Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,668,110 G→A 7.4% intergenic (+85/‑82) prpF → / → prpD aconitate isomerase/2‑methylcitrate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,668,1100GA 7.4% 253.5 / 5.6 90intergenic (+85/‑82)prpF/prpDaconitate isomerase/2‑methylcitrate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (41/41);  new base A (3/4);  total (44/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATGAGCCGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTAGACCTCAACGATCGCCCGGATTACGACCGGGTGCTACAAACCCTGG  >  NC_002947/2667961‑2668252
                                                                                                                                                       |                                                                                                                                              
tCATGAGCCGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTg                                                                                                                                                >  2:535950/1‑150 (MQ=255)
 cATGAGCCGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGa                                                                                                                                               <  2:1557466/150‑1 (MQ=255)
       ccGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCtg                                                                                                                                         <  1:930849/150‑1 (MQ=255)
       ccGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCtg                                                                                                                                         <  1:427398/150‑1 (MQ=255)
        cGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCtgt                                                                                                                                        <  2:1001131/150‑1 (MQ=255)
         gTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCGCCCACA‑GGTA‑ATCACAGGGTTTGAATGCtgtt                                                                                                                                       >  2:1987563/1‑149 (MQ=255)
             tGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGatat                                                                                                                                   >  2:1583052/1‑150 (MQ=255)
             tGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGatat                                                                                                                                   >  1:1694217/1‑150 (MQ=255)
                tCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATccc                                                                                                                                >  2:1409792/1‑150 (MQ=255)
                tCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCGCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCACTCCCACG‑GGGA‑AACAGAGGGTTTGCATGATGGTATATccc                                                                                                                                >  2:621632/1‑150 (MQ=255)
                 cgcgTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCt                                                                                                                               <  2:2033134/150‑1 (MQ=255)
                  gcgTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCtg                                                                                                                                         >  2:257558/1‑139 (MQ=255)
                  gcgTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCtg                                                                                                                                         <  1:257558/139‑1 (MQ=255)
                  gcgTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCtg                                                                                                                              >  2:2281040/1‑150 (MQ=255)
                   cgTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCtgt                                                                                                                             <  1:1578113/150‑1 (MQ=255)
                    gTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCtgtg                                                                                                                            <  1:897789/150‑1 (MQ=255)
                     tGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTgg                                                                                                                           >  1:2363497/1‑150 (MQ=255)
                     tGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTgg                                                                                                                           >  1:448915/1‑150 (MQ=255)
                         gATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGc                                                                                                                       <  1:535950/150‑1 (MQ=255)
                         gATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGc                                                                                                                       <  1:2108791/150‑1 (MQ=255)
                          aTGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGGATATCACAGCATTCAAACCCTGTGAT‑TACCTGTGGGAGCg                                                                                                                      <  2:1812013/150‑1 (MQ=18)
                          aTGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCCCCCACA‑GGGATATCACAGCATTCAAACCCTGTGAT‑TACCTGTGGGAGCg                                                                                                                      >  2:1365839/1‑150 (MQ=18)
                           tGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGGATATCACAGCATTCAAACCCTGTGAT‑TACCTGTGGGAGCgg                                                                                                                     <  2:1170163/150‑1 (MQ=17)
                           tGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGGATATCACAGCATTCAAACCCTGTGAT‑TACCTGTGGGAGCgg                                                                                                                     <  1:41695/150‑1 (MQ=17)
                           gggAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCTCTCGGCTTCAAGAGGGGTCGCTTAGCGGCCCTTCGCGGGCTTGCTCGCTCCNACAGGGNT‑ATCACAGCATTNAAACCCTGTGAT‑TACCTGTGGGAGCgg                                                                                                                     <  1:1365839/149‑1 (MQ=17)
                                  cTGGGTTCGCGTGCCTGGCGATCGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GCTA‑ATCACAGGGTTTGAATGCTGTTATATCCCTGTGGGAGCGGGCAAGcc                                                                                                              >  1:2108204/1‑150 (MQ=255)
                                      gTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGCTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCcgcg                                                                                                          >  1:516631/1‑150 (MQ=255)
                                            gTGCCGGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGCGGGGCCGCTTGGCGGCTCTTCGCGGGCTTGCCCGCCCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGc                                                                                                    <  1:621632/150‑1 (MQ=38)
                                                      aTAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCAcagcag                                                                                          >  1:2104026/1‑150 (MQ=255)
                                                        aGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCggg                                                                                                                    >  1:1654122/1‑122 (MQ=255)
                                                        aGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCggg                                                                                                                    <  2:1654122/122‑1 (MQ=255)
                                                         gCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGccc                                                                                       >  1:1952286/1‑150 (MQ=255)
                                                         gCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGccc                                                                                       <  2:1952286/150‑1 (MQ=255)
                                                                 aGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAAtt                                                                               <  1:2259366/150‑1 (MQ=255)
                                                                  gCACCACTGGCGCTCGGCTTCAAGAGGGGCCTCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCtgt                                                                                                                             >  2:2152307/1‑103 (MQ=255)
                                                                  gCACCACTGGCGCTCGGCTTCAAGAGGGGCCTCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCtgt                                                                                                                             <  1:2152307/103‑1 (MQ=255)
                                                                     ccacTGGCGCTCGGCTTCAAGAGGGGCCGTTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAg                                                                                                       <  1:2203303/122‑1 (MQ=255)
                                                                     ccacTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAg                                                                                                       >  2:2203303/1‑122 (MQ=255)
                                                                     ccacTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGa                                                                           <  2:2227399/150‑1 (MQ=255)
                                                                      cacTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGaa                                                                          >  1:1598340/1‑150 (MQ=255)
                                                                       acTGGCGATCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTTGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAg                                                                         <  1:2152540/150‑1 (MQ=255)
                                                                             gcTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCaa                                                                                                                 >  1:993961/1‑104 (MQ=255)
                                                                             gcTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCaa                                                                                                                 <  2:993961/104‑1 (MQ=255)
                                                                             gcTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCt                                                                   <  2:1694217/150‑1 (MQ=255)
                                                                              cTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTTCCACA‑GGTA‑ATCCCAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTg                                                                  <  1:2121505/150‑1 (MQ=255)
                                                                                    ttCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCgg                                                                                                                     >  2:1079088/1‑93 (MQ=255)
                                                                                    ttCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCgg                                                                                                                     <  1:1079088/93‑1 (MQ=255)
                                                                                    ttCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCCAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATg                                                            >  1:2380449/1‑150 (MQ=255)
                                                                                     tCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCCCA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGa                                                           >  2:555182/1‑150 (MQ=255)
                                                                                     tCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGa                                                           <  1:1583052/150‑1 (MQ=255)
                                                                                     tCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCGCCCCCA‑AGTA‑TTTACAATACTTGAATGCTTTGATATCCCTGTGGGAGCGGGCCAGCCCGCGAAAGGCTGCACAGCAGCCCCGGCCATTCCCAAGGGAGCTGCATATGa                                                           >  2:84829/1‑150 (MQ=25)
                                                                                       aaGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAgc                                                         <  2:448915/150‑1 (MQ=255)
                                                                                        agagGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAgcg                                                        >  1:699152/1‑150 (MQ=255)
                                                                                         gagGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTGGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAgcgc                                                       <  1:1987563/150‑1 (MQ=255)
                                                                                                cGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTa                                                >  2:410177/1‑150 (MQ=255)
                                                                                                   tttGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGaa                                                                                                        >  2:1155759/1‑91 (MQ=255)
                                                                                                   tttGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGaa                                                                                                        <  1:1155759/91‑1 (MQ=255)
                                                                                                   tttGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTAGAc                                             <  2:2104026/150‑1 (MQ=255)
                                                                                                         gCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTAGACCTCAAc                                       <  2:2363497/150‑1 (MQ=255)
                                                                                                            cTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTATGAAGGGAGCTGGATATGAGCGCCAACGTAAACCTCAACGAt                                    >  1:819753/1‑150 (MQ=255)
                                                                                                            cTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATAACAGGGTTTAAATGCTGTAGTAAGCCTGTGAGAGCGGTCAAGCCCTCGGAGGGCTGCACAGCAGCCCCGGCAATTCTGACGGGAGCTGGATATGAGCGCCAACGTAGACCTCAACggt                                    >  2:884133/1‑148 (MQ=37)
                                                                                                                gcgGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTAGACCTCAACGATCGcc                                >  2:1608931/1‑150 (MQ=255)
                                                                                                                 cgGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTAGACCTCAACGATCGccc                               <  1:1608931/150‑1 (MQ=255)
                                                                                                                  gggCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTAGACCTCAACGATCGCCCg                              <  1:410177/150‑1 (MQ=255)
                                                                                                                    gCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAgcgc                                                       <  2:1494800/123‑1 (MQ=255)
                                                                                                                    gCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAgcgc                                                       >  1:1494800/1‑123 (MQ=255)
                                                                                                                    gCTGGCCCGCGCCCACA‑TGTA‑ATCACAGGGTTTGAATGCTGTGATATCTCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGGGCGCCAACGTAGACCTCAACGATCGCCCGGa                            <  1:1068938/150‑1 (MQ=39)
                                                                                                                      ttGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTg                                                                                                  >  1:1600865/1‑78 (MQ=255)
                                                                                                                      ttGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTg                                                                                                  <  2:1600865/78‑1 (MQ=255)
                                                                                                                        gCCCGCTCCCACA‑GGGATATCACAGCATTCAAACCCTGTGAT‑TACCTGTGGGAGCGGGCAAGCCCGCGGAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTAGACCTCAACGATCGCCCGGATTAc                        >  2:453719/1‑150 (MQ=17)
                                                                                                                         cccGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGaa                                                                                                        >  1:699506/1‑69 (MQ=255)
                                                                                                                         cccGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGaa                                                                                                        <  2:699506/69‑1 (MQ=255)
                                                                                                                                ccACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGATGGCCAACGTAGACCTCAACGATCGCCCGGATTACGACCGGGt                >  1:1121009/1‑150 (MQ=255)
                                                                                                                                ccACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTAGACCTCAACGATCGCCCGGATTACGACCGGGt                >  1:1096237/1‑150 (MQ=255)
                                                                                                                                 caca‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAgg                                                                        >  1:2333417/1‑93 (MQ=255)
                                                                                                                                 caca‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAgg                                                                        <  2:2333417/93‑1 (MQ=255)
                                                                                                                                 caca‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTAGACCTCAACGATCGCCCGGATTAc                        >  1:2246127/1‑141 (MQ=255)
                                                                                                                                 caca‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTAGACCTCAACGATCGCCCGGATTAc                        <  2:2246127/141‑1 (MQ=255)
                                                                                                                                 caca‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTAGACCTCAACGATCGCCCGGATTACGACCGGGTg               <  1:2281040/150‑1 (MQ=255)
                                                                                                                                 caca‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCCGCAaagcggc                                                                                         >  2:1445007/1‑73 (MQ=21)
                                                                                                                                 caca‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCCGCAAagcggc                                                                                         <  1:1445007/76‑1 (MQ=21)
                                                                                                                                    a‑GGTA‑ATCTCAGGTTTTCATTGTTGTAATATCTCTGTGGTTGCGGGCATGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTAGACCTCAACGATCGCCCGGATTACGACCGGGTGCTa            <  1:84829/150‑1 (MQ=255)
                                                                                                                                        tA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTAGACCTCAACGATCGCCCGGATTACGACCGGGTGCTACaa         >  1:468753/1‑150 (MQ=255)
                                                                                                                                              acaGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTAGACCTCAACGATCGCCCGGATTACGACCGGGTGCTACAAACCCt    <  2:1096237/150‑1 (MQ=255)
                                                                                                                                                atggTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGATCGCCAACGTAGACCTCAACGATCGCCCGGATTACGACCGGGTGCTACAAACCCTgg  >  2:1349886/3‑150 (MQ=255)
                                                                                                                                                aGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGCGTCGGGATATGAGCGCCAACGTAGACCTCAACGATCGCCCGGATTACGACCGGGTGCTACAAACCCTgg  >  1:151093/1‑150 (MQ=255)
                                                                                                                                                aGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGAGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTAGACCTCAACGATCGCCCGGATTACGACCGGGTGCTACAAACCCTgg  >  1:465552/1‑150 (MQ=255)
                                                                                                                                                   gTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGaa                                                                          >  2:643896/1‑75 (MQ=255)
                                                                                                                                                   gTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGaa                                                                          <  1:643896/75‑1 (MQ=255)
                                                                                                                                                   gTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTAGa                                              >  2:2326904/1‑103 (MQ=255)
                                                                                                                                                   gTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTAGa                                              <  1:2326904/103‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                              
TCATGAGCCGTAGTGCTCGCGTGCTGATGGAGGGCTGGGTTCGCGTGCCTGGCGATAGCTTCTAAAGCACCACTGGCGCTCGGCTTCAAGAGGGGCCGCTTTGCGGCCCTTCGCGGGCTTGCCCGCTCCCACA‑GGTA‑ATCACAGGGTTTGAATGCTGTGATATCCCTGTGGGAGCGGGCAAGCCCGCGAAGGGCTGCACAGCAGCCCCGGCAATTCTGAAGGGAGCTGGATATGAGCGCCAACGTAGACCTCAACGATCGCCCGGATTACGACCGGGTGCTACAAACCCTGG  >  NC_002947/2667961‑2668252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑