| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,935,428 | G→A | 37.0% | V34I (GTC→ATC) | PP_2568 → | ring‑cleaving dioxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,935,428 | 0 | G | A | 37.0% | 0.8 / 5.7 | 11 | V34I (GTC→ATC) | PP_2568 | ring‑cleaving dioxygenase |
| Reads supporting (aligned to +/- strand): ref base G (3/3); new base A (2/2); total (5/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01 | |||||||||||
TATGGTGGCGAACGTATTCACAAACACGCCACAGGGAGCCCGGCATGCGGCCTTTCACCATCAAGCACATCGACCACCTGGTGTTGCGGGTCAGCGACCTGCCGCGCAGTGTTGCCTTTTACACCGAACTGCTTGGCTGCACCGTCAGCCGCGTGCGCGAAGACCTGGGCATGGTGCACCTGGCCACGGGCACGGCGATGATCGACCTGGTAACCCTGGATGGCCCGCTGGGGCAGCCAGGCGGAGCTGCACCGGGCGTGGAAGGGCGCAACCTGCACCATTTCTGC > NC_002947/2935285‑2935571 | tATGGTGGCGAACGTATTCACAAACACGCCACAGGGAGCCCGGCATGCGGCCTTTCACCATCAAGCACATCGACCACCTGGTGTTGCGGGTCAGCGACCTGCCGCGCAGTGTTGCCTTTTACACCGAACTACTTGGTTTCACCGTCAGcc < 2:131642/150‑1 (MQ=255) acaAACACGCCACAGGGAGCCCGGCATGCGGCCTTTCACCATCAAGCACATCGACCACCTGGTGTTGCGGGTCAGCGACCTGCCGCGCAGTGTTGCCTTTTACACCGAACTGCTTGGCTGCACCATCAGcc > 1:243160/1‑131 (MQ=255) acaAACACGCCACAGGGAGCCCGGCATGCGGCCTTTCACCATCAAGCACATCGACCACCTGGTGTTGCGGGTCAGCGACCTGCCGCGCAGTGTTGCCTTTTACACCGAACTGCTTGGCTGCACCATCAGcc > 1:243197/1‑131 (MQ=255) acaAACACGCCACAGGGAGCCCGGCATGCGGCCTTTCACCATCAAGCACATCGACCACCTGGTGTTGCGGGTCAGCGACCTGCCGCGCAGTGTTGCCTTTTACACCGAACTGCTTGGCTGCACCATCAGcc < 2:243160/131‑1 (MQ=255) acaAACACGCCACAGGGAGCCCGGCATGCGGCCTTTCACCATCAAGCACATCGACCACCTGGTGTTGCGGGTCAGCGACCTGCCGCGCAGTGTTGCCTTTTACACCGAACTGCTTGGCTGCACCATCAGcc < 2:243197/131‑1 (MQ=255) cACCATCAAGCACATCGACCACCTGGTGTTGCGGGTCAGCGACCTGCCGCGCAGTGTTGCCTTTTACACCGAACTGCTTGGCTGCACCGTCAGCCGCGTGCGCGAAGACCTGGGCATGGTGCACCTGGCCACGGGCACGGCGATGATCGa > 1:367740/1‑150 (MQ=255) aGCGACCTGCCGCGCAGTGTTGCCTTTTACACCGAACTGCTTGGCTGCACCGTCAGCCGCGTGCGCGAAGACCTGGGCATGGTGCACCTGGCCACGGGCACGGCGATGATCGACCTGGTAACCCTGGATGGCCCGCTGGGGCAGCCAGGc < 2:69839/150‑1 (MQ=255) aGTGTTGCCTTTTACACCGAACTGCTTGGCTGCACCGTCAGCCGCGTGCGCGAAGACCTGGGCATGGTGCACCTGGCCACGGGCACGGCGATGATCGACCTGGTAACCCTGGATGGCCCGCTGGGGCAGCCAGGCGGAGCTGCACCGGGc > 2:105094/1‑150 (MQ=255) cTTTTACACCGAACTGCTTGGCTGCACCTTCAGCCGCGTGCGCGAAGACCTGGGCATGGTGCACCTGGCCACGGGCACGGCGATGATCGACCTGGTAACCCTGGATGGCCCGCTGGGGCAGCCAGGCGGAGCTGCACCGGGCGTGGAAgg < 2:367740/150‑1 (MQ=255) tGCTTGGCTGCACCGTCAGCCGCGTGCGCGAAGACCTGGGCATGGTGCACCTGGCCACGGGCACGGCGATGATCGACCTGGTAACCCTGGATGGCCCGCTGGGGCAGCCAGGCGGAGCTGCACCGGGCGTGGAAGGGCGCAACCTGCAcc > 1:392462/1‑150 (MQ=255) tGCACCGTCAGCCGCGTGCGCGAAGACCTGGGCATGGTGCACCTGGCCACGGGCACGGCGATGATCGACCTGGTAACCCTGGATGGCCCGCTGGGGCAGCCAGGCGGAGCTGCACCGGGCGTGGAAGGGCGCAACCTGCACCATTTctgc < 1:105094/150‑1 (MQ=255) | TATGGTGGCGAACGTATTCACAAACACGCCACAGGGAGCCCGGCATGCGGCCTTTCACCATCAAGCACATCGACCACCTGGTGTTGCGGGTCAGCGACCTGCCGCGCAGTGTTGCCTTTTACACCGAACTGCTTGGCTGCACCGTCAGCCGCGTGCGCGAAGACCTGGGCATGGTGCACCTGGCCACGGGCACGGCGATGATCGACCTGGTAACCCTGGATGGCCCGCTGGGGCAGCCAGGCGGAGCTGCACCGGGCGTGGAAGGGCGCAACCTGCACCATTTCTGC > NC_002947/2935285‑2935571 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |