Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,843,545 C→A E431* (GAG→TAG)  gacS ← sensor protein GacS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,843,5450CA98.6% 236.8 / ‑6.7 70E431* (GAG→TAG) gacSsensor protein GacS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (32/37);  minor base G (0/1);  total (32/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTCGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATT  >  NC_002947/1843401‑1843691
                                                                                                                                                 |                                                                                                                                                   
gCCCCAGACCGGTGCCGCCGGGGTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCtt                                                                                                                                                <  2:591442/150‑1 (MQ=255)
 ccccAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTc                                                                                                                                               <  1:1043203/150‑1 (MQ=255)
  cccAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTcc                                                                                                                                              >  1:1579707/1‑150 (MQ=255)
         cGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATgg                                                                                                                                      >  1:561395/1‑150 (MQ=255)
            tGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGgtgc                                                                                                                                   <  1:1748508/150‑1 (MQ=255)
                         gCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGt                                                                                                                      <  2:1579707/150‑1 (MQ=255)
                           gggCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTCCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGc                                                                                                                    <  1:495992/150‑1 (MQ=255)
                              ccAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCtt                                                                                                                 <  2:1368851/150‑1 (MQ=255)
                                 gTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTcgc                                                                                                              <  2:561395/150‑1 (MQ=255)
                                 gTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTcgc                                                                                                              >  1:1723019/1‑150 (MQ=255)
                                   gAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCgg                                                                                                            <  2:286324/150‑1 (MQ=255)
                                            gCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTGCGCGGGTGAACttc                                                                                                   >  2:839591/1‑149 (MQ=255)
                                              cTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGAt                                                                                                 >  1:1594807/1‑150 (MQ=255)
                                              cTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGAt                                                                                                 >  2:1195993/1‑150 (MQ=255)
                                               tGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATg                                                                                                >  2:1205635/1‑150 (MQ=255)
                                                   tGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGt                                                                                            <  2:1041495/150‑1 (MQ=255)
                                                         ccTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGa                                                                                      <  2:301663/150‑1 (MQ=255)
                                                          cTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGCCGATGGTGCTTTCGCGGGTGAACCTGATGGCGTTGCTGAc                                                                                     <  2:986199/150‑1 (MQ=255)
                                                          cTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGCGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGAc                                                                                     >  2:1466897/1‑150 (MQ=255)
                                                           tGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGAcc                                                                                    >  1:257981/1‑150 (MQ=255)
                                                           tGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGAcc                                                                                    >  2:765413/1‑150 (MQ=255)
                                                              aaCAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAgg                                                                                 >  2:1708771/1‑150 (MQ=255)
                                                                cAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGtt                                                                               >  2:547970/1‑150 (MQ=255)
                                                                cAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGtt                                                                               <  2:1594807/150‑1 (MQ=255)
                                                                   tgtgCGCACATCCTGAGGTGACATGACGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGAAAGACGATGCTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGt                                                                            <  1:748023/150‑1 (MQ=255)
                                                                   tgtgCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGt                                                                            >  1:105087/1‑150 (MQ=255)
                                                                     tgCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGa                                                                          >  1:523476/1‑150 (MQ=255)
                                                                     tgCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGa                                                                          <  1:1127916/150‑1 (MQ=255)
                                                                        gcACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGa                                                                       >  1:1143417/1‑150 (MQ=255)
                                                                         cacaTCCTGAGGTGTCAGGCCGATGCCGGTGTCCTGCACGCTGATGCG‑ATCCGCGAGATCTGGGACATGCAGGAGGGCCTG‑CATGGCCGCCACGGCGATGGTGCCTCCGCGGGCGATTTTGTTGGCGTTGGTGACCAGGTTGGTGAGGAt                                                                      <  1:839591/150‑1 (MQ=255)
                                                                               cTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGc                                                                  >  1:47019/1‑148 (MQ=255)
                                                                               cTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGc                                                                  <  2:47019/148‑1 (MQ=255)
                                                                               cTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCtt                                                                <  1:1708771/150‑1 (MQ=255)
                                                                                tGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGTGTTGCTGACCAGGTTGGTGAGGATCTGCTTg                                                               <  2:1330405/150‑1 (MQ=255)
                                                                                 gAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGa                                                              <  1:341655/150‑1 (MQ=255)
                                                                                  aGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGAGCAGGGTGGTGAGGATCTGCTTGAg                                                             >  2:1636283/1‑150 (MQ=255)
                                                                                        cAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATg                                                                                                >  1:948266/1‑109 (MQ=255)
                                                                                        cAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATg                                                                                                <  2:948266/109‑1 (MQ=255)
                                                                                        cAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCaa                                                       <  2:1380534/150‑1 (MQ=255)
                                                                                         aGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAAc                                                      <  2:523476/150‑1 (MQ=255)
                                                                                         aGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAAc                                                      >  1:862192/1‑150 (MQ=255)
                                                                                          ggCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACg                                                     >  2:347276/1‑150 (MQ=255)
                                                                                           gCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCTTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACgg                                                    >  2:1184948/1‑150 (MQ=255)
                                                                                                tGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGc                                               <  1:1128866/150‑1 (MQ=255)
                                                                                                   cGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTa                                            >  1:805782/1‑150 (MQ=255)
                                                                                                       gtCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAg                                        <  1:1022405/150‑1 (MQ=255)
                                                                                                          cTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAAc                                                                                                      >  2:1134189/1‑85 (MQ=255)
                                                                                                          cTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAAc                                                                                                      <  1:1134189/85‑1 (MQ=255)
                                                                                                          cTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCGGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGa                                     >  2:199669/1‑150 (MQ=255)
                                                                                                              aCGCTGACGCGCAACTTCGCGCAGT‑CTTCGTGCTAGTCTTCCTGTCAAGCAGAAGACGGCATACGTG‑CTGGGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCa                                 <  1:649351/150‑1 (MQ=255)
                                                                                                                 cTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGgt                              <  2:105087/150‑1 (MQ=255)
                                                                                                                 cTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGgt                              <  1:758892/150‑1 (MQ=255)
                                                                                                                 cTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGANCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGgt                              <  1:1156950/150‑1 (MQ=255)
                                                                                                                         cAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGTGGATCTGCTTGAGCCGCAACGGGTCGCCTACCCGCGAAGCAGGTGTGTcgcg                      <  2:257981/150‑1 (MQ=255)
                                                                                                                         cAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTcgcg                      <  1:1195993/150‑1 (MQ=255)
                                                                                                                         cAACTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTcgcg                      <  1:547970/150‑1 (MQ=255)
                                                                                                                          aaCTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCgg                     >  2:1556551/1‑150 (MQ=255)
                                                                                                                          aaCTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCgg                     <  2:598016/150‑1 (MQ=255)
                                                                                                                            cTGCGCGCAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTa                   <  1:199669/150‑1 (MQ=255)
                                                                                                                                gcgcAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATc               >  2:1587772/1‑150 (MQ=255)
                                                                                                                                   cAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCgg                     <  2:259399/141‑1 (MQ=255)
                                                                                                                                   cAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCgg                     >  1:259399/1‑141 (MQ=255)
                                                                                                                                   cAGT‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAgg            <  1:347276/150‑1 (MQ=255)
                                                                                                                                      t‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCa                                         >  1:811129/1‑118 (MQ=255)
                                                                                                                                      t‑CTTCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCa                                         <  2:811129/118‑1 (MQ=255)
                                                                                                                                         ttCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAg       >  1:1356644/1‑150 (MQ=255)
                                                                                                                                         ttCGTGCTAGGCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAg       >  1:1664763/1‑150 (MQ=255)
                                                                                                                                          tCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACgg                                                    >  2:1649162/1‑104 (MQ=255)
                                                                                                                                          tCGTGCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACgg                                                    <  1:1649162/104‑1 (MQ=255)
                                                                                                                                              gCTAGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAAtt  >  1:1701733/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGT‑CTTCGTGCTCGTCTTCCAG‑CATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATT  >  NC_002947/1843401‑1843691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑