Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,852,202	G→T	V416F (GTT→TTT)	relA →	ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,852,202	0	G	T	100.0%	105.6 / NA	36	V416F (GTT→TTT)	relA	ATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/18);  total (18/18)

TACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTG  >  NC_002947/1852058‑1852333                                                                                                                                                 |                                                                                                                                    tACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTCCGAAGAAGAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCAGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTAt                                                                                                                                <  2:82234/150‑1 (MQ=255) tACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTAt                                                                                                                                <  2:735177/150‑1 (MQ=255)      gggCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGtt                                                                                                                           >  2:989551/1‑150 (MQ=255)       ggCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTc                                                                                                                          <  1:166790/150‑1 (MQ=255)                 tCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACg                                                                                                                <  2:844288/150‑1 (MQ=255)                  caggCCCAGCTGCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCTTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACgg                                                                                                               <  2:955557/147‑1 (MQ=255)                       ccAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACg                                                                                                          >  1:436597/1‑150 (MQ=255)                            tCCAACCACTACTAATAAAAGATTGCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAATAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTGTTATGTGTTCACCCCCGACGGCCACGCCATc                                                                                                     <  2:279402/150‑1 (MQ=255)                              cAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGa                                                                                                   >  1:279402/1‑150 (MQ=255)                                  cACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTg                                                                                               >  2:1094455/1‑150 (MQ=255)                                                gATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGcgccac                                                                                 <  1:630874/150‑1 (MQ=255)                                                 aTTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCAcc                                                                                                                       >  1:319588/1‑111 (MQ=255)                                                 aTTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCAcc                                                                                                                       <  2:319588/111‑1 (MQ=255)                                                      caggTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCAtt                                                                           >  1:259475/3‑150 (MQ=255)                                                      cTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGAc                                                                                                  >  2:727654/1‑127 (MQ=255)                                                      cTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGAc                                                                                                  <  1:727654/127‑1 (MQ=255)                                                      cTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCAtt                                                                           <  2:436597/150‑1 (MQ=255)                                                       tGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGGCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTg                                                                          >  1:809075/1‑150 (MQ=255)                                                              gTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCg                                                                   >  1:343252/1‑150 (MQ=255)                                                               tCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGc                                                                  >  1:208724/1‑150 (MQ=255)                                                                    tGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTAcc                                                             <  1:989551/150‑1 (MQ=255)                                                                                aCGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGc                                                                  >  2:578956/1‑133 (MQ=255)                                                                                aCGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCg                                                 >  1:626544/1‑150 (MQ=255)                                                                                aCGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCg                                                 >  1:345790/1‑150 (MQ=255)                                                                                aCGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTCTCCAAAGGCGCCACGCCATTGGACTTCGc                                                                  <  1:578956/133‑1 (MQ=255)                                                                                        cTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGc                                         <  2:259475/150‑1 (MQ=255)                                                                                         tGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGc                                                                                                         >  2:680472/1‑85 (MQ=255)                                                                                         tGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGc                                                                                                         <  1:680472/85‑1 (MQ=255)                                                                                                             gccGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACGTCGCCTACCGCGTGCACACCGAGATCGGCCCCACCTGCCGCGGcgcgcaa                    >  2:297356/1‑147 (MQ=255)                                                                                                                   cAGTGGCGGGGCGGCAGGGAGCGTGACCGGTTTTGTGTGTTCACCCCCGGCGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGTACTTCGCCTACCGCGGGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAAc              <  2:1076712/150‑1 (MQ=255)                                                                                                                     gTTGCGGGTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACgg            >  1:747229/1‑150 (MQ=255)                                                                                                                           ggTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTAt      <  1:1120458/150‑1 (MQ=255)                                                                                                                            gTCGACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATc     >  1:1313665/1‑150 (MQ=255)                                                                                                                               gACATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTg  <  2:636549/150‑1 (MQ=255)                                                                                                                                 cATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCaa               >  2:267015/1‑135 (MQ=255)                                                                                                                                 cATCGAGCCTGACCGGTTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCaa               <  1:267015/135‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                    TACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTG  >  NC_002947/1852058‑1852333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑