breseq  version 0.33.2  revision caddc01d3be9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsPputida_HGL_59_S323_L002_R1_001.good.fq1,270,204170,651,760100.0%134.3 bases150 bases93.8%
errorsPputida_HGL_59_S323_L002_R2_001.good.fq1,270,204170,651,760100.0%134.3 bases150 bases92.0%
total2,540,408341,303,520100.0%134.3 bases150 bases92.9%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0029476,181,92951.22.6100.0%Pseudomonas putida KT2440 chromosome, complete genome.
total6,181,929100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 10000049528
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 3
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000762
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.038

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0029470.83942

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input14:42:44 21 Aug 201914:43:47 21 Aug 20191 minute 3 seconds
Read alignment to reference genome14:43:48 21 Aug 201914:53:12 21 Aug 20199 minutes 24 seconds
Preprocessing alignments for candidate junction identification14:53:12 21 Aug 201914:54:07 21 Aug 201955 seconds
Preliminary analysis of coverage distribution14:54:07 21 Aug 201914:55:54 21 Aug 20191 minute 47 seconds
Identifying junction candidates14:55:54 21 Aug 201915:18:10 21 Aug 201922 minutes 16 seconds
Re-alignment to junction candidates15:18:10 21 Aug 201915:20:47 21 Aug 20192 minutes 37 seconds
Resolving best read alignments15:20:47 21 Aug 201915:22:08 21 Aug 20191 minute 21 seconds
Creating BAM files15:22:08 21 Aug 201915:23:44 21 Aug 20191 minute 36 seconds
Tabulating error counts15:23:44 21 Aug 201915:24:32 21 Aug 201948 seconds
Re-calibrating base error rates15:24:32 21 Aug 201915:24:33 21 Aug 20191 second
Examining read alignment evidence15:24:33 21 Aug 201915:34:22 21 Aug 20199 minutes 49 seconds
Polymorphism statistics15:34:22 21 Aug 201915:34:23 21 Aug 20191 second
Output15:34:23 21 Aug 201915:35:04 21 Aug 201941 seconds
Total 52 minutes 19 seconds