Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,851,994	G→T	E346D (GAG→GAT)	relA →	ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,851,994	0	G	T	100.0%	135.0 / NA	40	E346D (GAG→GAT)	relA	ATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/16);  total (24/16)

CTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCAC  >  NC_002947/1851853‑1852138                                                                                                                                              |                                                                                                                                                 cTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCACTCGTTTCACACTGCGGTAATCGGCCCCGAGGGCGAGGTGCTGGATGTGCAGAt                                                                                                                                          <  1:250780/150‑1 (MQ=255)            cgcTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACg                                                                                                                               <  1:892307/150‑1 (MQ=255)                           acacCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAaga                                                                                                                  >  2:320584/1‑148 (MQ=255)                           acacCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAaga                                                                                                                  <  1:320584/148‑1 (MQ=255)                           acacCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAgg                                                                                                                >  1:690839/1‑150 (MQ=255)                           acacCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAgg                                                                                                                >  1:501087/1‑150 (MQ=255)                           acacCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAgg                                                                                                                >  1:650115/1‑150 (MQ=255)                                    ggcggcATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTg                                                                                                       >  2:439678/1‑150 (MQ=255)                                      cggcATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGc                                                                                                     >  2:809075/1‑150 (MQ=255)                                             ttCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATgcg                                                                                              >  2:333674/1‑150 (MQ=255)                                              tCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATgcgc                                                                                             >  1:640888/1‑150 (MQ=255)                                                 cAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCa                                                                                          <  2:640888/150‑1 (MQ=255)                                                 cAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCa                                                                                          >  2:570994/1‑150 (MQ=255)                                                 cAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCa                                                                                          >  1:855420/1‑150 (MQ=255)                                                      aGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGgc                                                                                     <  1:570994/150‑1 (MQ=255)                                                       gTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTg                                                                                                       <  1:354585/131‑1 (MQ=255)                                                       gTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGAGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTg                                                                                                       >  2:354585/1‑131 (MQ=255)                                                              gacTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAg                                                                             <  1:877850/150‑1 (MQ=255)                                                                 tACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGc                                                                          >  1:855589/1‑150 (MQ=255)                                                                    aTCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAcc                                                                       >  2:12538/1‑150 (MQ=255)                                                                         cAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGt                                                                  <  2:855420/150‑1 (MQ=255)                                                                              ccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACaa                                                                              <  2:143523/133‑1 (MQ=255)                                                                              ccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACaa                                                                              >  1:143523/1‑133 (MQ=255)                                                                                 aGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCa                                                          >  1:68615/1‑150 (MQ=255)                                                                                         ggCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTAt                                                                                                 >  1:573729/1‑103 (MQ=255)                                                                                         ggCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTAt                                                                                                 <  2:573729/103‑1 (MQ=255)                                                                                                    gTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACgaaga                                       >  1:499387/1‑150 (MQ=255)                                                                                                               cGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTc                              >  1:211237/1‑148 (MQ=255)                                                                                                               cGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTc                              <  2:211237/148‑1 (MQ=255)                                                                                                                ggTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTg                                                                                       <  1:897619/90‑1 (MQ=255)                                                                                                                ggTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTg                                                                                       >  2:897619/1‑90 (MQ=255)                                                                                                                       ggCCCCGAGGGCAAGGTGCTGGATGTGCCGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGt                    >  1:483765/1‑150 (MQ=255)                                                                                                                          cccGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAg                 <  2:690839/150‑1 (MQ=255)                                                                                                                           ccGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAgg                >  2:772464/1‑150 (MQ=255)                                                                                                                            cGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCaa                                                   <  1:69359/114‑1 (MQ=255)                                                                                                                            cGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCaa                                                   >  2:69359/1‑114 (MQ=255)                                                                                                                                    aGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGt       <  1:772464/150‑1 (MQ=255)                                                                                                                                    aGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGt       <  2:650115/150‑1 (MQ=255)                                                                                                                                     ggTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTg      >  1:471171/1‑150 (MQ=255)                                                                                                                                         cTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCAc  >  1:691930/1‑150 (MQ=255)                                                                                                                                              |                                                                                                                                                 CTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCAC  >  NC_002947/1851853‑1852138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑