Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,004	A→G	G114G (GGT→GGC)	tdh ←	L‑threonine 3‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,004	0	A	G	100.0%	231.8 / NA	62	G114G (GGT→GGC)	tdh	L‑threonine 3‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/33);  total (29/33)

ACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAAT  >  NC_000913/3790868‑3791132                                                                                                                                         |                                                                                                                                 aCGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGcc                                                                                                                                >  2:390501/1‑139 (MQ=255)   gACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCaa                                                                                                                              <  1:1082835/139‑1 (MQ=255)        cgcgGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCt                                                                                                                         >  2:1090633/1‑139 (MQ=255)        cgcgGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCt                                                                                                                         >  1:281778/1‑139 (MQ=255)         gcgGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTa                                                                                                                        <  1:1090633/139‑1 (MQ=255)                    ggCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGcc                                                                                                                                >  2:541114/1‑120 (MQ=255)                    ggCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGcc                                                                                                                                <  1:541114/120‑1 (MQ=255)                     gCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTAGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGc                                                                                                            >  2:584801/1‑139 (MQ=255)                      cGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGca                                                                                                           <  1:946665/139‑1 (MQ=255)                         tACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCGTATCGTGTTGCGGCACaa                                                                                                        >  2:1056130/1‑139 (MQ=255)                         tACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACaa                                                                                                        <  1:237556/139‑1 (MQ=255)                          aCCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTGGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACaaa                                                                                                       <  1:802713/139‑1 (MQ=255)                            cGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATg                                                                                                     >  1:661536/1‑139 (MQ=255)                                gggATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTa                                                                                                 >  1:570168/1‑139 (MQ=255)                                   aTCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGa                                                                                              >  2:485315/1‑139 (MQ=255)                                       aaaaTTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGaccac                                                                                          <  2:661536/139‑1 (MQ=255)                                           ttGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACg                                                                                      >  1:8175/1‑139 (MQ=255)                                           ttGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACg                                                                                      >  2:162509/1‑139 (MQ=255)                                                      tCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGc                                                                           >  1:526567/1‑139 (MQ=255)                                                        aTCGGGACTATTGCCGGGGATTTTGAAGGCGTTGACTGCCGGAACCACTGGCTATACGGCACAGCGACCCGGGCGATTAACGCCCACGCCTATCCTGTCGCTTTATAAATGGGTACGACCACCACGAGAGTTGCGGCaa                                                                         <  1:455802/139‑1 (MQ=255)                                                         tCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAAt                                                                        <  1:485315/139‑1 (MQ=255)                                                               atatTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCa                                                                                                                               <  1:1076277/78‑1 (MQ=255)                                                               atatTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCa                                                                                                                               >  2:1076277/1‑78 (MQ=255)                                                               atatTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACcac                                                                  <  1:130461/139‑1 (MQ=255)                                                                 atTGTGGGGGATTTTGAAGGCGTTGAATGCCGGGATGATGAGTGATTCGGCAAAGCAGCACGGGCGATTAACGCGAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGAGCACAg                                                                <  1:219125/139‑1 (MQ=255)                                                                 atTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAg                                                                <  2:281778/139‑1 (MQ=255)                                                                     tCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGa                                                            >  2:668371/1‑139 (MQ=255)                                                                       ggggATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGcc                                                                                                                                >  2:424857/1‑69 (MQ=255)                                                                       ggggATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGcc                                                                                                                                <  1:424857/69‑1 (MQ=255)                                                                       ggggATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGata                                                          >  2:225099/1‑139 (MQ=255)                                                                           aTTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGc                                                      >  2:612592/1‑139 (MQ=255)                                                                               tGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCaa                                                                         <  2:263611/116‑1 (MQ=255)                                                                               tGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCaa                                                                         >  1:263611/1‑116 (MQ=255)                                                                                       ttGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGaaa                                          >  2:971971/1‑139 (MQ=255)                                                                                           aTGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAAcgcg                                      <  2:570168/139‑1 (MQ=255)                                                                                               cGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAg                                                                >  1:647677/1‑109 (MQ=255)                                                                                               cGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAg                                                                <  2:647677/109‑1 (MQ=255)                                                                                                  gATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCg                               <  1:162509/139‑1 (MQ=255)                                                                                                        cAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTg                         <  1:971971/139‑1 (MQ=255)                                                                                                          gATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGaa                       <  1:584801/139‑1 (MQ=255)                                                                                                             atTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGcc                    >  1:415838/1‑139 (MQ=255)                                                                                                               tCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGt                                                                                 <  1:234837/76‑1 (MQ=255)                                                                                                               tCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGt                                                                                 >  2:234837/1‑76 (MQ=255)                                                                                                               tCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCtt                  >  1:555193/1‑139 (MQ=255)                                                                                                               tCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCtt                  >  2:475633/1‑139 (MQ=255)                                                                                                                cGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGca                                                                                                           <  2:451994/49‑1 (MQ=255)                                                                                                                cGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGca                                                                                                           >  1:451994/1‑49 (MQ=255)                                                                                                                cGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCttt                 <  1:612592/139‑1 (MQ=255)                                                                                                                cGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCttt                 <  1:475633/139‑1 (MQ=255)                                                                                                                 ggCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGaaa                                          >  1:160191/1‑113 (MQ=255)                                                                                                                 ggCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGaaa                                          <  2:160191/113‑1 (MQ=255)                                                                                                                      agcagcCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTc           <  1:390501/139‑1 (MQ=255)                                                                                                                      agcagcCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTc           <  1:331194/139‑1 (MQ=255)                                                                                                                      agcagcCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTc           <  1:300119/139‑1 (MQ=255)                                                                                                                          gcCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGa       <  2:859949/139‑1 (MQ=255)                                                                                                                           cccGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGAc      >  2:906995/1‑139 (MQ=255)                                                                                                                           cccGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGAc      >  2:1089395/1‑139 (MQ=255)                                                                                                                             cGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCa    <  2:892343/139‑1 (MQ=255)                                                                                                                               ggCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAAt  <  1:1089395/139‑1 (MQ=255)                                                                                                                               ggCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAAt  <  1:327768/139‑1 (MQ=255)                                                                                                                                   aTTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGAc      <  2:584436/131‑1 (MQ=255)                                                                                                                                   aTTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGAc      >  1:584436/1‑131 (MQ=255)                                                                                                                                         |                                                                                                                                 ACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAAT  >  NC_000913/3790868‑3791132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑