| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,648,882 | +T | intergenic (‑119/+177) | oprB‑II ← / ← PP_1446 | carbohydrate‑selective porin/TonB‑dependent receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,648,881 | 1 | . | T | 90.0% | 146.3 / 10.5 | 50 | intergenic (‑118/+178) | oprB‑II/PP_1446 | carbohydrate‑selective porin/TonB‑dependent receptor |
| Reads supporting (aligned to +/- strand): ref base . (3/2); new base T (21/24); total (24/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
TGTAGCAGGTTTTAGGCAGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGA‑TATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAA > NC_002947/1648736‑1649025 | tGTAGCAGGTTTTAGGCAGTTGTAACATGAGATAGCGCTATCTTTGGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGa‑ttat < 2:1089784/150‑4 (MQ=255) aGCAGGTTTTAGGCAGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAgg < 1:1000138/150‑1 (MQ=255) aGCAGGTTTTAGGCAGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAgg < 2:1237043/150‑1 (MQ=255) gCAGGTTTTAGGCAGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCATTTCAGCTTGCGGCGGGGA‑GGATTATAggg < 1:1181565/150‑1 (MQ=255) gTTTTAGGCAGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGAGCCAAGATCAATCGATGCACCGCTGGAGTGCGCGGCGGTGAGGGT‑TAGAGGGGAAg > 2:455912/1‑150 (MQ=255) tttAGGCAGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGtt < 2:196529/150‑1 (MQ=255) cATGAGATAGCGCGATCGTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACa > 2:969027/1‑150 (MQ=255) gagaTAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAAt > 1:804245/1‑150 (MQ=255) gagaTAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAAt > 2:1198397/1‑150 (MQ=255) tAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTc < 1:455912/150‑1 (MQ=255) cTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCaga < 1:411266/150‑1 (MQ=255) tATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCagag > 1:684240/1‑150 (MQ=255) ttttGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGa‑ttata > 2:1199847/1‑105 (MQ=255) ttttGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGa‑ttata < 1:1199847/109‑5 (MQ=255) ttttGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGA‑TATAGGGGAAg > 1:391576/1‑115 (MQ=255) ttttGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGA‑TATAGGGGAAg < 2:391576/115‑1 (MQ=255) tttGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGAttg > 2:123637/1‑150 (MQ=255) gATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGAttgttg < 1:1159717/150‑1 (MQ=255) attattGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGAttgttgt < 2:338237/150‑1 (MQ=255) ttGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGaa > 1:877531/1‑150 (MQ=255) tGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAAc < 2:804245/150‑1 (MQ=255) gAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGAttgttgt > 1:92741/1‑144 (MQ=255) gAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGAttgttgt < 2:92741/144‑1 (MQ=255) aaaaaaaCGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACg > 2:957071/1‑149 (MQ=255) aaaaaaaCGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACg < 1:957071/149‑1 (MQ=255) aCGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACa > 1:204079/1‑150 (MQ=255) aCGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACa > 2:593724/1‑150 (MQ=255) cAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAgtcg > 1:1073035/1‑150 (MQ=255) aGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAg < 2:204079/150‑1 (MQ=255) ggcgggcCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATc < 2:1228256/145‑1 (MQ=255) ggcgggcCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATc > 1:1228256/1‑145 (MQ=255) ggcCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGgt < 1:1198397/150‑1 (MQ=255) cTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACa < 1:685397/123‑1 (MQ=255) cTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACa > 2:685397/1‑123 (MQ=255) tCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGa > 1:1138865/1‑150 (MQ=255) tCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTCGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGa < 1:362089/150‑1 (MQ=255) aaTGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGc < 1:199598/150‑1 (MQ=255) aTGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGA‑TATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGAtt < 1:548379/100‑1 (MQ=255) aTGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGA‑TATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGAtt > 2:548379/1‑100 (MQ=255) ggATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCggg < 2:1138865/150‑1 (MQ=255) gCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCAccc > 1:1202213/1‑150 (MQ=255) gACAAGATTGTTCGAGGATGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGNCAACAGTCGTCATTAACCGAGGCAGGTGTAGGAGCGGGTTCACCcgc < 1:198330/150‑1 (MQ=255) cAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTa > 1:1263259/1‑131 (MQ=255) cAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTa < 2:1263259/131‑1 (MQ=255) aTTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGAttgttgt < 2:810980/89‑1 (MQ=255) aTTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGAttgttgt > 1:810980/1‑89 (MQ=255) ttCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCg < 1:123637/150‑1 (MQ=255) ttCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCg > 2:604427/1‑150 (MQ=255) gATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGtt > 2:1064814/1‑150 (MQ=255) tCAGCTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATc < 2:877531/150‑1 (MQ=255) cTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCcgc > 2:952475/1‑122 (MQ=255) cTTGCGGCGGGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCcgc < 1:952475/122‑1 (MQ=255) ggcggGGA‑GGATTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTcgcg > 2:510577/1‑150 (MQ=255) ga‑ggatTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGaa > 2:824468/1‑114 (MQ=255) ga‑ggatTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGaa < 1:824468/114‑1 (MQ=255) ga‑ggatTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGaa > 2:311175/1‑150 (MQ=255) | TGTAGCAGGTTTTAGGCAGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGA‑GGA‑TATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAA > NC_002947/1648736‑1649025 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |