Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,567,230 A→T 12.9% L103M (TTG→ATG) ‡ mutL ← DNA mismatch repair protein MutL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,567,2300AT12.9% 82.8 / 4.3 31L103M (TTG→ATG) ‡mutLDNA mismatch repair protein MutL
Reads supporting (aligned to +/- strand):  ref base A (15/12);  new base T (2/2);  total (17/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

TTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGCCATCATCCCGCACCTTCAGCAGTTTGAC  >  NC_002947/5567087‑5567374
                                                                                                                                               |                                                                                                                                                
ttCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTc                                                                                                                                            <  1:280885/150‑1 (MQ=255)
                              ctgCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTc                                                                                                              <  1:377632/148‑1 (MQ=255)
                                       ccGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGGGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTc                                                                                                     <  1:332154/150‑1 (MQ=255)
                                       ccGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTc                                                                                                     >  1:355255/1‑150 (MQ=255)
                                         tcggcgTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCa                                                                                                   <  2:49430/149‑1 (MQ=255)
                                         gcggcgTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGACACAATACCCCCTCTCGGGCTTcaa                                                                                                   >  2:248319/1‑148 (MQ=255)
                                            gcgTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCt                                                                                                >  1:378579/1‑150 (MQ=255)
                                               tCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCTTGGCCTCgatc                                                                                                                                        >  2:300742/1‑104 (MQ=255)
                                               tCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCTTGGCCTCgatc                                                                                                                                        <  1:300742/107‑4 (MQ=255)
                                                          ccTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGTTACGCGATGTCAGGGTCAGTCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGTCTCAATACCCCTTCTATGTCTTCCAGCGCGCGGATCTTGCtt                                                                                  <  2:313886/150‑2 (MQ=255)
                                                            ttCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATAc                                                                                                                    >  2:139015/1‑114 (MQ=255)
                                                            ttCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATAc                                                                                                                    <  1:139015/114‑1 (MQ=255)
                                                                     tACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCg                                                                       >  2:136701/1‑150 (MQ=255)
                                                                     tACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCg                                                                       >  1:345332/1‑150 (MQ=255)
                                                                              ggCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCtg                                                                                  <  2:214471/130‑1 (MQ=255)
                                                                              ggCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCtg                                                                                  >  1:214471/1‑130 (MQ=255)
                                                                                          actggcGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAg                                                                 >  1:413295/1‑135 (MQ=255)
                                                                                          actggcGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAg                                                                 <  2:413295/135‑1 (MQ=255)
                                                                                               cGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCgg                                             <  2:345332/150‑1 (MQ=255)
                                                                                                     gcgAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGaaa                                       >  1:243432/1‑150 (MQ=255)
                                                                                                          gtcagggtcaggCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCg                                  >  1:268462/1‑150 (MQ=255)
                                                                                                           tcagggtcaggCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGc                                 >  2:443483/1‑150 (MQ=255)
                                                                                                            cagggtcaggCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCt                                <  1:17937/150‑1 (MQ=255)
                                                                                                               ggtcaggCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGcc                             >  2:143757/1‑150 (MQ=255)
                                                                                                                         gcCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTTTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGCCATCATCCCGc                   >  2:273276/1‑150 (MQ=255)
                                                                                                                         gcCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCTGCAGAAATGCCGCTGCCATCATCCCGc                   >  2:208988/1‑150 (MQ=255)
                                                                                                                               gAACTGCACGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGCCATCATCCCGCACCTTc             >  2:250723/1‑150 (MQ=255)
                                                                                                                                aaCTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGCCATCATCCCGCACCTTca            <  2:268462/150‑1 (MQ=255)
                                                                                                                                    tggcGAGGCCTTGGCCTCGCCACGTAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAg                                                     >  2:250589/4‑105 (MQ=255)
                                                                                                                                    tggcGAGGCCTTGGCCTCGCCACGTAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAg                                                     <  1:250589/102‑1 (MQ=255)
                                                                                                                                          ggCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGCCATCATCCCGCACCTTCAGCAGTTTGAc  <  2:378579/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
TTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGCCATCATCCCGCACCTTCAGCAGTTTGAC  >  NC_002947/5567087‑5567374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑