Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	2,403,077	G→A	Q10* (CAA→TAA)	PP_2105 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,403,077	0	G	A	100.0%	144.3 / NA	44	Q10* (CAA→TAA)	PP_2105	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/23);  total (21/23)

GTTTCGCGGGCCTGGGCCAGGCGCTCGTGAATCTGCTCGCGCAATTCGTCGGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTGTGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGATGAGTGTTGGTACTTCGAGTGACAGCACGCATGGAAGGTTCAAGTGCGGCTGCTGGTATGGGCCTTGCTATGTTTTTGCCACGTTGCGGAGCGCCTGTGAC  >  NC_002947/2402938‑2403222                                                                                                                                            |                                                                                                                                                  gTTTCGCGGGCCTGGGCCAGGCGCTCGTGAATCTGCTCGCGCAATTCGTCGGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCttt                                                                                                                                         <  2:1326616/150‑1 (MQ=255)  tttCGCGGGCCTGGGCCAGGCGCTCGTGAATCTGCTCGCGCAATTCGTCGGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTg                                                                                                                                        <  1:188939/150‑1 (MQ=255)   ttCGCGGGCCTGGGCCAGGCGCTCGTGAATCTGCTCGCGCAATTCGTCGGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGc                                                                                                                                       <  2:240629/150‑1 (MQ=255)           ccTGGGCCAGGCGCTCGTGAATCTGCTCGCGCAATTCGTCGGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGc                                                                                                                               >  1:1343671/1‑150 (MQ=255)             tGGGCCAGGCGCTCGTGAATCTGCTCGCGCAATTCGTCGGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTg                                                                                                                                        >  1:1097227/1‑139 (MQ=255)             tGGGCCAGGCGCTCGTGAATCTGCTCGCGCAATTCGTCGGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTg                                                                                                                                        <  2:1097227/139‑1 (MQ=255)               ggCCAGGCGCTCGTGAATCTGCTCGCGCAATTCGTCGGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGc                                                                                                                           >  2:1324421/1‑150 (MQ=255)                       gcTCGTGAATCTGCTCGCGCAATTCGTCGGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGtc                                                                                                                   <  1:454484/150‑1 (MQ=255)                             gAATCTGCTCGCGCAATTCGTCGGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGGCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTccc                                                                                                             >  2:1073740/1‑150 (MQ=255)                              aaTCTGCTCGCGCAATTCGTCGGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCt                                                                                                            <  1:776445/150‑1 (MQ=255)                                tCTGCTCGCGCAATTCGTCGGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGTCGATTTGCTGGCCATgg                                                                                                                      <  1:1051761/138‑1 (MQ=255)                                tCTGCTCGCGCAATTCGTCGGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATgg                                                                                                                      >  2:1051761/1‑138 (MQ=255)                                    cTCGCGCAATTCGTCGGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGAt                                                                                                      <  1:37506/150‑1 (MQ=255)                                    cTCGCGCAATTCGTCGGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGAt                                                                                                      >  2:559032/1‑150 (MQ=255)                                         gcAATTCGTCGGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATgg                                                                                                                      <  1:505132/129‑1 (MQ=255)                                         gcAATTCGTCGGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGACAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATgg                                                                                                                      >  2:505132/1‑129 (MQ=255)                                             ttCGTCGGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGATGAGTGTTgg                                                                                             <  2:207500/150‑1 (MQ=255)                                                gtcgGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAATGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCAt                                                                                                                        <  2:1116202/120‑1 (MQ=255)                                                gtcgGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCAt                                                                                                                        >  1:1116202/1‑120 (MQ=255)                                                 tcgGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGATGAGTGTTGGTACt                                                                                         >  2:1455712/1‑150 (MQ=255)                                                    gCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGATGAGTGTTGGTACTTCg                                                                                      >  2:701213/1‑150 (MQ=255)                                                    gCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGGCGCCCACAAAGGTATGGAAATAAGCCATCAATATCTCATATGCATTCTTTGCCCACTTACTGGCCAGGTGTCTCTCCCTGTCCATGAGGGTTGGTACTTCg                                                                                      >  2:575426/1‑150 (MQ=255)                                                          tCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGATGAGGGTTGGTACTTCGAGTGAc                                                                                <  2:1353015/150‑1 (MQ=255)                                                               ggCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGATGAGTGTTGGTACTTCGAGTGACAGCAc                                                                           >  2:717661/1‑150 (MQ=255)                                                                 ccAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGATGAGTGTTGGTACTTCGAGTGACAGCACGc                                                                         >  2:860125/1‑150 (MQ=255)                                                                                         gcTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGATGAGTGTTGGTACTTCGAGTGACAGCACGCATGGAAGGTTCAAGTGCGgctgct                                                 <  1:701213/150‑1 (MQ=255)                                                                                         gcTTCTCGGTGTCCCGCACCAAGGCCTGGAGGTCCGCCATCAATATCTCTTAGGCATGCTTTGCCGATTTGCTGGCCATGGGCCTCTCCCTGTCGATGCGTGTTGGTACTTCGAGTGACAGCTCGCATGGAAGGTTCAAGTGCGgctgct                                                 <  1:575426/150‑1 (MQ=255)                                                                                             ctcGGTGGCGCGCACAAAGGCATGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGATGAGTGTTGGTACTTCGAGTGACAGCACGCATGGAAGGTTAAAGTGCGGCTGCTGGTa                                             >  2:321922/1‑150 (MQ=255)                                                                                              tcGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGATGAGTGTTGGTACTTCGAGTGACAGCACGCATGGAAGGTTCAAGTGCGGCTGCTGGTAt                                            >  1:1109795/1‑150 (MQ=255)                                                                                              tcGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGATGAGTGTTGGTACTTCGAGTGACAGCACGCATGGAAGGTTCAAGTGCGGCTGCTGGTAt                                            >  2:426466/1‑150 (MQ=255)                                                                                                 gtgtCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGATGAGTGTTGGTACTTCGAGTGACAGCACGCATGGAAGGTTCAAGTGCGGCTGCTGGTATggg                                         <  1:426466/150‑1 (MQ=255)                                                                                                    tCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGATGAGTGTTGGTACTTCGAGTGACAGCACGCATGGAAGGTTCAAGTGCGGCTGCTGGTATGGGCCt                                      <  2:1343671/150‑1 (MQ=255)                                                                                                            cAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATTGGTCTCTCCCTGTCGATGAGTGTTGGTACTTCGAGTGACAGCACGCATGGAAGGTTCAAGTGCGGCTGCTGGTATGGGCCTTGCTATGt                              <  2:1477241/150‑1 (MQ=255)                                                                                                            cAAGGCCTGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCTATGAGTGTTGGTACTTCGAGTGACAGCACGCATGGAAGGTTCAAGTGCGGCTGATGCTCTGGGCCTTACtctat                              >  2:322847/1‑146 (MQ=255)                                                                                                                   tGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGATGAGTGTTGGTACTTCGAGTGACAGCACGCATGGAAGGTTCAAGTGCgg                                                      >  2:113118/1‑119 (MQ=255)                                                                                                                   tGGAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGATGAGTGTTGGTACTTCGAGTGACAGCACGCATGGAAGGTTCAAGTGCgg                                                      <  1:113118/119‑1 (MQ=255)                                                                                                                    ggAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCCGTCGATGAGTGTTGGTACTTCGAGTGACAGCACGCATGGAAGGTTCAAGTGCGGCTGCTGGTAt                                            >  2:1263761/1‑128 (MQ=255)                                                                                                                    ggAAGTCAGCCATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCCGTCGATGAGTGTTGGTACTTCGAGTGACAGCACGCATGGAAGGTTCAAGTGCGGCTGCTGGTAt                                            <  1:1263761/128‑1 (MQ=255)                                                                                                                     gAAGTCAGCCATCAATATCTCTTATGCATTATTTGTCGGTTTTCTGGCCATGGGTCTCTCCCTGTCGATGAGTGTTGGTACTTCGAGTGACAGCACGCATGGAAGGTTCAAGTGCGGCTGCTGGTATGGGCCTTGCTATGTTTTTGCCAc                     <  1:1455712/150‑1 (MQ=255)                                                                                                                             ccATCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGATGAGTGTTGGTACTTCGAGTGACAGCACGCATGGAAGGTTCAAGTGCGGCTGCTGGTATGGGCCTTGCTATGTTTTTGCCACGTTGCGGa             >  2:823582/1‑150 (MQ=255)                                                                                                                               aTCAATATCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGATGAGTGTTGGTACTTCGAGTGACAGCACGCATGGAAGGTTCAAGTGCGGCTGCTGGTATGTGCCTTGCTATGTTTTTGCCACGTTGCGGAgc           >  1:670934/1‑150 (MQ=255)                                                                                                                                     atCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGATGAGTGTTGGTACTTCGAGTGACAGCACGCATGGAAGGTTCAAGTGCGGCTGCTGGTATGGGCCTTGCTATGTTTTTGCCACGTTGCGGAGCGCCtgt     <  1:1324421/150‑1 (MQ=255)                                                                                                                                     atCTCTTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGATGAGTGTTGGTACTTCGAGTGACAGCACGCATGGAAGGTTCAAGTGCGGCTGCTGGTATGGGCCTTGCTATGTTTTTGCCACGTTGCGGAGCGCCtgt     <  1:860125/150‑1 (MQ=255)                                                                                                                                        tctTATGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGATGAGTGTTGGTACTTCGAGTGACAGCACGCATGGAAGGTTCAAGTGCGGCTGCTGGTATGGGCCTTGCTATGTTTTTGCCACGTTGCGGAGCGCCTGTGAc  <  1:559032/150‑1 (MQ=255)                                                                                                                                            |                                                                                                                                                  GTTTCGCGGGCCTGGGCCAGGCGCTCGTGAATCTGCTCGCGCAATTCGTCGGCCTGGTCGCCGGCCAGGTTGGCGGTGTCGGCCAGCAGCTTCTCGGTGTCGCGCACCAAGGCCTGGAAGTCAGCCATCAATATCTCTTGTGCATTCTTTGCCGATTTGCTGGCCATGGGTCTCTCCCTGTCGATGAGTGTTGGTACTTCGAGTGACAGCACGCATGGAAGGTTCAAGTGCGGCTGCTGGTATGGGCCTTGCTATGTTTTTGCCACGTTGCGGAGCGCCTGTGAC  >  NC_002947/2402938‑2403222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑