breseq version 0.33.2 revision caddc01d3be9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
read file | reads | bases | passed filters | average | longest | mapped | |
---|---|---|---|---|---|---|---|
errors | Pputida_HGL_58_S263_L002_R1_001.good.fq | 1,919,543 | 257,217,799 | 100.0% | 134.0 bases | 150 bases | 94.0% |
errors | Pputida_HGL_58_S263_L002_R2_001.good.fq | 1,919,543 | 257,217,799 | 100.0% | 134.0 bases | 150 bases | 92.3% |
total | 3,839,086 | 514,435,598 | 100.0% | 134.0 bases | 150 bases | 93.1% |
seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
---|---|---|---|---|---|---|---|
coverage | distribution | NC_002947 | 6,181,929 | 77.4 | 3.1 | 100.0% | Pseudomonas putida KT2440 chromosome, complete genome. |
total | 6,181,929 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
option | limit | actual |
---|---|---|
Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 76261 |
Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 1747 |
Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.087 |
reference sequence | pr(no read start) |
---|---|
NC_002947 | 0.77286 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
option | value |
---|---|
Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
option | value |
---|---|
Mode | Consensus/Mixed Base |
Ploidy | 1 (haploid) |
Consensus mutation E-value cutoff | 10 |
Consensus frequency cutoff | 0.75 |
Consensus minimum variant coverage each strand | OFF |
Consensus minimum total coverage each strand | OFF |
Consensus minimum variant coverage | OFF |
Consensus minimum total coverage | OFF |
Polymorphism E-value cutoff | 10 |
Polymorphism frequency cutoff | 0.2 |
Polymorphism minimum variant coverage each strand | OFF |
Polymorphism minimum total coverage each strand | OFF |
Polymorphism minimum variant coverage | OFF |
Polymorphism minimum total coverage | OFF |
Polymorphism bias cutoff | OFF |
Predict indel polymorphisms | YES |
Skip indel polymorphisms in homopolymers runs of | OFF |
Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
program | version |
---|---|
bowtie2 | 2.3.4.1 |
R | 3.4.4 |
step | start | end | elapsed |
---|---|---|---|
Read and reference sequence file input | 01:47:16 21 Aug 2019 | 01:48:50 21 Aug 2019 | 1 minute 34 seconds |
Read alignment to reference genome | 01:48:50 21 Aug 2019 | 02:02:51 21 Aug 2019 | 14 minutes 1 second |
Preprocessing alignments for candidate junction identification | 02:02:51 21 Aug 2019 | 02:04:14 21 Aug 2019 | 1 minute 23 seconds |
Preliminary analysis of coverage distribution | 02:04:14 21 Aug 2019 | 02:06:54 21 Aug 2019 | 2 minutes 40 seconds |
Identifying junction candidates | 02:06:54 21 Aug 2019 | 03:37:16 21 Aug 2019 | 1 hour 30 minutes 22 seconds |
Re-alignment to junction candidates | 03:37:16 21 Aug 2019 | 03:42:08 21 Aug 2019 | 4 minutes 52 seconds |
Resolving best read alignments | 03:42:08 21 Aug 2019 | 03:44:15 21 Aug 2019 | 2 minutes 7 seconds |
Creating BAM files | 03:44:15 21 Aug 2019 | 03:46:40 21 Aug 2019 | 2 minutes 25 seconds |
Tabulating error counts | 03:46:40 21 Aug 2019 | 03:47:53 21 Aug 2019 | 1 minute 13 seconds |
Re-calibrating base error rates | 03:47:53 21 Aug 2019 | 03:47:55 21 Aug 2019 | 2 seconds |
Examining read alignment evidence | 03:47:55 21 Aug 2019 | 04:03:04 21 Aug 2019 | 15 minutes 9 seconds |
Polymorphism statistics | 04:03:04 21 Aug 2019 | 04:03:06 21 Aug 2019 | 2 seconds |
Output | 04:03:06 21 Aug 2019 | 04:04:51 21 Aug 2019 | 1 minute 45 seconds |
Total | 2 hours 17 minutes 35 seconds |