Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,851,994	G→T	E346D (GAG→GAT)	relA →	ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,851,994	0	G	T	100.0%	116.6 / NA	36	E346D (GAG→GAT)	relA	ATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (20/16);  total (20/16)

CCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACG  >  NC_002947/1851847‑1852139                                                                                                                                                    |                                                                                                                                                  ccGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGt                                                                                                                                                 <  1:822060/150‑1 (MQ=255)        tGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATc                                                                                                                                          >  2:215724/1‑150 (MQ=255)                                      ttGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGAGCCGTACCCACGGCATGCACGAAGAGGCCGaa                                                                                                            >  1:204605/1‑150 (MQ=255)                                         tgGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGAGCCGTACCCACGGCATGCACGAAGAGGCCGAACtt                                                                                                         <  2:204605/150‑1 (MQ=255)                                            cggcATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGc                                                                                                      <  2:299343/150‑1 (MQ=255)                                              gcATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAtgtg                                                                                                                                                >  2:231711/1‑106 (MQ=255)                                              gcATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAtgtg                                                                                                                                                <  1:231711/106‑1 (MQ=255)                                                    tCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATgcgc                                                                                              <  1:215724/150‑1 (MQ=255)                                                      ccAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGccc                                                                                            >  1:1231075/1‑150 (MQ=255)                                                      ccAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGccc                                                                                            >  1:561154/1‑150 (MQ=255)                                                       cAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCa                                                                                           >  2:1125891/1‑150 (MQ=255)                                                                 gacgacTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCGTGGCGTGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTAc                                                                                 >  1:747156/1‑150 (MQ=255)                                                                         cATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAgg                                                                             >  2:271495/1‑146 (MQ=255)                                                                         cATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAgg                                                                             <  1:271495/146‑1 (MQ=255)                                                                         cATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAc                                                                         <  2:476457/150‑1 (MQ=255)                                                                           tCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCTGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCg                                                                       >  2:323947/1‑150 (MQ=255)                                                                            cGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGTAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGGTACAAGGGCACCGa                                                                      >  1:609455/1‑150 (MQ=255)                                                                               cAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGt                                                                   <  2:1231075/150‑1 (MQ=255)                                                                                   cccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGTTGGATGTGCAGATCCGTTAGTAGGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGAACACTAGACCTATAAGCGCAACGACGTCAAg                                                               >  1:386419/1‑150 (MQ=255)                                                                                   cccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTgg                                                                                       >  1:733879/1‑126 (MQ=255)                                                                                   cccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTgg                                                                                       <  2:733879/126‑1 (MQ=255)                                                                                    ccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAc                                                                         >  2:491714/1‑139 (MQ=255)                                                                                    ccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAc                                                                         <  1:491714/139‑1 (MQ=255)                                                                                     cAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGcc                                                             <  2:578899/150‑1 (MQ=255)                                                                                      aaGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGc                                                                                                      >  2:1021968/1‑108 (MQ=255)                                                                                      aaGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGc                                                                                                      <  1:1021968/108‑1 (MQ=255)                                                                                      aaGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGccc                                                            <  1:419798/150‑1 (MQ=255)                                                                                      aaGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGccc                                                            >  2:672748/1‑150 (MQ=255)                                                                                               ggCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAAc                                                   >  2:7246/1‑150 (MQ=255)                                                                                                             gCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGaaaa                                     <  1:323947/150‑1 (MQ=255)                                                                                                                 acTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTAc                                                                                 <  1:597536/102‑1 (MQ=255)                                                                                                                 acTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTAc                                                                                 >  2:597536/1‑102 (MQ=255)                                                                                                                   tGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGc                                                         >  1:501248/1‑124 (MQ=255)                                                                                                                   tGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGc                                                         <  2:501248/124‑1 (MQ=255)                                                                                                                   tGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTc                               >  2:909684/1‑150 (MQ=255)                                                                                                                            cGGCCCGGTGGGTAAAGGGCTGGATGTGCACATCCGTACCGCCGGCTTGCACGATGAGCCCTAGCGCGATGTGTGCGCCGACTGGCGGTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCg                      >  2:38449/1‑150 (MQ=255)                                                                                                                                        cAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGa          <  1:909684/150‑1 (MQ=255)                                                                                                                                                tGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGATGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACg  >  1:626340/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACG  >  NC_002947/1851847‑1852139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑