| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,080,327 | A→T | 19.1% | intergenic (‑144/+24) | csrA ← / ← lysC | carbon storage regulator/aspartokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,080,327 | 0 | A | T | 19.1% | 41.4 / 6.8 | 21 | intergenic (‑144/+24) | csrA/lysC | carbon storage regulator/aspartokinase |
| Reads supporting (aligned to +/- strand): ref base A (9/8); new base T (2/2); total (11/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.34e-01 | |||||||||||
AAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCGGAAGTGGAGATCATCT > NC_002947/5080201‑5080472 | aaaCATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCAAAGGGAGCGGCAAGCCGCGCCCTTCCAGGCAGCGcc > 2:148141/1‑150 (MQ=255) aCATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCtt > 2:192304/1‑150 (MQ=255) ggTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCt > 2:391658/1‑150 (MQ=255) tAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCGAGCCGCGCCGTTCCATGCAGCGCCTTACTCGCCCTGt > 2:246300/1‑150 (MQ=255) cAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCgg > 1:284312/1‑150 (MQ=255) tCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCa < 1:5232/150‑1 (MQ=255) gctgggATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCa < 1:377756/146‑1 (MQ=255) ggCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGa > 1:58454/1‑150 (MQ=255) gTTACTCGCCGCACAAGGCAGGACAGGACCTGCGAGGATACGAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAg < 1:246300/150‑1 (MQ=255) tCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCa > 2:195440/1‑150 (MQ=255) gcACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTAc < 1:192304/150‑1 (MQ=255) aaGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCTTGCCGCGCCCTTc > 1:341465/1‑62 (MQ=37) aaGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCTTGCCGCGCCCTTc < 2:341465/62‑1 (MQ=37) ggacaggacCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGa > 1:264798/1‑150 (MQ=255) acCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGcaccac < 1:195440/150‑1 (MQ=255) ggcgAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCACTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCt < 1:148141/150‑1 (MQ=255) tggAAGGGCGCGGCTTGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGAt < 2:159741/139‑1 (MQ=255) tggAAGGGCGCGGCTTGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGAt > 1:159741/3‑141 (MQ=255) ggCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCGGAAGTGGAGat > 1:376164/1‑150 (MQ=255) ggCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCGGAAGTGGAGATCATCt < 2:284312/150‑1 (MQ=255) ggCAAGCCGCGCCCTTCAAGGCAGCGCCTTACTCGCCCTGTCGGCCAGGGGCATCTAGATCGAACGCGGTATGACCCGAACGTACGGCCTGCCCCTGGCAATTGGCCTCGTGGACCGCCGAAACCTTGATCTCGGAAGTGGAGATCATCt < 1:121348/150‑1 (MQ=255) | AAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCGGAAGTGGAGATCATCT > NC_002947/5080201‑5080472 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |