| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 195,634 | 0 | C | T | 43.9% | 7.1 / 41.1 | 41 | T380T (ACC→ACT) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base C (12/11); new base T (12/6); total (24/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGG > NC_002947/195489‑195779 | aTTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACcg > 2:569063/1‑150 (MQ=255) cccGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATAAACGACTCGGTCGACACCACCACCGCGACCCTGAc > 1:610575/1‑150 (MQ=255) cGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCCCAGACCACCATCAACCACTCGGTCGACACCACCACCGCGCCCCTGACGGCGAGCCCCTc > 2:224717/1‑150 (MQ=11) caacaaCGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGTCACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCAc > 2:413225/1‑150 (MQ=12) gTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcg > 1:433498/1‑150 (MQ=38) ccGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCTGTCGACACCACCACCGCGACCCTGTGGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGa < 2:156938/150‑1 (MQ=255) cAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGGCTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAc < 2:413781/150‑1 (MQ=255) cGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑a < 2:640255/150‑1 (MQ=40) aCCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACAcc < 1:459554/150‑1 (MQ=255) cacaggcggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAg < 1:609560/112‑1 (MQ=255) cacaGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAg > 2:609560/1‑116 (MQ=16) ggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGcc < 1:120571/150‑1 (MQ=12) gAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGCTCAACGACTCGGTCGACACCACCACCGCGACCCGGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCg < 1:226295/150‑1 (MQ=255) cgtaCCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCTCCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGAcc < 1:423257/147‑1 (MQ=11) cTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAAcc > 2:2205/1‑150 (MQ=40) ccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGt < 1:405214/80‑1 (MQ=255) ccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGt > 2:405214/1‑80 (MQ=255) ccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAAc > 1:319305/1‑150 (MQ=255) ccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAAc > 2:166914/1‑150 (MQ=255) ccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCT‑ACACCGTGACCCTGAg < 2:76663/119‑1 (MQ=14) ccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCT‑ACACCGTGACCCTGAg > 1:76663/1‑119 (MQ=17) gACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTc > 1:668743/1‑147 (MQ=255) gACCCCGGCTCAGACCACGATCAACGACTCGGACGACCCCACTGCCGCGACCCTGACGGCCAGCCCGTGGGTCACCGAAGGCGGCGTGATCACTT‑ACAACGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCaa > 2:698729/1‑150 (MQ=14) gACCCCGGCTCAGACCACGATCAACGACTCCGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGATCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCGGTCCaa > 2:698776/1‑150 (MQ=255) ccGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGc > 1:114213/1‑150 (MQ=255) aCGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGc > 2:447426/1‑138 (MQ=255) aCGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGc < 1:447426/138‑1 (MQ=255) gATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCGCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGt > 1:521142/1‑150 (MQ=255) gATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTa > 1:377123/1‑146 (MQ=255) gATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTa < 2:377123/146‑1 (MQ=255) gATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGG‑GAATTAACTTCTTACCAGAACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGt > 2:203592/1‑150 (MQ=14) aCGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAg > 1:162944/1‑150 (MQ=255) cGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGc < 2:319305/150‑1 (MQ=255) gACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCg < 1:166914/150‑1 (MQ=255) aCTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg < 1:265014/150‑1 (MQ=255) tCGGTCGACACCACTACCGCGACCCTGACGGCGAACCCGTCGGTCACCGAAGGCGGCCTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCgggg > 1:57031/1‑149 (MQ=255) tCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGt > 2:259977/1‑150 (MQ=255) tCGTCACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTaaaa < 1:413225/150‑1 (MQ=255) cGACACCACCACCGCGACCCTGACGGCGAGCCCGCCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAAc < 1:224717/150‑1 (MQ=255) gatgccaccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGCGACCCTGAGCAAc > 2:157/5‑81 (MQ=0) gatgccaccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGCGACCCTGAGCAAc < 1:157/77‑1 (MQ=0) acacCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACAATTACCGTTGAAGCGGGTAAAAccc > 1:250297/1‑150 (MQ=255) acacCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAAccc > 2:528174/1‑150 (MQ=255) accaccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAg < 1:569063/150‑1 (MQ=255) ccactaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAgg < 1:692072/150‑1 (MQ=255) cactaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAg > 1:229704/1‑148 (MQ=255) caccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg > 1:481413/1‑137 (MQ=255) caccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg < 2:481413/137‑1 (MQ=255) accaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTg < 1:422740/79‑1 (MQ=40) | ATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTT‑ACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGG > NC_002947/195489‑195779 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |