Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,293,898	A→G	Y119C (TAC→TGC)	nrfG →	heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,293,898	0	A	G	100.0%	116.4 / NA	31	Y119C (TAC→TGC)	nrfG	heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/18);  total (13/18)

GAACAGTGGGCGTTACTGGGCGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCA  >  NC_000913/4293762‑4294027                                                                                                                                         |                                                                                                                                  gAACAGTGGGCGTTACTGGGCGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGcc                                                                                                                                 <  1:507667/139‑1 (MQ=255)      gTGGGCGTTACTGGGCGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGgcc                                                                                                                            <  1:7755/139‑1 (MQ=255)         ggCGTTACTGGGCGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGgccagc                                                                                                                         <  2:843743/139‑1 (MQ=255)                 tGGGCGAGTACTATCTGTGGCAAAACGATTACAGTAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCatat                                                                                                                 <  2:718970/139‑1 (MQ=255)                   ggCGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGa                                                                                                               <  2:877413/139‑1 (MQ=255)                              tCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGATTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACt                                                                                                    <  2:1119027/139‑1 (MQ=255)                                   ggCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACt                                                                                                    >  2:354019/1‑134 (MQ=255)                                   ggCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACt                                                                                                    <  1:354019/134‑1 (MQ=255)                                                                       gTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGa                                                           <  2:265147/139‑1 (MQ=255)                                                                             cGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCAc                                                     <  2:1007679/139‑1 (MQ=255)                                                                                 gCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGcc                                                 >  1:1039276/1‑139 (MQ=255)                                                                                        cGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGAtgc                                          >  2:176441/1‑139 (MQ=255)                                                                                        cGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGAtgc                                          >  2:119190/1‑139 (MQ=255)                                                                                        cGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAACCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGAtgc                                          >  2:769184/1‑139 (MQ=255)                                                                                          tggtgAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGAtgctg                                        <  2:1042397/139‑1 (MQ=255)                                                                                            gtgAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATgctgct                                      >  2:21677/1‑139 (MQ=255)                                                                                                gaACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATCATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCt                                  >  1:659933/1‑139 (MQ=255)                                                                                                 aaCGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTcgcg                                                                         <  2:814070/99‑1 (MQ=255)                                                                                                 aaCGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTcgcg                                                                         >  1:814070/1‑99 (MQ=255)                                                                                                 aaCGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGa                                                                    <  1:578610/104‑1 (MQ=255)                                                                                                 aaCGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGa                                                                    >  2:578610/1‑104 (MQ=255)                                                                                                     cTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAACCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGa                             <  1:769184/139‑1 (MQ=255)                                                                                                              aTGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGa                                                                                          >  2:69606/1‑69 (MQ=255)                                                                                                              aTGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGa                                                                                          <  1:69606/69‑1 (MQ=255)                                                                                                              aTGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTAt                    >  2:100958/1‑139 (MQ=255)                                                                                                                  ggcgCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTcg                                                                           <  2:713748/80‑1 (MQ=255)                                                                                                                  ggcgCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTcg                                                                           >  1:713748/1‑80 (MQ=255)                                                                                                                       tGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGaa           >  1:282522/1‑139 (MQ=255)                                                                                                                              ggTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTAcgcg    <  2:33664/139‑1 (MQ=255)                                                                                                                                tGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCa  <  1:517435/139‑1 (MQ=255)                                                                                                                                tGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCa  <  2:282522/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  GAACAGTGGGCGTTACTGGGCGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCA  >  NC_000913/4293762‑4294027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑