Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 546,893 G→T G81C (GGC→TGC)  rpsG → 30S ribosomal protein S7

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947546,8930GT98.7% 481.3 / ‑4.5 149G81C (GGC→TGC) rpsG30S ribosomal protein S7
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (79/68);  total (81/68)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

AAAGCGGCAAGAAGGCCGTAGCCGAGCGCATCGTTTACGGTGCCCTGGATACCGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCGGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCGAGAAGTCGATGGCTCTGCGTCTGGCCGGCGAACTGCTGGATGCTGCCGAA  >  NC_002947/546747‑547039
                                                                                                                                                  |                                                                                                                                                   
aaaGCGGCAAGAAGGCCGTAGTCGAGCGCATCGTTTACGGTGCCCTGGATACCGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCATCGTGTctgcg                                                                                                                                                  >  2:2470285/1‑150 (MQ=255)
 aaGCGGCAAGAAGGCCGTAGCCGAGCGCATCGTTTACGGTGCCCTGGATACCGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTctgcgg                                                                                                                                                 >  1:3380180/1‑150 (MQ=255)
      gCAAGAAGGCCGTAGCCGAGCGCATCGTTTACGGTGCCCTGGATACCGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCTGTGCCa                                                                                                                                            <  1:1624362/150‑1 (MQ=255)
      gCAAGAAGGCCGTAGCCGAGCGCATCGTTTACGGTGCCCTGGATACCGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCa                                                                                                                                            >  1:185003/1‑150 (MQ=255)
      gCAAGAAGGCCGTAGCCGAGCGCATCGTTTACGGTGCCCTGGATACCGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCa                                                                                                                                            >  1:322308/1‑150 (MQ=255)
      gCAAGAAGGCCGTAGCCGAGCGCATCGTTTACGGTGCCCTGGATACCGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCa                                                                                                                                            >  2:1784424/1‑150 (MQ=255)
       cAAGAAGGCCGTAGCCGAGCGCATCGTTTACGGTGCCCTGGATACCGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTctgcg                                                                                                                                                  <  2:2279665/143‑1 (MQ=255)
         agaagaCCGTAGCCGAGGGCATCGTTTACGGTGCCCTGGATACCGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACtt                                                                                                                                         >  2:157670/1‑150 (MQ=255)
          gaagGCCGTAGCCGAGCGCATCGTTTACGGTGCCCTGGATACCGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTa                                                                                                                                        <  2:322308/150‑1 (MQ=255)
          gaagGCCGTAGCCGAGCGCATCGTTTACGGTGCCCTGGATACCGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTa                                                                                                                                        >  1:2525368/1‑150 (MQ=255)
                cGTAGCCGAGCGCATCGTTTACGGTGCCCTGGATACAGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGt                                                                                                                                  >  1:1204790/1‑150 (MQ=255)
                     ccGAGCGCATCGTTTACGGTGCCCTGGATACCGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCgg                                                                                                                             <  2:3446266/150‑1 (MQ=255)
                       gAGCGCATCGTTTACGGTGCCCTGGATACCGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGtt                                                                                                                           <  1:2258264/150‑1 (MQ=255)
                          cgcATCGTTTACGGTGCCCTGGATACCGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGaa                                                                                                                        <  2:185003/150‑1 (MQ=255)
                           gcATCGTTTACGGTGCCCTGGATACCGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAg                                                                                                                       <  2:2525368/150‑1 (MQ=255)
                                gTTTACGGTGCCCTGGATACCGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGt                                                                                                                  <  1:376255/150‑1 (MQ=255)
                                 tttACGGTGCCCTGGATACCGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTccc                                                                                                                               <  1:3038245/136‑1 (MQ=255)
                                             tGGATACCGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑a                                                                                                    >  1:3212695/1‑150 (MQ=255)
                                              ggATACCGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑aa                                                                                                   >  2:2573827/1‑150 (MQ=255)
                                               gATACCGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AAc                                                                                                  <  1:764518/150‑1 (MQ=255)
                                                  acagTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGct                                                                                               <  2:1204790/147‑1 (MQ=255)
                                                   ccGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGaa                                                                                                                        <  1:2471153/125‑1 (MQ=255)
                                                    cGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGctct                                                                                             >  2:1284910/1‑150 (MQ=255)
                                                     gTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTg                                                                                            >  1:580848/1‑150 (MQ=255)
                                                     gTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTg                                                                                            <  1:374081/150‑1 (MQ=255)
                                                           gCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCTCTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATg                                                                                      >  2:3441778/1‑150 (MQ=255)
                                                             aCGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATgcg                                                                                    >  2:2887246/1‑150 (MQ=255)
                                                             aCGCAACAACAGCGACTCCCTGTAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATgcg                                                                                    >  2:3110730/1‑150 (MQ=255)
                                                               gCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATcc                                                                                                            >  1:670807/1‑125 (MQ=255)
                                                               gCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCt                                                                                  <  2:2287838/150‑1 (MQ=255)
                                                               gCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCt                                                                                  >  1:1993521/1‑150 (MQ=255)
                                                               gCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCGGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCt                                                                                  >  1:3565663/1‑150 (MQ=255)
                                                                cAAGAACAGGGACCCGCTGGAAATCTTGGAGAAAGCTCTCGAGGCCATCGCTCCGCTGGTGGAAGTAAAGTCCCGGCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTg                                                                                 <  2:1880186/150‑1 (MQ=255)
                                                                cAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTg                                                                                 <  2:1411818/150‑1 (MQ=255)
                                                                 aaGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCg                                                                                                                              >  2:2231439/1‑105 (MQ=255)
                                                                 aaGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCg                                                                                                                              <  1:2231439/105‑1 (MQ=255)
                                                                 aaGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATc                                                                                                             <  2:3821745/122‑1 (MQ=255)
                                                                 aaGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATc                                                                                                             >  1:3821745/1‑122 (MQ=255)
                                                                 aaGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTgg                                                                                >  1:51136/1‑150 (MQ=255)
                                                                   gAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCTCGGTTGAAGTTc                                                                                                                    >  2:1673954/1‑113 (MQ=255)
                                                                   gAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCt                                                                              >  1:2506955/1‑150 (MQ=255)
                                                                    aaCAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTc                                                                             >  1:2072967/1‑150 (MQ=255)
                                                                       aGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTc                                                                                                                    <  2:552568/109‑1 (MQ=255)
                                                                       aGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTc                                                                                                                    >  1:552568/1‑109 (MQ=255)
                                                                       aGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTa                                                                          <  1:2314898/150‑1 (MQ=255)
                                                                       aGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTa                                                                          <  2:3207416/150‑1 (MQ=255)
                                                                       aGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTa                                                                          <  1:1194472/150‑1 (MQ=255)
                                                                       aGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTa                                                                          >  2:3670065/1‑150 (MQ=255)
                                                                        gCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAg                                                                         >  2:18583/1‑150 (MQ=255)
                                                                        gCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAg                                                                         <  2:51136/150‑1 (MQ=255)
                                                                           aCCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACt                                                                      <  1:2667788/150‑1 (MQ=255)
                                                                             ccccTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTAc                                                                    >  2:2641576/1‑150 (MQ=255)
                                                                             ccccTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTAc                                                                    <  1:1377974/150‑1 (MQ=255)
                                                                              cccTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTa                                                                          <  1:1315215/143‑1 (MQ=255)
                                                                              cccTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTa                                                                          >  2:1315215/1‑143 (MQ=255)
                                                                                cTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAg                                                                                                                                    >  2:3275837/1‑84 (MQ=255)
                                                                                cTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGcc                                                                 >  2:649163/1‑150 (MQ=255)
                                                                                 tGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGc                                                                                                <  2:3727093/118‑1 (MQ=255)
                                                                                 tGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGc                                                                                                >  1:3727093/1‑118 (MQ=255)
                                                                                  ggAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCg                                                               >  2:3107194/1‑150 (MQ=255)
                                                                                        cTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAgcg                                                         >  1:708773/1‑150 (MQ=255)
                                                                                         ttCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCcgtcgt                                                                                                      <  1:909042/105‑1 (MQ=255)
                                                                                         ttCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTgg                                                                                           <  1:1957629/115‑1 (MQ=255)
                                                                                          tCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAGTGCGCTGGCTCGTAGACTACGCCCGCAAGcgcg                                                       <  2:3755500/150‑1 (MQ=255)
                                                                                            gagaAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTc                                                                                                                 >  2:3838454/1‑91 (MQ=255)
                                                                                            gagaAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTc                                                                                                                 <  1:3838454/91‑1 (MQ=255)
                                                                                            gagaAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCt                                                                                  <  1:1019418/121‑1 (MQ=255)
                                                                                            gagaAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCt                                                                                  >  2:1019418/1‑121 (MQ=255)
                                                                                            gagaAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCgcggc                                                     <  1:3670065/150‑1 (MQ=255)
                                                                                                 aGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCGAGaa                                                >  1:1699462/1‑150 (MQ=255)
                                                                                                    tctcGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCgcggc                                                     >  1:1049386/1‑142 (MQ=255)
                                                                                                    tctcGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCgcggc                                                     <  2:1049386/142‑1 (MQ=255)
                                                                                                      tcGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGt                                                                                                                  <  2:381963/80‑1 (MQ=255)
                                                                                                      tcGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGt                                                                                                                  >  1:381963/1‑80 (MQ=255)
                                                                                                      tcGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGctct                                                                                             >  2:766145/1‑100 (MQ=255)
                                                                                                      tcGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGctct                                                                                             <  1:766145/100‑1 (MQ=255)
                                                                                                      tcGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCGAGAAGTCGa                                           <  1:1784424/150‑1 (MQ=255)
                                                                                                      tcGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAACGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCGAGAAGTCGa                                           >  1:284965/1‑150 (MQ=255)
                                                                                                      tcGACGCCATCGATCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAAt                                                                                       <  2:2442812/106‑1 (MQ=255)
                                                                                                      tcGACGCCATCGATCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAAt                                                                                       >  1:2442812/1‑106 (MQ=255)
                                                                                                      tcGACGCCATCACTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCATAGACTACGCCCGCAAGCGCGGCGAGAAGTCGa                                           <  1:2293665/150‑1 (MQ=255)
                                                                                                       cGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCGAGAAGTCGAt                                          <  2:1974696/150‑1 (MQ=255)
                                                                                                             cATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCGAGAAGTCGATGGctct                                    >  1:3218733/1‑150 (MQ=255)
                                                                                                              aTCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCGAGAAGTCGATGGCTCTg                                   >  2:2235813/1‑150 (MQ=255)
                                                                                                               ccGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCGAGAAGTCGATGGCTCTGc                                  <  1:3404813/149‑1 (MQ=255)
                                                                                                                   tCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCga                                                   >  2:2895354/1‑129 (MQ=255)
                                                                                                                   tCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCga                                                   <  1:2895354/129‑1 (MQ=255)
                                                                                                                           tCGAAGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCGAGAAGTCGATGGCTCTGCGTCTGGCCGGCg                      >  2:1169588/1‑150 (MQ=255)
                                                                                                                              aaGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTGCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCGAGAAGTCGATGGCTCTGCGTCTGGCCGGCGAAc                   >  2:2013034/1‑150 (MQ=255)
                                                                                                                               aGTAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCAGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCGAGAAGTCGATGGCTCTGCGTCTGGCCGGCGAAct                  <  1:240480/150‑1 (MQ=255)
                                                                                                                                 tAAAGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCGAGAAGTCGATGGCTCTGCGTCTGGCCGGCGAActgc                >  2:2084225/1‑150 (MQ=255)
                                                                                                                                  aaaGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGTCCACGCTC‑GGTAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCGAGAAGTCGATGGCTCTGCGTCTGGCCGGCGAActgct               <  1:3110730/150‑1 (MQ=255)
                                                                                                                                   aaGTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCGAGAAGTCGATGGCTCTGCGTCTGGCCGGCGAActgctg              >  2:2374980/1‑150 (MQ=255)
                                                                                                                                   aaGTCCCGTCGTGTCTGCGGTGCCACTTACAAGGACCCGGTTGAAGGTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCGAGAAGTCGATGGCTCTGCGTCTGGCCGGCGAActgctg              >  2:3313148/1‑150 (MQ=255)
                                                                                                                                   aaGTCCCGTAGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCGAGAAGTCGATGGCTCTGCGTCTGGCCGGCGAActgctg              <  1:1641844/150‑1 (MQ=255)
                                                                                                                                     gTCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCGAGAAGTCGATGGCTCTGCGTCTGGCCGGCGAACTGCTGGa            >  2:908802/1‑150 (MQ=255)
                                                                                                                                      tCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAgcgc                                                        <  1:1797053/105‑1 (MQ=255)
                                                                                                                                      tCCCGTCGTGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCGAGAAGTCGATGGCTCTGCGTCTGGCCGGCGAACTGCTGGAt           >  2:2353206/1‑150 (MQ=255)
                                                                                                                                         cgtcgtGTCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCGAGAAGTCGATGGCTCTGCGTCTGGCCGGCGAACTGCTGGAtgct        >  2:676965/1‑150 (MQ=255)
                                                                                                                                              tgtCTGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCGAGAAGTCGATGGCTCTGCGTCTGGCCGGCGAACTGCTGGATGCTGCCGa   <  2:1993521/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AAAGCGGCAAGAAGGCCGTAGCCGAGCGCATCGTTTACGGTGCCCTGGATACCGTCAAAGCACGCAAGAACAGCGACCCCCTGGAAATCTTCGAGAAAGCTCTCGACGCCATCGCTCCGCTGGTCGAAGTAAAGTCCCGTCGTGTCGGCGGTGCCACTTACCAGGTCCCGGTTGAAGTTCGTCCATCCCGTCGT‑AACGCTCTGGCAATGCGCTGGCTCGTAGACTACGCCCGCAAGCGCGGCGAGAAGTCGATGGCTCTGCGTCTGGCCGGCGAACTGCTGGATGCTGCCGAA  >  NC_002947/546747‑547039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑