| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,633,616 | G→T | 36.0% | W12L (TGG→TTG) | PP_2304 → | peptidyl‑prolyl cis‑trans isomerase D |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,633,616 | 0 | G | T | 36.0% | 6.6 / 7.2 | 14 | W12L (TGG→TTG) | PP_2304 | peptidyl‑prolyl cis‑trans isomerase D |
| Reads supporting (aligned to +/- strand): ref base G (4/5); new base T (2/3); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01 | |||||||||||
ACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGCCTTATCGTCGTGTTGATGGCATTGACCGGTTTCGAAGCCATTTTCCAGGCCGCCACTCATAGCCAGGACGCCGCCAAGGTGAACGGC > NC_002947/2633473‑2633725 | acacGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGc < 1:338113/150‑1 (MQ=255) tttGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGa < 1:294495/150‑1 (MQ=255) ggaaaGACGCATCGCCGGCTGCGCCTTTCTTTTATAAGCATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTGGGATTGCCAAGCCcctcagc > 2:64442/3‑148 (MQ=255) aaaGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCgg > 2:300165/1‑150 (MQ=255) tCGGATGCGCCTTTCTTTTATCAGACTTGTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGCCGCATGCTGCAAAATCTCAGTTTCTATACGCCAGTTTTGATTGCCAAGACCATCATCGGCCTTATcgtcgt < 1:300165/150‑1 (MQ=255) aTGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGCCTTATCGTCGTGTTg > 2:51504/1‑150 (MQ=255) ccACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGCCTTATCGTCGTGTTGATGGCATTGACCGGTTTCAAAGCCAttt > 2:7995/1‑150 (MQ=255) cattGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCTGCCTTATCGTCGTGTTGATGGCATTGACCGGTTTCGAAGCCATTTTCCAGGCCGCCACTCATAGCCa < 2:120633/147‑1 (MQ=255) ttCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGCCTTATCGTCGTGTTGATGGCATTGACCGGTTTCGAAGCCATTTTCCAGGCCGCCACTCATAGCCAGGACGCCGCCAAGGt < 2:200941/150‑1 (MQ=255) ggACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGCCTTATCGTCGTGTTGATGGCATTGACCGGTTTCGAAGCCATTTTCCAGGCCGCCACTCATAGCCAGGACGCCGCCAAGGTGAACGGc < 1:51504/150‑1 (MQ=255) cacaAGGTTTGATTGCCAAGACCATCATCGGCCTTATCGTCGTGTTGATGGCATTGACCGGTTTCGAAGCCATTTTCCAGGCCGCCACTCATAGCCAGGa > 1:335019/1‑100 (MQ=255) cacaAGGTTTGATTGCCAAGACCATCATCGGCCTTATCGTCGTGTTGATGGCATTGACCGGTTTCGAAGCCATTTTCCAGGCCGCCACTCATAGCCAGGa > 1:335173/1‑100 (MQ=255) cacaAGGTTTGATTGCCAAGACCATCATCGGCCTTATCGTCGTGTTGATGGCATTGACCGGTTTCGAAGCCATTTTCCAGGCCGCCACTCATAGCCAGGa < 2:335019/100‑1 (MQ=255) cacaAGGTTTGATTGCCAAGACCATCATCGGCCTTATCGTCGTGTTGATGGCATTGACCGGTTTCGAAGCCATTTTCCAGGCCGCCACTCATAGCCAGGa < 2:335173/100‑1 (MQ=255) | ACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGCCTTATCGTCGTGTTGATGGCATTGACCGGTTTCGAAGCCATTTTCCAGGCCGCCACTCATAGCCAGGACGCCGCCAAGGTGAACGGC > NC_002947/2633473‑2633725 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |