Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_002947	1,843,043	(GAT)2→3	coding (1793/2754 nt)	gacS ←	sensor protein GacS

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 1843036	2 (0.070)	35 (1.150) +TGA	27/266	0.2	93.3%	coding (1800/2754 nt)	gacS	sensor protein GacS
	?	NC_002947	1843037 =	3 (0.100)					coding (1799/2754 nt)	gacS	sensor protein GacS

CCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/1842888‑1843036 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCA  >  NC_002947/1843037‑1843185                                                                                                                                                      |||                                                                                                                                                      CCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCT                                                                                                                                                         >  2:656342/1‑150 CCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCT                                                                                                                                                         >  2:176086/1‑150  CAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTG                                                                                                                                                        >  1:70760/1‑150   AGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGA                                                                                                                                                       <  1:504900/150‑1      TCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGA                                                                                                                                                    <  2:70760/150‑1         AGCAAGAGGCGCCGCAGCTTGCGGGGGGGGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGA                                                                                                                                                 >  1:450745/1‑150                GGCGCCGCAGCTGGGGGGGGGGGGGGGGGGTGGGCGGCAACTGGCCATGTGCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACAGCATTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGC                                                                                                                                          <  2:365267/150‑1                          CTTGCGGGGGCCGGTCGGCTTGGACAGCAATTGGCCATGGGCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTNCTC                                                                                                                                <  2:447519/150‑1                                  TGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACA                                                                                                                        <  1:170020/150‑1                                              TGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGT                                                                                                            <  2:173558/150‑1                                               GGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTT                                                                                                           <  1:667285/150‑1                                                   AGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGT                                                                                                            >  1:142322/1‑145                                                   AGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGT                                                                                                            <  2:142322/145‑1                                                      AACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCG                                                                                                    >  1:134867/1‑150                                                       ACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGA                                                                                                   <  1:239499/150‑1                                                          GGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCA                                                                                                <  2:266721/150‑1                                                           GCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCAC                                                                                               <  1:176086/150‑1                                                                 GCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAA                                                                                         >  2:321986/1‑150                                                                   CGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACT                                                                                       >  1:356827/1‑150                                                                                     GGTACAGCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGGGAAGATTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCT                                                                     <  2:530790/150‑1                                                                                           GCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGG                                                               >  1:639121/1‑150                                                                                             GCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCG                                                             <  2:356827/150‑1                                                                                                   TCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGT                                                                                                            <  2:21004/97‑1                                                                                                   TCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGT                                                                                                            >  1:21004/1‑97                                                                                                     GGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGA                                                                                                                                                    >  1:497473/1‑55                                                                                                     GGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGA                                                                                                                                                    <  2:497473/55‑1                                                                                                          TCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCT                                                >  1:565277/1‑150                                                                                                                          GCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAA                                                                                   >  1:406187/1‑99                                                                                                                          GCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAA                                                                                   <  2:406187/99‑1                                                                                                                          GCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTGGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTGCAGCAACTGGTCGTA                                >  1:164953/1‑150                                                                                                                            ATTGGCAATGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCAGCACCCCCTGCAGCAACTGGTCGTAGG                              >  2:378691/1‑150                                                                                                                             TTGGCAGTGGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTGCAGCAACTGGTCGTAGGA                             >  2:469437/1‑150                                                                                                                                     GGTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTGCAGCAACTGGTCGTAGGAGGCGAACA                     >  1:413202/1‑150                                                                                                                                      GTAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAG                    <  2:134867/150‑1                                                                                                                                       TAACGCTCGAGCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAGG                   <  2:565277/150‑1                                                                                                                                                 GCTGCTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGA         <  2:639121/150‑1                                                                                                                                                     CTGATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAAGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCC     >  1:407678/1‑150                                                                                                                                                        ATGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCA  <  1:321986/150‑1                                                                                                                                                      |||                                                                                                                                                      CCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/1842888‑1843036 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCA  >  NC_002947/1843037‑1843185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.