| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,852,280 | C→T | H442Y (CAC→TAC) | relA → | ATP:GTP 3'‑pyrophosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,852,280 | 0 | C | T | 97.3% | 116.9 / ‑6.2 | 37 | H442Y (CAC→TAC) | relA | ATP:GTP 3'‑pyrophosphotransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (20/16); minor base G (0/1); total (20/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
ACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAA > NC_002947/1852137‑1852416 | aCGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCTGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCg > 1:695095/1‑150 (MQ=255) cGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCga < 1:688088/150‑1 (MQ=255) aTCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGc < 1:555064/150‑1 (MQ=255) aTCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGc > 1:987484/1‑150 (MQ=255) ggcctggccGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCgcggc < 1:142584/150‑1 (MQ=255) gccGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCGAAg > 1:127253/1‑150 (MQ=255) ttGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCAACCGCGTGTACACAGATTTCTGGCCCAACTGCCGCGGCGCGACGATCAACGGc > 2:379753/1‑150 (MQ=255) gACAGCGAGCCTGACCGGGTTTATGTGTTCACCCCCCACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTTGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTg < 2:1008774/150‑1 (MQ=255) aCATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGCCCGTATCGTGc > 1:237154/1‑150 (MQ=255) tCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGc > 1:673457/1‑150 (MQ=255) cGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCt < 2:127253/150‑1 (MQ=255) gCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCCCGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCCAAGATCAACGGACGTCTCGAGCCCCCGaa > 2:189583/1‑150 (MQ=255) ccTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAAc < 2:987484/150‑1 (MQ=255) ccTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAAc > 1:1028101/1‑150 (MQ=255) aCCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACa < 2:927628/150‑1 (MQ=255) cGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGc > 2:1034019/1‑150 (MQ=255) cGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGc > 1:224964/1‑150 (MQ=255) ggTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACAcc < 1:559059/85‑1 (MQ=255) ggTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACAcc > 2:559059/1‑85 (MQ=255) ttATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGct < 2:673457/150‑2 (MQ=255) gtTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTgg > 1:950920/1‑150 (MQ=255) gtTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTgg > 2:366893/1‑150 (MQ=255) cACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGa > 1:607365/1‑150 (MQ=255) accaccGACGGCTACGCGATCGACCTGCCCAAAGGCGCCACGCAATCGGCTCTCGCCTACCGCGTGTCCACCCAGATCGGCCACAACGGCCGCGGCGTGAAGATCCACCGCCGTCTCCTGCCGCCGCACTACAGCCTGCAGACTGGCGAg < 2:66849/150‑1 (MQ=255) aCCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGgcgc < 2:16596/98‑1 (MQ=255) aCCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGgcgc > 1:16596/1‑98 (MQ=255) aCCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAg < 1:1034019/150‑1 (MQ=255) gACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTg < 2:826633/150‑1 (MQ=255) cATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCAc > 2:842314/1‑150 (MQ=255) aTCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGCCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCAcc < 2:237154/150‑1 (MQ=255) gACCTGCCCAAAGGGGCCACGCTATTGGGGTTGACCGACGTCGTGTACACTGAGAGCGGCCACAACTGCCGCGGCGCGAAGATCAACGTCCGTATCGTGCCGCTGAAATACACCCCACCGACTTGCGAGGATGTATAGATTATCACCagc < 2:358800/150‑1 (MQ=255) ccTGCCCAAAGGCGCCCCGCAACTGGGTTTAGCCTACGGGGGGGAGACCGTGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCagcaa < 2:695095/150‑1 (MQ=255) cccAAAGGGGCCACACCATTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGACGGGAGACGACCAACGCGCGTATCGAGCCACCGACCGACGCGCTGCAGACAGGCCAAAAATTGGCGACCATCACCagcaagcaa > 2:122723/1‑149 (MQ=255) ccacgccaTTGGACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCa > 1:642181/1‑150 (MQ=255) caTTGGACTTCGCCTACCGCAAGTACACCGAGATCGGCCACAACTGCCGCTGGAAAAAGATCAACAGCCATATCGTGACGCTCAACTACAGACTGCAGACTACCGAGCACGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTg > 2:421046/1‑150 (MQ=255) gACTTCGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTgg > 1:1102626/1‑150 (MQ=255) cGCCTACCGCGTGTACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGaa < 1:366893/150‑1 (MQ=255) | ACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAA > NC_002947/1852137‑1852416 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |