Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	6,070,587	A→G	G38G (GGT→GGC)	PP_5324 ←	two‑component system response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,070,587	0	A	G	100.0%	153.6 / NA	47	G38G (GGT→GGC)	PP_5324	two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/22);  total (25/22)

CTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATCCCGACTAGC  >  NC_002947/6070441‑6070727                                                                                                                                                   |                                                                                                                                             cTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCg                                                                                                                                           <  1:398040/150‑1 (MQ=255)     tCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGttaa                                                                                                                                       <  1:562581/150‑3 (MQ=255)       aCCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACCGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATa                                                                                                                                     >  2:694004/1‑150 (MQ=255)            gatgaACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGcgtc                                                                                                                                >  1:1200085/1‑150 (MQ=255)            gatgaACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCACACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGcgtc                                                                                                                                >  1:457090/1‑150 (MQ=255)              tgaACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGcgtcgt                                                                                                                              >  1:958316/1‑150 (MQ=255)               gaACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCgtcgtc                                                                                                                             >  2:1194106/1‑150 (MQ=255)                  cTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGAt                                                                                                                          <  1:251889/150‑1 (MQ=255)                            ttcAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATAccc                                                                                                                >  1:552618/1‑150 (MQ=255)                             tcAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCg                                                                                                               <  2:1200085/150‑1 (MQ=255)                             tcAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCg                                                                                                               <  1:454860/150‑1 (MQ=255)                                gTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAGGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGa                                                                                                            >  2:1132231/1‑150 (MQ=255)                                        tgctgACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTa                                                                                                    >  1:567480/1‑150 (MQ=255)                                           tgACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGc                                                                                                 >  2:269792/1‑150 (MQ=255)                                            gACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCa                                                                                                <  1:481938/150‑1 (MQ=255)                                                 cACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCa                                                                                                       <  1:1013750/138‑1 (MQ=255)                                                 cACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCa                                                                                                       >  2:1013750/1‑138 (MQ=255)                                                       gTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCgg                                                                                     <  1:868290/150‑1 (MQ=255)                                                        tCAGCAGGTGCAAGCTAGACGGTTCCGGGTTGTCGCAGTCGCACGGGACCAGGTCGAACGCTTCCTTGCCGAGCATGTCCATGGCCTTGCGGCCGTTGATAACGTCGTTGATGGCCATTCCCGGGAAGGCACTACGCAGACACTTGCggg                                                                                    >  2:338849/1‑149 (MQ=255)                                                           gCAATTCCAATCCAGACCTTTACACCATTTTTCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATTGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCa                                                                                 <  2:611811/150‑1 (MQ=255)                                                                  cAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGAt                                                                                                                          >  2:1049315/1‑102 (MQ=255)                                                                  cAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGAt                                                                                                                          <  1:1049315/102‑1 (MQ=255)                                                                  cAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGACCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACg                                                                          >  2:1151136/1‑150 (MQ=255)                                                                     gCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGa                                                                                                                           >  2:237426/1‑98 (MQ=255)                                                                     gCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGa                                                                                                                           <  1:237426/98‑1 (MQ=255)                                                                      ccAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATa                                                                      >  2:662335/1‑150 (MQ=255)                                                                          aCATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACg                                                                  <  1:1194106/150‑1 (MQ=255)                                                                            aTTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACggg                                                                <  2:958316/150‑1 (MQ=255)                                                                                  ggCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCa                                                          <  2:929576/150‑1 (MQ=255)                                                                                     aTTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATcc                                                       >  2:373536/1‑150 (MQ=255)                                                                                     aTTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATcc                                                       >  2:387042/1‑150 (MQ=255)                                                                                       ttCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCAc                                                     >  2:720513/1‑150 (MQ=255)                                                                                          ccAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGAc                                                  <  1:720513/150‑1 (MQ=255)                                                                                            aGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCa                                                <  1:387042/150‑1 (MQ=255)                                                                                                  acaGGACCAGGTCGAACGCTTCCTTGCNGAGCATGGCCATNGCCTTGNGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACAt                                          >  1:1050788/1‑150 (MQ=255)                                                                                                  acaGGACCAGGTCGAACGCTTCATTGCCGAGCAGGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACCCAGACACTCCCGGACCACATCACGGATAAACGGGACGGCATCCACCACCAGCACAt                                          >  2:265451/1‑150 (MQ=255)                                                                                                         cAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACttt                                   >  2:380357/1‑150 (MQ=255)                                                                                                           ggTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTAc                                 >  2:306590/1‑150 (MQ=255)                                                                                                              cGAACGCTTCCTTGCCGAGCATGGCCATGGCCATGCGGCAGTAGAACACATCGGCGATGTCCATACCCGGGACGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCa                              <  1:285545/150‑1 (MQ=255)                                                                                                                aaCGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATc                            >  1:681961/1‑150 (MQ=255)                                                                                                                            ccGAGCATGGCCATTGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCtttt                <  1:890510/150‑1 (MQ=255)                                                                                                                               aGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGaa             <  2:457090/150‑1 (MQ=255)                                                                                                                               aGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGaa             <  2:567480/150‑1 (MQ=255)                                                                                                                                 catggccatggcCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATc           <  1:306590/150‑1 (MQ=255)                                                                                                                                     gccatggccTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATCCCGa       >  1:146339/1‑150 (MQ=255)                                                                                                                                      ccatggccTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATCCCGAc      >  2:914671/1‑150 (MQ=255)                                                                                                                                          ggccTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATCCCGACTAGc  <  2:681961/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                             CTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATCCCGACTAGC  >  NC_002947/6070441‑6070727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑