| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,397,705 | A→T | 24.1% | L223F (TTA→TTT) | hsdS → | type I restriction modification system specificity protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,397,705 | 0 | A | T | 24.1% | 50.4 / 16.3 | 29 | L223F (TTA→TTT) | hsdS | type I restriction modification system specificity protein |
| Reads supporting (aligned to +/- strand): ref base A (13/9); new base T (4/3); total (17/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.54e-01 | |||||||||||
CCGGCGCCAACATTGCCAGAGCTATTTGCCGTGTAGTCCCCAGTGTGCATGTCTCGAAGGACTACATAATTTGGTTGCTACAAAGTGACCTTATGCGAAAGCAGTTCTTGGGAGATACTCGAACCCTCGCCCAGCCAACATTAAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGAACAACACCGCATCGTTGCCAAAGTCGAAGAACTAATGGCCCTATGCGACCGCTTGGAAGCCCAGCAGGCCGACGCCGAGTCCGCCCACGTC > NC_002947/5397563‑5397846 | ccGGCGCCAACATTGCCAGAGCTATTTGCCGTGTAGTCCCCAGTGTGCATGTCTCGAAGGACTACATAATTTGGTTGCTACAAAGTGACCTTATGCGAAAGCAGTTCTTGGGAGATACTCGAACCCTCGCCCAGCCAACATTAAATGTTg < 1:341531/150‑1 (MQ=255) acgcCAACATTGCCAGAGCTATTTGCCGTGTAGTCCCCAGTGTGCATGTCTCGAAGGACTACATAATTTGGTTGCTACAAAGTGACCTTATGCGAAAGCAGTTCTTGGGAGATACTCGAACCCTCGCCCAGCCAACATTAAATGTTGGcc > 2:33885/2‑150 (MQ=255) agagCTATGTGCCGTGTAGTCCCCAGTGTGCATGTCTCGAAGGACTACATAATTTGGTTGCTACAAAGTGACCTTATGCGAAAGCAGTTCTTGGGAGATACTCGAACCCTCGCCCAGCCAACATTAAATGTTGGCCTGATGCGTAGTGcc > 2:816609/1‑150 (MQ=255) cccAGTGTGCATGTCTCGAAGGACTACATAATTTGGTTGCTACAAAGTGACCTTATGCGAAAGCAGTTCTTGGGAGATACTCGAACCCTCGCCCAGCCAACATTTAATGTTGGct > 1:164389/1‑114 (MQ=255) cccAGTGTGCATGTCTCGAAGGACTACATAATTTGGTTGCTACAAAGTGACCTTATGCGAAAGCAGTTCTTGGGAGATACTCGAACCCTCGCCCAGCCAACATTTAATGTTGGct < 2:164389/115‑2 (MQ=255) gAAGGACTACATAATTTGGTTGCTACAAAGTGACCTTATGCGAAAGCAGTTCTTGGGAGATACTCGAACCCTCGCCCAGCCAACATTAAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGAACAACACCGCAt > 1:716233/1‑150 (MQ=255) tACAAAGTGACCTTATGCGAAAGCAGTTCTTGGGAGATACTCGAACCCTCGCCCAGCCAACATTAAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTc < 2:324899/105‑1 (MQ=255) tACAAAGTGACCTTATGCGAAAGCAGTTCTTGGGAGATACTCGAACCCTCGCCCAGCCAACATTAAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTc > 1:324899/1‑105 (MQ=255) tACAAAGTGACCTTATGCGAAAGCAGTTCTTGGGAGATACTCGAACCCTCGCCCAGCCAACATTAAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGAACGACACCGCATCGTTGCCAAAGTCGAAGAACTaa > 2:726102/1‑150 (MQ=255) tACAAAGTGACCTTATGCGAAAGCAGTTCTTGGGAGATACTCGAACCCTCGCCCAGCCAACATTAAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGAACAACACCGCATCGTTGCCAAAGTCGAAGAACTaa > 1:350542/1‑150 (MQ=255) aaaGTGACCTTATGCGAAAGCAGTTCTTGGGAGATACTCGAACCCTCGCCCAGCCAACATTAAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGAACAACACCGCATCGTTGCCAAAGTCGAAGAACTAATgg > 2:576451/1‑150 (MQ=255) cTTATGCGAAAGCAGTTCTTGGGAGATACTCGAACCCTCGCCCAGCCAACATTAAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGAACAACACCGCATCGTTGCCAAAGTCGAAGAACTAATGGCCCTATGc < 1:576451/150‑1 (MQ=255) tATGCGAAAGCAGTTCTTGGGAGATACTCGAACCCTCGCCCAGCCAACATTAAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGAACAACACCGCATCGTTGCCAAAGTCGAAGAACTAATGGCCCTATGCGa < 1:33885/150‑1 (MQ=255) gCGAAAGCAGTTCTTGGGAGATACTCGAACCCTCGCCCACCCAACATTAAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGAACAACACCGCATCGTTGTCAAAGTCGAAGAACTAATGGCCCTATGCGACCg < 1:416075/150‑1 (MQ=255) gAAAGCCGTTCTTGGGAGATACTCGAACCCTCGCCCAGCCAACATTAAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGAACAACACCGCATCGTTGCCAAAGTCGAAGAACTAATGGCCCTATGCGACCGCt > 2:444775/1‑150 (MQ=255) aaGCAGTTCTTGGGAGATACTCGAACCCTCGCCCAGCCAACAGTAAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGAACAACACCGCATCGTTGCCAAAGTCGAAGAAATAATGGCCCTATGCGACCGCTTg > 1:272568/1‑150 (MQ=255) aGCAGTTCTTGGGAGATACTCGAACCCTCGCCCAGCCAACATTAAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGaacaa < 1:326962/98‑1 (MQ=255) aGCAGTTCTTGGGAGATACTCGAACCCTCGCCCAGCCAACATTAAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGaacaa > 2:326962/1‑98 (MQ=255) ttGGGAGATACTCGAACCCTCGCCCAGCCAACATTAAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGAACAACACCGCATCGTTGCCAAAGTCGAAGAACTAATGGCCCTATGCGACCGCTTGGAAGCCcag < 2:350542/150‑1 (MQ=255) tCGAACCCTCGCCCAGCCAACATTAAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGAACAACACCGCATCGTTGCCAAAGTCGAAGAACTAATGGCCCTATGCGACCGCTTGGAAGCCCAGCAGGCCGACGc > 2:845951/1‑150 (MQ=255) ccTCGCCCAGCCAACATTAAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGATCAACACCGCATCGTTGCCAAAGTCGAAGAACTAATGGCCCTATGCGACCGCTTGGAAGCCcagca < 2:241788/135‑1 (MQ=255) ccTCGCCCAGCCAACATTAAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGATCAACACCGCATCGTTGCCAAAGTCGAAGAACTAATGGCCCTATGCGACCGCTTGGAAGCCcagca > 1:241788/1‑135 (MQ=255) gCCCAGCCAACATTAAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGAACAACACCGCATCGTTGCCAAAGTCGAAGAACTAATGGCCCTATGCGACCGCTTGGAAGCCCAGCAGGCCGACGCCGAGTCCGcc < 1:845951/150‑1 (MQ=255) gCCAACATTTAATGTTGGCTTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGAACAACACCGCATCGTTGCCAAAGTCGAAGAACTAATGGCCCTATGCGACCGCTTGGAAGCCCAGCAGGCCGACGCCGAGTCCGc < 2:86563/144‑1 (MQ=255) gCCAACATTTAATGTTGGCTTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGAACAACACCGCATCGTTGCCAAAGTCGAAGAACTAATGGCCCTATGCGACCGCTTGGAAGCCCAGCAGGCCGACGCCGAGTCCGc > 1:86563/1‑144 (MQ=255) gCCAACATTTAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGAACAACACCGCATCGTTGCCAAAGTCGAAGAACTAATGGCCCTATGCGAc < 2:278078/109‑1 (MQ=255) gCCAACATTTAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGAACAACACCGCATCGTTGCCAAAGTCGAAGAACTAATGGCCCTATGCGAc > 1:278078/1‑109 (MQ=255) gCCAACATTTAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGAACAACACCGCATCGTTGCCAAAGTCGAAGAACTAATGGCCCTATGCGACCGCTTGGAAGCCCAGCAGGCCGACGCCGAGTCCGCCCACGt > 1:380846/1‑150 (MQ=255) ccAACATTAAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGAACAACACCGCATCGTTGCCAAAGTCGAAGAACTAATGGCCCTATGCGACCGCTTGGAAGCCCAGCAGGCCGACGCCGAGTCCGCCCACGTc > 2:840745/1‑150 (MQ=255) | CCGGCGCCAACATTGCCAGAGCTATTTGCCGTGTAGTCCCCAGTGTGCATGTCTCGAAGGACTACATAATTTGGTTGCTACAAAGTGACCTTATGCGAAAGCAGTTCTTGGGAGATACTCGAACCCTCGCCCAGCCAACATTAAATGTTGGCCTGATTCGTAGTGCCGCGGCACCTCTGCCTCCCCTCGCCGAACAACACCGCATCGTTGCCAAAGTCGAAGAACTAATGGCCCTATGCGACCGCTTGGAAGCCCAGCAGGCCGACGCCGAGTCCGCCCACGTC > NC_002947/5397563‑5397846 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |