Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 337,217 | T→C | V147A (GTC→GCC) | yahF → | putative NAD(P)‑binding succinyl‑CoA synthase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 337,217 | 0 | T | C | 100.0% | 137.9 / NA | 38 | V147A (GTC→GCC) | yahF | putative NAD(P)‑binding succinyl‑CoA synthase |
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/21); total (17/21) |
GCCACTATTGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGTCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACA > NC_000913/337081‑337351 | gCCACTATTGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGcct < 1:488005/139‑1 (MQ=255) tGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGaa < 2:994395/139‑1 (MQ=255) aGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATg > 2:258501/1‑139 (MQ=255) cgcCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGcct < 2:467406/125‑1 (MQ=255) cgcCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGaa > 2:1096211/1‑139 (MQ=255) atCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGc > 2:327197/1‑139 (MQ=255) cccGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCaa > 2:258206/1‑139 (MQ=255) ggAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTg < 2:1037660/139‑1 (MQ=255) aCCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGCTCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCaa < 2:362112/128‑1 (MQ=255) aCCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCaa > 1:362112/1‑128 (MQ=255) tGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCa < 1:758015/120‑1 (MQ=255) tGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCa > 2:758015/1‑120 (MQ=255) cGGTGATTTCTGTCAACGATCTGTTTGCCGCTCGCGAAGCGCGCCAGGCGCTGCAAAACGATCTCAACGTGATGCTCGTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGGAACTGGCCCACGAAAAAgg > 1:801258/1‑139 (MQ=255) cGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATACTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCtgat > 2:614952/1‑139 (MQ=255) ggTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCtgatg > 1:553886/1‑139 (MQ=255) ggTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCtgatg > 2:769798/1‑139 (MQ=255) aCGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATggg > 1:593580/1‑139 (MQ=255) cGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGgct < 1:478774/114‑1 (MQ=255) cGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGgct > 2:478774/1‑114 (MQ=255) cgcgAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACgg < 2:553886/139‑1 (MQ=255) cgcgAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACgg < 1:801785/139‑1 (MQ=255) gcgAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGc > 1:150221/1‑139 (MQ=255) aGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGAtt < 1:755298/139‑1 (MQ=255) cgTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCa > 2:186790/1‑139 (MQ=255) cAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACggcgcggcgc < 1:796714/139‑1 (MQ=255) cAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATT‑TCAACGGCGCGGCGct < 1:211677/139‑1 (MQ=255) aaaaCGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGct < 2:150221/139‑1 (MQ=255) aaaaCGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGct < 1:1096211/139‑1 (MQ=255) tGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGct > 2:435093/1‑121 (MQ=255) tGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGct < 1:435093/121‑1 (MQ=255) tGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGgc > 1:214763/1‑37 (MQ=255) tGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGgc < 2:214763/37‑1 (MQ=255) cGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAggg > 1:72253/1‑58 (MQ=255) cGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAggg < 2:72253/58‑1 (MQ=255) cGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGc < 1:628561/139‑1 (MQ=255) cGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGc < 2:801258/139‑1 (MQ=255) aTAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCaa < 1:649909/139‑1 (MQ=255) aTAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCaa < 1:327197/139‑1 (MQ=255) aTAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCaa < 1:258501/139‑1 (MQ=255) aaCGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACa < 2:1028825/139‑1 (MQ=255) | GCCACTATTGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGTCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACA > NC_000913/337081‑337351 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |