Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	643,952	C→G	M19I (ATG→ATC)	acoB ←	acetoin:2,6‑dichlorophenolindophenol oxidoreductase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	643,952	0	C	G	100.0%	186.4 / NA	56	M19I (ATG→ATC)	acoB	acetoin:2,6‑dichlorophenolindophenol oxidoreductase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (27/29);  total (27/29)

CAAGCACGCGGCCGGGGAATTGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGCATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGG  >  NC_002947/643816‑644095                                                                                                                                          |                                                                                                                                                cAAGCACGCGGCCGGGGAATTGGTGATACAGGCCTTTGGTCACGCCCCACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCa                                                                                                                                    >  2:514728/1‑150 (MQ=255)  aaGCACGCGGCCGGGGAATTGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAg                                                                                                                                   <  1:383315/150‑1 (MQ=255)        gcgGCCGGGGAATTGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTc                                                                                                                             >  1:194969/1‑150 (MQ=255)          ggCCGGGGAATTGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGt                                                                                                                           <  2:194969/150‑1 (MQ=255)             cGGGGAATTGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACAGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGa                                                                                                                        >  1:541244/1‑150 (MQ=255)              ggggAATTGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGAt                                                                                                                       <  1:602695/150‑1 (MQ=255)                  aaTTGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGCTGACAACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGcc                                                                                                                   >  1:451911/1‑150 (MQ=255)                    ttGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCctg                                                                                                                 >  1:252647/1‑150 (MQ=255)                      ggTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCctgct                                                                                                               >  2:40795/1‑150 (MQ=255)                        tGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTgg                                                                                                             <  2:650591/150‑1 (MQ=255)                         gATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCAGCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGt                                                                                                            <  1:185102/150‑1 (MQ=255)                             cAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCt                                                                                                        <  1:771616/150‑1 (MQ=255)                              aGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTg                                                                                                       >  1:334095/1‑150 (MQ=255)                               ggCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGa                                                                                                      <  2:541244/150‑1 (MQ=255)                                   tttGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGAtctt                                                                                                  >  1:343746/1‑150 (MQ=255)                                   tttGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGAtctt                                                                                                  >  2:568343/1‑150 (MQ=255)                                               cAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCAtggt                                                                                      >  2:713543/1‑150 (MQ=255)                                                aaCACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGc                                                                                            >  1:8684/1‑143 (MQ=255)                                                aaCACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGc                                                                                            <  2:8684/143‑1 (MQ=255)                                                aaCACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑AACCTCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCAtggtg                                                                                     >  1:786659/1‑150 (MQ=255)                                                   acGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGtt                                                                                  >  1:420221/1‑150 (MQ=255)                                                     gCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGc                                                                                                         >  1:458503/1‑125 (MQ=255)                                                     gCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGc                                                                                                         <  2:458503/125‑1 (MQ=255)                                                      ccACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTtctc                                                                               <  2:343746/150‑1 (MQ=255)                                                         ccccAGGCGTCATCCTCGCCGGGCGCACCGGCCGCAGCCGCCTGCAGC‑GCGCCGATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCGTATGTTTCTTGCCATGGTGGTTCTCCtg                                                                            >  1:140913/1‑150 (MQ=255)                                                           ccAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCtgtt                                                                          <  2:420221/150‑1 (MQ=255)                                                                 gTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCtt                                                                    <  2:554135/150‑1 (MQ=255)                                                                 gTCATCCTCCCCGGGCGTACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGTCCTCGTTCATGGCCTGCTGGTAGCTGATCTTTCTTGCCATTGTGGTTCTCCTGTTGTTCtt                                                                    <  2:35255/150‑1 (MQ=255)                                                                    aTCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTTCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCGTGTTGTTCTTGTa                                                                 >  2:392650/1‑150 (MQ=255)                                                                        tCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATcc                                                             >  1:759885/1‑150 (MQ=255)                                                                              ggCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCCTGGTGGTTCTTCTGTTGTTCTTGTAATCCGCGGCt                                                       >  2:64183/1‑150 (MQ=255)                                                                                 gcaccgg‑caccgCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAg                                                    <  2:451911/150‑1 (MQ=255)                                                                                   accgg‑caccgCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCgggg                                                  >  2:106222/1‑150 (MQ=255)                                                                                       g‑caccgCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGAtctttc                                                                                                >  2:409899/1‑100 (MQ=255)                                                                                       g‑caccgCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGAtctttc                                                                                                <  1:409899/100‑1 (MQ=255)                                                                                             gccgGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTc                                                                                                                             >  2:449782/1‑66 (MQ=255)                                                                                             gccgGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTc                                                                                                                             <  1:449782/66‑1 (MQ=255)                                                                                                 gCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAg                                     <  1:713543/150‑1 (MQ=255)                                                                                                  cGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGa                                    <  2:252647/150‑1 (MQ=255)                                                                                                  cGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGa                                    <  1:447011/150‑1 (MQ=255)                                                                                                   gACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGAc                                   <  1:792027/150‑1 (MQ=255)                                                                                                              cAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTGTATGGCCAGCAGGTAGCGGATATATCTTGACTTCCAGGTTCGCCGGGTTTTCTTTTAATCCGCGGCTCAGGGGTAGGCGACGATGACTTCGGTGAGCa                        >  1:385111/1‑150 (MQ=255)                                                                                                                atgatgAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAg                                                                                                                                   <  1:783053/41‑1 (MQ=255)                                                                                                                atgatgAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAg                                                                                                                                   >  2:783053/1‑41 (MQ=255)                                                                                                                atgatgAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAgg                      <  2:759885/150‑1 (MQ=255)                                                                                                                     gaATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTcggc                 >  1:179572/1‑150 (MQ=255)                                                                                                                      aaTTCTGTGCTTTCGGGGCGGATTTCCTGGGGCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGGCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTcggcg                <  2:746284/150‑1 (MQ=255)                                                                                                                         aCTGTGCTGTCGCGGCGGATTTCCTGGGCCAGTGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGc             <  2:786659/150‑1 (MQ=255)                                                                                                                           tgtgCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCg                                           >  1:187093/1‑118 (MQ=255)                                                                                                                           tgtgCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCg                                           <  2:187093/118‑1 (MQ=255)                                                                                                                            ttgCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGGTCTTGGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCtgt          <  1:49878/149‑1 (MQ=255)                                                                                                                              gCTGTCGCGGCCGATCTGCGAGTCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTgg        <  2:624170/150‑1 (MQ=255)                                                                                                                                  tCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGCCGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTg    <  2:576317/150‑1 (MQ=255)                                                                                                                                    gcggcgGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTggg  >  1:633202/1‑150 (MQ=255)                                                                                                                                      ggcgGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCtgt          <  2:617305/140‑1 (MQ=255)                                                                                                                                      ggcgGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCtgt          >  1:617305/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                                CAAGCACGCGGCCGGGGAATTGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGG‑CACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGCATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGG  >  NC_002947/643816‑644095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑