < 1:356201/139‑1 (MQ=255) agAAAACGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGCGCTAACTTGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAATTTCAGTATGGTATTAGCATTGATGCGTTAGATGATgg > 1:381945/2‑139 (MQ=255) gAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGc < 1:940519/139‑1 (MQ=255) tGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCaga > 2:532970/1‑139 (MQ=255) aaaaTGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTa > 2:476377/1‑80 (MQ=255) aaaaTGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTa < 1:476377/80‑1 (MQ=255) aaaaTGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAga > 2:238994/1‑104 (MQ=255) aaaaTGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAga < 1:238994/104‑1 (MQ=255) aaaaTGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATgg < 1:704563/110‑1 (MQ=255) aaaaTGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATgg > 2:704563/1‑110 (MQ=255) aaaaTGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACt > 1:1028760/1‑139 (MQ=255) aaTGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCa > 2:478344/1‑139 (MQ=255) agCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTa > 1:842174/1‑68 (MQ=255) agCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTa < 2:842174/68‑1 (MQ=255) aCTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATgg < 2:613059/93‑1 (MQ=255) aCTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATgg > 1:613059/1‑93 (MQ=255) aCTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTaaa > 2:1006024/1‑139 (MQ=255) ggATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATg > 1:769754/1‑77 (MQ=255) ggATAATCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATg < 2:769754/77‑1 (MQ=255) tCATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACg > 2:754137/1‑139 (MQ=255) cATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAgatg < 1:577894/79‑1 (MQ=255) cATACAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAgatg > 2:577894/1‑79 (MQ=255) cAGTACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAg > 2:68300/1‑139 (MQ=255) tACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCg > 2:981152/1‑139 (MQ=255) tACATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCg > 1:613444/1‑139 (MQ=255) cATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGt > 1:1112159/1‑139 (MQ=255) cATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGt > 1:502643/1‑139 (MQ=255) cATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGt > 2:1026913/1‑139 (MQ=255) cATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGt > 1:837748/1‑139 (MQ=255) cATGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTGAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGt > 2:601681/1‑139 (MQ=255) aTGCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGtt < 1:1006024/139‑1 (MQ=255) gCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGccac < 1:768584/95‑1 (MQ=255) gCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGccac > 2:768584/1‑95 (MQ=255) gCAGGTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGtttt > 1:229478/1‑139 (MQ=255) ggTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGttttttt > 2:537370/1‑139 (MQ=255) ggTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGttttttt > 1:1018408/1‑139 (MQ=255) gTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTa > 1:782641/1‑139 (MQ=255) gTTATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTa > 1:1093042/1‑139 (MQ=255) ttATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTAc > 1:698711/1‑139 (MQ=255) ttATAAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTAc > 2:237026/1‑139 (MQ=255) ttATAAAACCAGCACGCCCTTGCAATAGTTACAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTAc > 1:115952/1‑139 (MQ=255) ataAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAgat > 1:381136/1‑59 (MQ=255) ataAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAgat < 2:381136/59‑1 (MQ=255) taAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATc < 2:1093042/139‑1 (MQ=255) taAAACCAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATc < 2:502643/139‑1 (MQ=255) ccAGCACGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGAt < 2:782641/139‑1 (MQ=255) aCGCCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGa < 2:837748/139‑1 (MQ=255) | GCCAGAGCATCAGTTAACGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGA > NC_000913/3647888‑3648159 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |