Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,079,101	A→G	intergenic (+663/‑196)	yiiD → / → yiiE	GNAT family putative N‑acetyltransferase/CopG family putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,079,101	0	A	G	100.0%	194.2 / NA	51	intergenic (+663/‑196)	yiiD/yiiE	GNAT family putative N‑acetyltransferase/CopG family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/25);  total (26/25)

GGTGGCAGTACTGACCGGTCTGTGATGAAAGATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCACCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGCAAAAACAAAACAACATGCAAAAATCCGCTTTTTTTAAATGAT  >  NC_000913/4078966‑4079234                                                                                                                                        |                                                                                                                                      ggtggCAGTACTGACCGGTCTGTGATGAAAGATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGccc                                                                                                                                    <  1:1118199/139‑1 (MQ=255)            tGACCGGTCTGTGATGAAAGATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGCCCATAATTCATcc                                                                                                                         <  1:672132/139‑1 (MQ=255)                 ggTCTGTGATGAAAGATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGc                                                                                                                    <  1:258537/139‑1 (MQ=255)                       tgatgaAAGATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAAc                                                                                                              <  1:1054230/139‑1 (MQ=255)                       tgatgaAAGATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAAc                                                                                                              <  2:99247/139‑1 (MQ=255)                           gaAAGATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTaaa                                                                                                          <  2:97588/139‑1 (MQ=255)                              aGATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCa                                                                                                       >  2:802917/1‑139 (MQ=255)                                 ttAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCAttt                                                                                                    >  1:471383/1‑139 (MQ=255)                                   aaTTTCACCTTATATCCTGATGCTGTTAGGATTCTGACGTTTTTCGTCTACACCATTCAGAGTATGCAAATAAATATCTCTTAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCAtttgt                                                                                                  <  1:799608/139‑3 (MQ=255)                                       tCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGt                                                                                              <  2:1089880/139‑1 (MQ=255)                                                aTCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGa                                                                                     <  2:275014/139‑1 (MQ=255)                                                 tCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGaa                                                                                    <  2:471383/139‑1 (MQ=255)                                                     ggTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCAttttt                                                                                                  <  1:700881/121‑1 (MQ=255)                                                     ggTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCAttttt                                                                                                  >  2:700881/1‑121 (MQ=255)                                                      gTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAaacaac                                                                               >  1:21094/1‑139 (MQ=255)                                                         cTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGc                                                                            >  1:403735/1‑139 (MQ=255)                                                           gTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCaa                                                                          <  2:802336/139‑1 (MQ=255)                                                           gTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCaa                                                                          <  1:554177/139‑1 (MQ=255)                                                           gTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATACAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCaa                                                                          <  1:802917/139‑1 (MQ=255)                                                                                     cacCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGcc                                                >  1:546450/1‑139 (MQ=255)                                                                                      acCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGa                                                                                     <  1:93947/101‑1 (MQ=255)                                                                                      acCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGa                                                                                     >  2:93947/1‑101 (MQ=255)                                                                                          ttCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGCa                                           >  2:482990/1‑139 (MQ=255)                                                                                               aTTATGCAAATAAATATCTCTGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGCAaaaac                                      >  2:499631/1‑139 (MQ=255)                                                                                                         taaataTCTCTGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGCAAAAACAAAACAACAt                            >  1:305568/1‑139 (MQ=255)                                                                                                                ctctGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGCAAAAACAAAACAACATGCaaaaa                     >  2:447969/1‑139 (MQ=255)                                                                                                                 tctGAATATAATATATTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGCAAAAACAAAACAACATGCAAAAAt                    >  2:44620/1‑139 (MQ=255)                                                                                                                          aatataTTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGCAAAAACAAAACAACATGCAAAAATCCGCttttt           >  1:179108/1‑139 (MQ=255)                                                                                                                               atTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTTTGGAAAACGCCTCGCaaaa                                        <  2:745878/105‑1 (MQ=255)                                                                                                                               atTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGCaaaa                                        >  1:745878/1‑105 (MQ=255)                                                                                                                               atTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGCaaa                                         >  2:500432/1‑104 (MQ=255)                                                                                                                               atTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGCaaa                                         <  1:500432/104‑1 (MQ=255)                                                                                                                               atTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGCAAAAACAAAACAACATGCAAAAATCCGCTTTTTTTaaa      >  1:1110760/1‑139 (MQ=255)                                                                                                                               atTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGCAAAAACAAAACAACATGCAAAAATCCGCTTTTTTTaaa      >  1:799298/1‑139 (MQ=255)                                                                                                                               atTCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGCAAAAACAAAACAACATGCAAAAATCCGCTTTTTTTaaa      <  1:447969/139‑1 (MQ=255)                                                                                                                                 tCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCaa                                                                          <  1:751914/69‑1 (MQ=255)                                                                                                                                 tCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCaa                                                                          >  2:751914/1‑69 (MQ=255)                                                                                                                                 tCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGCAAAAACAAaacaac                              <  1:814750/113‑1 (MQ=255)                                                                                                                                 tCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGCAAAAACAAaacaac                              >  2:814750/1‑113 (MQ=255)                                                                                                                                 tCATAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGCAAAAACAAAACAACATGCAAAAATCCGCTTTTTTTAAATg    >  2:608029/1‑139 (MQ=255)                                                                                                                                   aTAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCaa                                                                          <  2:240520/67‑1 (MQ=255)                                                                                                                                   aTAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCaa                                                                          >  1:240520/1‑67 (MQ=255)                                                                                                                                   aTAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGc                                            <  1:93617/97‑1 (MQ=255)                                                                                                                                   aTAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGc                                            >  2:93617/1‑97 (MQ=255)                                                                                                                                   aTAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGCAAAAACAAAACAACATGCAAAAATCCGCTTTTTTTAAATGAt  >  1:536425/1‑139 (MQ=255)                                                                                                                                    tAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGtaa                                                                                            >  2:343804/1‑48 (MQ=255)                                                                                                                                    tAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGtaa                                                                                            <  1:343804/48‑1 (MQ=255)                                                                                                                                    tAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAaac                                                                                  <  1:536172/58‑1 (MQ=255)                                                                                                                                    tAGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAaac                                                                                  >  2:536172/1‑58 (MQ=255)                                                                                                                                     aGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGCAAAAACAAAACAACATGCAAAAATCCg                 <  2:1118895/122‑1 (MQ=255)                                                                                                                                     aGCGCCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGCAAAAACAAAACAACATGCAAAAATCCg                 >  1:1118895/1‑122 (MQ=255)                                                                                                                                        |                                                                                                                                      GGTGGCAGTACTGACCGGTCTGTGATGAAAGATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATATAATATATTCATAGCACCCATAATTCATCCTGGGCGATAACTAAACCATTTTTGAGTAATAAATGAAACAACTGCAACACTCTTATTATTTCTGGAAAACGCCTCGCAAAAACAAAACAACATGCAAAAATCCGCTTTTTTTAAATGAT  >  NC_000913/4078966‑4079234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑