Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 337,217 T→C V147A (GTC→GCC)  yahF → putative NAD(P)‑binding succinyl‑CoA synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913337,2170TC100.0% 137.9 / NA 38V147A (GTC→GCC) yahFputative NAD(P)‑binding succinyl‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/21);  total (17/21)

GCCACTATTGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGTCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACA  >  NC_000913/337081‑337351
                                                                                                                                        |                                                                                                                                      
gCCACTATTGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGcct                                                                                                                                      <  1:488005/139‑1 (MQ=255)
        tGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGaa                                                                                                                              <  2:994395/139‑1 (MQ=255)
            aGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATg                                                                                                                          >  2:258501/1‑139 (MQ=255)
              cgcCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGcct                                                                                                                                      <  2:467406/125‑1 (MQ=255)
              cgcCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGaa                                                                                                                        >  2:1096211/1‑139 (MQ=255)
                           atCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGc                                                                                                           >  2:327197/1‑139 (MQ=255)
                             cccGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCaa                                                                                                         >  2:258206/1‑139 (MQ=255)
                                ggAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTg                                                                                                      <  2:1037660/139‑1 (MQ=255)
                                        aCCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGCTCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCaa                                                                                                         <  2:362112/128‑1 (MQ=255)
                                        aCCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCaa                                                                                                         >  1:362112/1‑128 (MQ=255)
                                           tGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCa                                                                                                              <  1:758015/120‑1 (MQ=255)
                                           tGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCa                                                                                                              >  2:758015/1‑120 (MQ=255)
                                              cGGTGATTTCTGTCAACGATCTGTTTGCCGCTCGCGAAGCGCGCCAGGCGCTGCAAAACGATCTCAACGTGATGCTCGTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGGAACTGGCCCACGAAAAAgg                                                                                        >  1:801258/1‑139 (MQ=255)
                                                       cGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATACTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCtgat                                                                               >  2:614952/1‑139 (MQ=255)
                                                        ggTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCtgatg                                                                              >  1:553886/1‑139 (MQ=255)
                                                        ggTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCtgatg                                                                              >  2:769798/1‑139 (MQ=255)
                                                             aCGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATggg                                                                         >  1:593580/1‑139 (MQ=255)
                                                                          cGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGgct                                                                                     <  1:478774/114‑1 (MQ=255)
                                                                          cGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGgct                                                                                     >  2:478774/1‑114 (MQ=255)
                                                                              cgcgAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACgg                                                        <  2:553886/139‑1 (MQ=255)
                                                                              cgcgAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACgg                                                        <  1:801785/139‑1 (MQ=255)
                                                                               gcgAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGc                                                       >  1:150221/1‑139 (MQ=255)
                                                                                   aGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGAtt                                                   <  1:755298/139‑1 (MQ=255)
                                                                                       cgTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCa                                               >  2:186790/1‑139 (MQ=255)
                                                                                                   cAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACggcgcggcgc                                   <  1:796714/139‑1 (MQ=255)
                                                                                                   cAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTTCAACGGCGCGGCGct                                  <  1:211677/139‑1 (MQ=255)
                                                                                                    aaaaCGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGct                                  <  2:150221/139‑1 (MQ=255)
                                                                                                    aaaaCGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGct                                  <  1:1096211/139‑1 (MQ=255)
                                                                                                                      tGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGct                                  >  2:435093/1‑121 (MQ=255)
                                                                                                                      tGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGct                                  <  1:435093/121‑1 (MQ=255)
                                                                                                                         tGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGgc                                                                                                                   >  1:214763/1‑37 (MQ=255)
                                                                                                                         tGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGgc                                                                                                                   <  2:214763/37‑1 (MQ=255)
                                                                                                                                cGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAggg                                                                                       >  1:72253/1‑58 (MQ=255)
                                                                                                                                cGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAggg                                                                                       <  2:72253/58‑1 (MQ=255)
                                                                                                                                cGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGc      <  1:628561/139‑1 (MQ=255)
                                                                                                                                cGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGc      <  2:801258/139‑1 (MQ=255)
                                                                                                                                  aTAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCaa    <  1:649909/139‑1 (MQ=255)
                                                                                                                                  aTAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCaa    <  1:327197/139‑1 (MQ=255)
                                                                                                                                  aTAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCaa    <  1:258501/139‑1 (MQ=255)
                                                                                                                                    aaCGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACa  <  2:1028825/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
GCCACTATTGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGTCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACA  >  NC_000913/337081‑337351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: