Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	643,952	C→G	M19I (ATG→ATC)	acoB ←	acetoin:2,6‑dichlorophenolindophenol oxidoreductase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	643,952	0	C	G	100.0%	179.0 / NA	53	M19I (ATG→ATC)	acoB	acetoin:2,6‑dichlorophenolindophenol oxidoreductase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (23/30);  total (23/30)

CGCGTCAAGCACGCGGCCGGGGAATTGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGCATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTG  >  NC_002947/643811‑644093                                                                                                                                              |                                                                                                                                              cgcgTCAAGCACGGGGCCGGGGAATTGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTg                                                                                                                                       <  2:244417/150‑1 (MQ=255)         gCACGCGGCCGGGGAATTGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAggg                                                                                                                               <  2:812751/150‑1 (MQ=255)         gCACGCGGCCGGGGAATTGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAggg                                                                                                                               >  1:632906/1‑150 (MQ=255)          cACGCGGCCGGGGAATTGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGc                                                                                                                              <  2:504290/150‑1 (MQ=255)              cgGCCGGGGAAGTCGTGATACAGGACTTTGGTCACGCCCAACACGCCACACCAGGCGCCATACTCGCCGGGCGCACCGGCACCGCCGGCGGCGTCTTCGCTAATGATGAATTCTGTGCTGTCTCGGCGGATTTCCTGGGCCAGGGCCTCg                                                                                                                          <  2:138642/150‑1 (MQ=255)                            gTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCctgctg                                                                                                            <  1:666878/150‑1 (MQ=255)                             tGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTgg                                                                                                           >  2:403697/1‑150 (MQ=255)                                 aCAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATAAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGc                                                                                                       <  1:350701/150‑1 (MQ=255)                                    ggCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGa                                                                                                    >  1:127754/1‑150 (MQ=255)                                      ccTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGAtc                                                                                                  >  1:134590/1‑150 (MQ=255)                                         ttGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGAtcttt                                                                                               <  2:127754/150‑1 (MQ=255)                                            gTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGAtctttctt                                                                                            <  2:596895/150‑1 (MQ=255)                                             tCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCct                                                                                                                >  2:517191/1‑129 (MQ=255)                                             tCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCct                                                                                                                <  1:517191/129‑1 (MQ=255)                                               aCGCCCAACACGCCACCGCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGACAGGTCCTCGTTGATGGCCTGCGTGCAGCTGATCTTTCTTGcc                                                                                         >  2:243280/1‑150 (MQ=255)                                               aCGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGcc                                                                                         >  2:915243/1‑150 (MQ=255)                                               aCGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGcc                                                                                         >  1:325642/1‑150 (MQ=255)                                                   ccAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCAtgg                                                                                     <  2:244793/150‑1 (MQ=255)                                                    cAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCAtggt                                                                                    >  1:630158/1‑150 (MQ=255)                                                    cAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAAGGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCAtggt                                                                                    >  2:660195/1‑150 (MQ=255)                                                     aaCACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCAtggtg                                                                                   >  2:322623/1‑150 (MQ=255)                                                      acacGCCACCCCAGGCGTCATCCGCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCAtggtgg                                                                                  <  2:20859/150‑1 (MQ=255)                                                           ccACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTtctc                                                                             <  2:527345/150‑1 (MQ=255)                                                            cACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTcc                                                                            >  1:625651/1‑150 (MQ=255)                                                              ccccAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCtg                                                                          <  1:915243/150‑1 (MQ=255)                                                                ccAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCtgtt                                                                        <  2:630158/150‑1 (MQ=255)                                                                      gTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCtt                                                                  >  1:11924/1‑150 (MQ=255)                                                                      gTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATAATGTGCTCTCGGGGCGGATTTCCTGGGCCCGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCtt                                                                  <  1:893291/150‑1 (MQ=255)                                                                           ccTCGCCGGGCTCACCGGCACCGCCGTCGACGTCTTCGCCAATGATGAATACTCTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTTATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAAt                                                             <  1:874525/150‑1 (MQ=255)                                                                           ccTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAAt                                                             <  2:325642/150‑1 (MQ=255)                                                                                ccGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCcgcg                                                        <  2:632906/150‑1 (MQ=255)                                                                                       caccggcaccgCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCATCGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAgg                                                 <  1:886814/150‑1 (MQ=255)                                                                                        accgggacggCCGGCGACGTCTTCGCCGATGATGAATACTGTGCTGTCGCGGAGGATTACCAGGGCCGGGGCCCCGTTGGTGAACTCGTGGCAGCCGCTCTTTATTGCCATGGTGGCTCCCCTGTTGTTATTGTGATCCGCGCATCAggg                                                >  2:125706/1‑150 (MQ=255)                                                                                           ggcaccgCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTa                                             >  1:307982/1‑150 (MQ=255)                                                                                                    ggCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAAACCGCGGCTCAGGGGTAGGCGACGTa                                    <  2:11924/150‑1 (MQ=255)                                                                                                           tCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGGCCGCGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCg                             >  1:23634/1‑150 (MQ=255)                                                                                                             ttCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAg                                            <  2:745136/133‑1 (MQ=255)                                                                                                             ttCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAg                                            >  1:745136/1‑133 (MQ=255)                                                                                                                gCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCtgt                                                                         <  2:915175/101‑1 (MQ=255)                                                                                                                gCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCtgt                                                                         >  1:915175/1‑101 (MQ=255)                                                                                                                  cAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCa                      <  1:322623/150‑1 (MQ=255)                                                                                                                   aaTGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGt                           <  1:668166/144‑1 (MQ=255)                                                                                                                   aaTGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGt                           >  2:668166/1‑144 (MQ=255)                                                                                                                       atgaATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGc                       >  1:512925/1‑144 (MQ=255)                                                                                                                       atgaATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGc                       <  2:512925/144‑1 (MQ=255)                                                                                                                           aTACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTcggcgg             <  1:403697/150‑1 (MQ=255)                                                                                                                              cTGTGCTGTCGCGGCGGATTTCCTGGGCAAGGGGGTCAGCTAGGTTCTGCTGTTAGCTGATCTTTCTTGCCATGGGGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCt          <  1:660195/150‑1 (MQ=255)                                                                                                                               tgtgCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCtg         >  2:883379/1‑150 (MQ=255)                                                                                                                               tgtgCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCtg         >  1:513431/1‑150 (MQ=255)                                                                                                                                 tgCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGAc                                       <  1:861799/118‑1 (MQ=255)                                                                                                                                 tgCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGAc                                       >  2:861799/1‑118 (MQ=255)                                                                                                                                      tCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTg  <  2:625651/150‑1 (MQ=255)                                                                                                                                      tCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTg  <  1:88757/150‑1 (MQ=255)                                                                                                                                              |                                                                                                                                              CGCGTCAAGCACGCGGCCGGGGAATTGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGCATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTG  >  NC_002947/643811‑644093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑