Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,291,336	C→T	G287G (GGC→GGT)	nrfD →	formate‑dependent nitrite reductase, membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,291,336	0	C	T	100.0%	70.3 / NA	22	G287G (GGC→GGT)	nrfD	formate‑dependent nitrite reductase, membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/13);  total (9/13)

CGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTAT  >  NC_000913/4291217‑4291461                                                                                                                        |                                                                                                                              cGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGTATTCCTGCCGTGCTggcgg                                                                                                            <  2:590141/139‑1 (MQ=255)     cgcTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGTATTCCTGCCGTGCTGGCGGCgtg                                                                                                        <  1:83693/139‑1 (MQ=255)                    tAGGTGGCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGTATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGt                                                                                         >  1:744872/1‑139 (MQ=255)                                      tgtggtgGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGTATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTgcg                                                                       <  1:442152/138‑1 (MQ=255)                                      cGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGTATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTgcg                                                                       <  2:1101783/139‑1 (MQ=255)                                             gtTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGTATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTc                                                                <  1:72437/139‑1 (MQ=255)                                                cTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGTATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCAtt                                                             >  1:204628/1‑139 (MQ=255)                                                         tgtCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGTATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGcc                                                    <  2:204628/139‑1 (MQ=255)                                                                  gctggggctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGTATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTc                                                                <  2:906928/118‑1 (MQ=255)                                                                  gctggggctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGTATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTc                                                                >  1:906928/1‑118 (MQ=255)                                                                      gggctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGTATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACgg                                       <  1:276509/139‑1 (MQ=255)                                                                           gATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGTAGTTCCGGTATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGCTGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCg                                  <  1:791900/139‑1 (MQ=255)                                                                            aTTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGTATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCgtgt                                                                                 >  1:574136/1‑91 (MQ=255)                                                                            aTTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGTATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCgtgt                                                                                 <  2:574136/91‑1 (MQ=255)                                                                            aTTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGTATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGAc                                         <  1:818666/131‑1 (MQ=255)                                                                            aTTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGTATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGAc                                         >  2:818666/1‑131 (MQ=255)                                                                                                      ggTCAATCGCAGTTCCGGTATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTgg        >  2:252407/1‑138 (MQ=255)                                                                                                      ggTCAATCGCAGTTCCGGTATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTgg        <  1:252407/138‑1 (MQ=255)                                                                                                        tCAATCGCAGTTCCGGTATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACg     >  2:1055866/1‑139 (MQ=255)                                                                                                           aTCGCAGTTCCGGTATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTAt  >  1:727559/1‑139 (MQ=255)                                                                                                                  ttCCGGTATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTc           <  2:21058/123‑1 (MQ=255)                                                                                                                  ttCCGGTATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTc           >  1:21058/1‑123 (MQ=255)                                                                                                                        |                                                                                                                              CGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTAT  >  NC_000913/4291217‑4291461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑