Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	473,705	T→C	13.7%	Q428R (CAG→CGG)	dnaG ←	DNA primase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	473,705	0	T	C	13.7%	138.6 / 10.4	57	Q428R (CAG→CGG)	dnaG	DNA primase
Reads supporting (aligned to +/- strand):  ref base T (22/27);  new base C (4/4);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCTGCTCCTGATGAACGGGGGCATAGTCGTGCTGCGGCATGTCGCCGTAGTCGGGGGTGTAGCTGGCCATGGCGTCGTAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATCAACGGTGCGGCCAAGGTCGCCATATGCGCCTTGCCTTCCAGCGAGCGCGGGTCGGC  >  NC_002947/473558‑473841                                                                                                                                                    |                                                                                                                                         gCCTGCTCCTGATGAACGGGGGCATAGTCGTGCTGCGGCATGTCGCCGTAGTCGGGGGTGTAGCTGGCCATGGCGTCGTAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACctgc                                                                                                                                        >  2:383592/1‑150 (MQ=255)      cTCCTGACGAACGGGGGCATAGTCGTGCTGCGGCATGTATCCGTAGTCGGGGGTGTAGCTGGCCATGGCGTCGTAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCgg                                                                                                                                   <  1:727563/150‑1 (MQ=255)       tCCTGATGAACGGGGGCATAGTCGTGCTGCGGCATGTCGCCGTAGTCGGGGGTGTAGCTGGCCATGGCGTCGTAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCggg                                                                                                                                  <  2:1417443/150‑1 (MQ=255)       tCCTGACGAACGGGGGCATAGTCGTGCTGCGGCATGTCTCCGTAGTCGGGGGTCTAGCTGGCCATGGCGTCGTAATCGTAGCCAGGGTCGGAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCggg                                                                                                                                  <  1:492632/150‑1 (MQ=255)        ccTGATGAACGGGGGCATAGTCGTGCTGCGGCATGTCGCCGTAGTCGGGGGTGTAGCTGGCCATGGCGTCGTAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGt                                                                                                                                 <  1:990727/150‑1 (MQ=255)        ccTGATGAACGGGGGCATAGTCGTGCTGCGGCATGTCGCCGTAGTCGGGGGTGTAGCTGGCCATGGCGTCGTAATCGTAGCAAGGGTCGTAGTCCTGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGt                                                                                                                                 <  1:391692/150‑1 (MQ=255)            atgaACGGGGGCATAGTCGTGCTGCGGCATGTCGCCGTAGTCGGGGGTGTAGCTGGCCATGGCGTCGTAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAg                                                                                                                             <  2:258120/150‑1 (MQ=255)                  gggggCATAGTCGTGCTGCGGCATGTCGCCGTAGTCGGGGGTGTAGCTGGCCATGGCGTCGTAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGt                                                                                                                       <  2:23675/150‑1 (MQ=255)                   ggggCATAGTCGTGGTGCGGCATGTCGCCGTAGTCGGGGGTGTAGCTGGCCATGGCGTCGTAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTg                                                                                                                      >  2:1063496/1‑150 (MQ=255)                      gCATAGTCGTGCTGCGGCATGTCGCCGTAGTCGGGGGTGTAGCTGGCCATGGCGTCGTAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGAtt                                                                                                                   >  2:854286/1‑150 (MQ=255)                             cGTGCTGCGGCATGTCGCCGTAGTCGGGGGTGTAGCTGGCCATGGCGTCGTAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCa                                                                                                            <  2:175359/150‑1 (MQ=255)                                      gCATGTCGCCGTAGTCGGGGGTGTAGCTGGCCATGGCGTCGTAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTgc                                                                                                   <  1:383592/150‑1 (MQ=255)                                      gCATGTCGCCGTAGTCGGGGGTGTAGCTGGCCATGGCGTCGTAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTAGTAGGCGGTACTTGTTGGCCCTACCGTCTGACCCGTTAGGTGTTGAGGCCGGTGATTTCCTTCAGGCGGTTgc                                                                                                   <  1:739089/150‑1 (MQ=255)                                        aTGTCGCCGTAGTCGGGGGTGTAGCTGGCCATGGCGTCGTAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCg                                                                                                                         <  2:1276007/126‑1 (MQ=255)                                        aTGTCGCCGTAGTCGGGGGTGTAGCTGGCCATGGCGTCGTAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCg                                                                                                                         >  1:1276007/1‑126 (MQ=255)                                          gTCGCCGTAGTCGGGGGTGTAGCTGGCCATGGCGTCGTAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCAt                                                                                               >  2:587248/1‑150 (MQ=255)                                            cGCCGTAGTCGGGGGTGTAGCTGGCCATGGCGTCGTAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCa                                                                                             <  1:1297794/150‑1 (MQ=255)                                              ccGTAGTCGGGGGTGTAGCTGGCCATGGCGTCGTAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGc                                                                                           >  1:1346821/1‑150 (MQ=255)                                               cGTAGTCGGGGGTGTAGCTGGCCATGGCGTCGTAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCt                                                                                          >  2:386806/1‑150 (MQ=255)                                                            gtAGCTGGCCATGGCGTCGTAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGgc                                                                             <  1:598438/150‑1 (MQ=255)                                                                           gtcgtAATCGTAGCCAGGGTCGTCGTCCGGTCTGTTGCTGGTGGTCTTCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTc                                                              <  2:866936/150‑1 (MQ=255)                                                                           gtcgtAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTc                                                              <  1:854286/150‑1 (MQ=255)                                                                             cgtAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGa                                                            <  1:587248/150‑1 (MQ=255)                                                                              gtAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTTGCGCCGGGGATCTTTTCGAt                                                           >  2:497610/1‑150 (MQ=255)                                                                               tAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCttt                                                                >  2:255596/1‑144 (MQ=255)                                                                               tAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCttt                                                                <  1:255596/144‑1 (MQ=255)                                                                               tAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATc                                                          >  2:288845/1‑150 (MQ=255)                                                                               tAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATc                                                          >  1:206046/1‑150 (MQ=255)                                                                                    gTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAg                                                                                                           >  1:954265/1‑96 (MQ=255)                                                                                    gTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAg                                                                                                           <  2:954265/96‑1 (MQ=255)                                                                                    gTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATCAACgg                                                     >  1:47606/1‑150 (MQ=255)                                                                                          aGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATCAACGGTGCGGc                                               <  1:1063496/150‑1 (MQ=255)                                                                                             gTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATCAACGGTGCGGCCaa                                            <  2:1346821/150‑1 (MQ=255)                                                                                                gTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCCGCAGCAGGTCGAg                                                                                                                             <  1:97747/66‑1 (MQ=38)                                                                                                gTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCCGCAGCAGGTCGAg                                                                                                                             >  2:97747/1‑66 (MQ=38)                                                                                                    tCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATCAACGGTGCGGCCAAGGTCGcc                                     >  2:1043302/1‑150 (MQ=255)                                                                                                      tggCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATCaa                                                        >  2:1382585/2‑129 (MQ=255)                                                                                                      tggCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATCaa                                                        <  1:1382585/128‑1 (MQ=255)                                                                                                              tgctgGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATCAACGGTGCGGCCAAGGTCGCCATATGCgcct                           >  1:974100/1‑150 (MQ=255)                                                                                                                    tCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCCTCTCGATCAACGGTGCGGCCAAGCTCGGCATGTGCgccttgcctt                     <  2:703250/150‑1 (MQ=255)                                                                                                                     cGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATCAACGGTGCGGCCAAGGTCGCCATATGCGCCTTGCCTTc                    <  2:974100/150‑1 (MQ=255)                                                                                                                       ccGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATCAACGGTGCGGCCAAGGTCGCCATATGCGCCTTGCCTTccc                  <  2:206046/150‑2 (MQ=255)                                                                                                                        cGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATCAACGGTGCGGCCAAGGTCGCCATATGCGCCTTGCCTTCCag                 >  2:686548/1‑150 (MQ=255)                                                                                                                          gcgcTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCAt                                                                                               <  2:211542/70‑1 (MQ=255)                                                                                                                          gcgcTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCAt                                                                                               >  1:211542/1‑70 (MQ=255)                                                                                                                            gcTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATCAACGGTGCGGCCAAGGTCGCCATATGCgcc                            <  2:56744/135‑1 (MQ=255)                                                                                                                            gcTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATCAACGGTGCGGCCAAGGTCGCCATATGCgcc                            >  1:56744/1‑135 (MQ=255)                                                                                                                            gcTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGAAGGGCGTCTTTTAGGTAAAGAGTGTGGCCAAGGGCGTCATATGCGCCTTGCGTTCCagcgag             >  2:310928/1‑150 (MQ=255)                                                                                                                                 ttgCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTAGGCACCGGGGACCTGTTCGATCAACGGGGCGGCCAAGGTCGCCATATGCGCCTTGCCTTCCAGCGAGCGCgg        >  1:169033/1‑150 (MQ=255)                                                                                                                                      ccAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATCAACGGTGCGGCCAAGGTCGCCATATGCGCCTTGCCTTCCAGCGAGCGCGGGTCgg   >  1:988685/1‑150 (MQ=255)                                                                                                                                       cAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATCAACGGTGCGGCCAAGGTCGCCATATGCGCCTTGCCTTCCAGCGAGCGCGGGTCggt  <  1:497610/150‑2 (MQ=255)                                                                                                                                         cggcctCGACCCGCAGCAGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATCAACGGTGCGGCCAAGGTCGCCATATgc                               >  2:86557/5‑119 (MQ=255)                                                                                                                                         cggcctCGACCCGCAGCAGGGCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATCAACGGTGCGGCCAAGGTCGCCATATgc                               <  1:86557/115‑1 (MQ=255)                                                                                                                                            cctCGACCCGCAGCAGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATCAACGGTGCGGCCAAGGTCGCCATATGCGCCTTGCCTTCCAGCGAGCGCGGGTCGGc  >  2:625592/2‑145 (MQ=255)                                                                                                                                            cctCGACCCGCAGCAGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATCAACGGTGCGGCCAAGGTCGCCATATGCGCCTTGCCTTCCAGCGAGCGCGGGTCGGc  <  1:625592/144‑1 (MQ=255)                                                                                                                                            ccccGACCCGCAGCAGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATCAACGGTGCGGCCAAGGTCGCCATATGCGCCTTGCCTTCCAGCGAGCGCGGGt      >  1:1134080/4‑141 (MQ=255)                                                                                                                                             ctCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGa                                                            >  2:163047/1‑86 (MQ=255)                                                                                                                                             ctCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGa                                                            <  1:163047/86‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                         GCCTGCTCCTGATGAACGGGGGCATAGTCGTGCTGCGGCATGTCGCCGTAGTCGGGGGTGTAGCTGGCCATGGCGTCGTAATCGTAGCCAGGGTCGTAGTCCGGCATGCTGCTGGTCGCCGGCGCTTGTTGCGCCAGCTGCTCGACCTGCTGCGGGTCGAGGCCGGTGATTTCCTTCAGGCGGTTGCGCATCAGCTGACGCAGGTTGGCGCCGGGGATCTTTTCGATCAACGGTGCGGCCAAGGTCGCCATATGCGCCTTGCCTTCCAGCGAGCGCGGGTCGGC  >  NC_002947/473558‑473841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑