Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,859,573 G→T 7.5% intergenic (‑123/+33) PP_1662 ← / ← PP_1663 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,859,5730GT 7.5% 212.5 / 4.7 67intergenic (‑123/+33)PP_1662/PP_1663hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (33/29);  new base T (2/3);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01

TGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGTCTCTACCTGGCGCAGTTGCACCAGCAGGTAGTCCCAG  >  NC_002947/1859433‑1859714
                                                                                                                                            |                                                                                                                                             
tGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCCCACTGGGCGGGGCtt                                                                                                                                      >  2:1560267/1‑150 (MQ=255)
   gACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAg                                                                                                                                   >  1:157164/1‑150 (MQ=255)
   gACAGTCTGTCGCAGGACTGATTTATCGAAGTTGTGAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAGAAGCCCCGCCCAGTGCGGGGCttttc                                                                                                                                   <  2:419354/150‑4 (MQ=255)
     cAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCGCGCTGTGGGCGGGGCTTCAGGt                                                                                                                                 >  2:931903/1‑150 (MQ=255)
          tgtCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTgc                                                                                                                            <  1:62728/150‑1 (MQ=255)
           gtCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTgca                                                                                                                           <  2:1942394/150‑1 (MQ=255)
           gtCGCACGGCTTCTGTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTgca                                                                                                                           <  1:273115/150‑1 (MQ=255)
            tCGCACGTCTTCTTGATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTgcag                                                                                                                          >  2:1044045/1‑150 (MQ=255)
                     ttcttTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACACGCCAGGTGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTgcgc                                                                                                                 >  2:1441366/1‑150 (MQ=255)
                       cttTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGa                                                                                                               >  1:1687676/1‑150 (MQ=255)
                       cttTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGa                                                                                                               >  2:1029167/1‑150 (MQ=255)
                        tttATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAGAAGCCCCGCCCAGTGCGGGGCttttctt                                                                                                                                 >  2:205734/1‑126 (MQ=255)
                        tttATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAGAAGCCCCGCCCAGTGCGGGGCttttctt                                                                                                                                 <  1:205734/131‑6 (MQ=255)
                           ataGAGTTTGTTAGCAGCCTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAg                                                                                                           >  2:994577/1‑150 (MQ=255)
                             agagTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGtt                                                                                                         <  1:1896837/150‑1 (MQ=255)
                               agTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGc                                                                                                       >  1:424043/1‑150 (MQ=255)
                               agTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGc                                                                                                       <  1:1044045/150‑1 (MQ=255)
                                                agtaagtaAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCa                                                                                      >  1:634797/1‑150 (MQ=255)
                                                    agtaaAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGa                                                                                  >  2:159942/1‑150 (MQ=255)
                                                    agtaaAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGa                                                                                  <  1:597262/150‑1 (MQ=255)
                                                    agtaaAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGAGCTTCAGGTATTGCAGGCAGTCCGCGAACAGCTGCCCTTCACCGGCCTGCCATCGa                                                                                  <  2:998017/150‑1 (MQ=255)
                                                      taaAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCCCAGTGCGGGGCttttctt                                                                                                                                 <  1:224009/101‑6 (MQ=38)
                                                      taaAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCCCAGTGCGGGGCttttctt                                                                                                                                 >  2:224009/1‑96 (MQ=38)
                                                      taaAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGAcc                                                                                <  2:157164/150‑1 (MQ=255)
                                                        aaaCAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGt                                                                              <  1:1793585/150‑1 (MQ=255)
                                                         aaCAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTg                                                                             <  2:1687676/150‑1 (MQ=255)
                                                            cttcATGAAACTTGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGAt                                                                                                              <  2:1645619/111‑1 (MQ=255)
                                                            cttcATGAAACTTGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGAt                                                                                                              >  1:1645619/4‑114 (MQ=255)
                                                                  gAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTgc                                                                    >  1:845883/1‑150 (MQ=255)
                                                                    aaCAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTa                                                                                                                                <  2:885596/88‑1 (MQ=255)
                                                                    aaCAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTa                                                                                                                                >  1:885596/1‑88 (MQ=255)
                                                                    aaCAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTgcag                                                                  >  2:1700689/1‑150 (MQ=255)
                                                                         gTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCAt                                                                                     <  1:1043651/126‑1 (MQ=255)
                                                                         gTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCAt                                                                                     >  2:1043651/1‑126 (MQ=255)
                                                                             cATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATcac                                                         >  1:1714027/1‑150 (MQ=255)
                                                                                gAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATa                                                      <  2:634797/150‑1 (MQ=255)
                                                                                     tCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCt                                                 >  1:1798072/1‑150 (MQ=255)
                                                                                      cGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCtt                                                <  1:964274/150‑1 (MQ=255)
                                                                                       gAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTg                                               >  1:837590/1‑150 (MQ=255)
                                                                                       gAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCAACGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTg                                               <  1:931903/150‑1 (MQ=255)
                                                                                             aTGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCg                                         <  1:650005/150‑1 (MQ=255)
                                                                                             aTGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCg                                         >  1:1175187/1‑150 (MQ=255)
                                                                                              tgctgcCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCgg                                        <  2:845883/150‑1 (MQ=255)
                                                                                              tgctgcCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCgg                                        <  1:159942/150‑1 (MQ=255)
                                                                                                 tgcCTGGGTGGGCTTTTTCGCAGCAAAGAAAGGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGtct                                     >  1:153151/1‑150 (MQ=255)
                                                                                                 tgcCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGtct                                     >  2:1205753/1‑150 (MQ=255)
                                                                                                       ggtgggCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGTCTCTACCt                               <  1:1169315/150‑1 (MQ=255)
                                                                                                         tgggCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCAcc                                                                                               <  2:882065/84‑1 (MQ=255)
                                                                                                         tgggCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCAcc                                                                                               >  1:882065/1‑84 (MQ=255)
                                                                                                             cTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGTCTCTACCTGGCGCa                         >  1:285812/1‑150 (MQ=255)
                                                                                                               ttttCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGTCTCTACCTGGCGCAGt                       <  1:1788528/150‑1 (MQ=255)
                                                                                                                tttCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCCGCTTGCCATCGACCGTGTCATCCNGCANCACGATCACATACTCCTTGCCATCGGTCTCTACCTGGCGCAGtt                      <  1:1560267/150‑1 (MQ=255)
                                                                                                                 ttCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGTCTCTACCTGGCGCAGTTg                     >  2:1453749/1‑150 (MQ=255)
                                                                                                                      agcaAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCtt                                                                                          >  2:1912663/1‑76 (MQ=255)
                                                                                                                      agcaAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCtt                                                                                          <  1:1912663/76‑1 (MQ=255)
                                                                                                                         aaaGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATcc                                                                       >  2:696338/1‑92 (MQ=255)
                                                                                                                         aaaGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGTCTCTACCTGGCGCAGTTGCACcagca             >  2:1607271/1‑150 (MQ=255)
                                                                                                                         aaaGAAAAGCCCCGCACTGGGCGGGGCGTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGGGCCATcc                                                                       <  1:696338/92‑1 (MQ=255)
                                                                                                                          aaGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGtct                                     <  1:1504573/125‑1 (MQ=255)
                                                                                                                          aaGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGtct                                     >  2:1504573/1‑125 (MQ=255)
                                                                                                                          aaGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGAACACATACTCCTTGCCATCGGTCTCTACCTGGCGCAGTTGCACcagcag            >  2:830499/1‑150 (MQ=255)
                                                                                                                                aGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGTCTCTACCTGGCGCAGTTGCACCAGCAGGTAGTc      <  2:1798072/150‑1 (MQ=255)
                                                                                                                                   cccGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGcc                                                                                       >  2:1420163/1‑66 (MQ=255)
                                                                                                                                   cccGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGcc                                                                                       <  1:1420163/66‑1 (MQ=255)
                                                                                                                                   cccGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTgca                                                                   >  1:263214/1‑86 (MQ=255)
                                                                                                                                   cccGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTgca                                                                   <  2:263214/86‑1 (MQ=255)
                                                                                                                                    ccGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGTCTCTACCTGGCGCAGTTGCACCAGCAGGTAGTCCCAg  >  1:293900/1‑150 (MQ=255)
                                                                                                                                            |                                                                                                                                             
TGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGTCTCTACCTGGCGCAGTTGCACCAGCAGGTAGTCCCAG  >  NC_002947/1859433‑1859714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑