Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,830,989 T→C V178A (GTT→GCT)  yicH → putative inner membrane‑anchored periplasmic AsmA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,830,9890TC100.0% 99.2 / NA 27V178A (GTT→GCT) yicHputative inner membrane‑anchored periplasmic AsmA family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/14);  total (13/14)

CGAATAGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGTTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGGGTGCCGACATCGCCCGCGGGACATTAACCGGAAACGCGCAGCGTAACGCCGACGGCAGCTGGCAAGT  >  NC_000913/3830857‑3831121
                                                                                                                                    |                                                                                                                                    
cGAATAGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGc                                                                                                                                >  1:340305/1‑139 (MQ=255)
cGAATAGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGc                                                                                                                                <  1:464065/139‑1 (MQ=255)
  aaTAGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGcca                                                                                                                              <  1:138795/139‑1 (MQ=255)
  aaTAGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGcca                                                                                                                              >  2:531019/1‑139 (MQ=255)
   aTAGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGccac                                                                                                                             <  2:340305/139‑1 (MQ=255)
                aCTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGAtt                                                                                                                >  2:18168/1‑139 (MQ=255)
                    aGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAg                                                                                                            <  2:767212/139‑1 (MQ=255)
                    aGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAg                                                                                                            >  1:967104/1‑139 (MQ=255)
                      cgcgcAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGc                                                                                                          <  1:412003/139‑1 (MQ=255)
                                        cggcgTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGc                                                                                                          >  1:1062941/1‑121 (MQ=255)
                                        cggcgTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGc                                                                                                          <  2:1062941/121‑1 (MQ=255)
                                         ggcgTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTAc                                                                                                                     >  1:338337/1‑109 (MQ=255)
                                         ggcgTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTAc                                                                                                                     <  2:338337/109‑1 (MQ=255)
                                         ggcgTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAgg                                                                                                           <  1:578747/118‑1 (MQ=255)
                                         ggcgTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAgg                                                                                                           >  2:578747/1‑118 (MQ=255)
                                                          aCCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACg                                                                                  >  1:123281/1‑127 (MQ=255)
                                                          aCCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACg                                                                                  <  2:123281/127‑1 (MQ=255)
                                                                    ggTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGc                                                                                                                                >  1:866789/1‑71 (MQ=255)
                                                                    ggTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGc                                                                                                                                <  2:866789/71‑1 (MQ=255)
                                                                                ggTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGa                                                                                                              >  1:930359/1‑77 (MQ=255)
                                                                                ggTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGa                                                                                                              <  2:930359/77‑1 (MQ=255)
                                                                                            cAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCa                                                                                                         >  1:611051/1‑70 (MQ=255)
                                                                                            cAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCa                                                                                                         <  2:611051/70‑1 (MQ=255)
                                                                                            cAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGTGTGCCGACATCGCCCGCGGGACATTAACCGGaa                                    >  2:155249/1‑139 (MQ=255)
                                                                                                                aTCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGGGTGCCGACATCGCCCGCGGGACATTAACCGGAAACGCGCAGCGTAACGCCGAc                <  1:74086/139‑1 (MQ=255)
                                                                                                                aTCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGGGTGCCGACATCGCCCGCGGGACATTAACCGGAAACGCGCAGCGTAACGCCGAc                <  1:18168/139‑1 (MQ=255)
                                                                                                                              atgatgCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGGGTGCCGACATCGCCCGCGGGACATTAACCGGAAACGCGCAGCGTAACGCCGACGGCAGCTGGCAAGt  >  2:579695/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                    
CGAATAGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGTTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGGGTGCCGACATCGCCCGCGGGACATTAACCGGAAACGCGCAGCGTAACGCCGACGGCAGCTGGCAAGT  >  NC_000913/3830857‑3831121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: