Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,900,014 C→G 16.0% Q310H (CAG→CAC)  PP_5173 ← RND family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,900,0140CG16.0% 57.1 / 6.4 25Q310H (CAG→CAC) PP_5173RND family transporter
Reads supporting (aligned to +/- strand):  ref base C (11/10);  new base G (2/2);  total (13/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.74e-01

CAAGACTGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCGATGTCGGCGATATC  >  NC_002947/5899872‑5900163
                                                                                                                                              |                                                                                                                                                     
cAAGACTGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCa                                                                                                                                                >  2:367137/1‑150 (MQ=255)
  aGACTGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACCCCCACCGGCAGGTCCTGGACCACCTGGTCCATg                                                                                                                                              <  2:391438/150‑1 (MQ=255)
   gACTGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATgc                                                                                                                                             >  2:336772/1‑150 (MQ=255)
   gACTGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCATGGACCAGGTGGTCCagga                                                                                                                                             <  2:374584/150‑4 (MQ=255)
      tGACAACGCTCACGACCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCAt                                                                                                                                               <  2:363037/145‑1 (MQ=255)
      tGACAAAGCTCACGACCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGGCCTGGACCACCTGGTCCAt                                                                                                                                               >  1:363037/1‑145 (MQ=255)
          aaaGCTCAGGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACAACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAACTGGGCCATGCGCttttt                                                                                                                                      >  1:108448/1‑150 (MQ=255)
                                 ccaccGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGa                                                                                                               >  2:71820/1‑150 (MQ=255)
                                        cTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTc                                                                                                                                     >  1:69410/1‑121 (MQ=255)
                                        cTCGAACAGGGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTc                                                                                                                                     <  2:69410/121‑1 (MQ=255)
                                                      tGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGc                                                                                          <  2:379726/150‑1 (MQ=255)
                                                        gTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCATGGACCAGGTGGTCCagggc                                                                                                                                            >  1:444078/1‑94 (MQ=255)
                                                        gTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCATGGACCAGGTGGTCCagggc                                                                                                                                            <  2:444078/98‑5 (MQ=255)
                                                                       gcctGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATg                                                                         <  1:367137/150‑1 (MQ=255)
                                                                       gcctGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATg                                                                         >  1:282404/1‑150 (MQ=255)
                                                                       gcctGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATg                                                                         <  2:282404/150‑1 (MQ=255)
                                                                        cctGCTCGTCCTGCACGTCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAACTGGTCCATGCGCTTTTTTCACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGTCGAGGCCAATgg                                                                        <  2:108448/150‑1 (MQ=255)
                                                                             ttGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTc                                                                   >  1:368024/1‑150 (MQ=255)
                                                                                            tGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACgg                                                    >  1:12001/1‑150 (MQ=255)
                                                                                             ggTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGa                                                   <  1:336772/150‑1 (MQ=255)
                                                                                              gTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCAACCATCATCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGaa                                                  >  1:250777/1‑150 (MQ=255)
                                                                                                     aCGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGc                                           <  2:12001/150‑1 (MQ=255)
                                                                                                                   gACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGAc                             <  1:169261/150‑1 (MQ=255)
                                                                                                                                   gcatGGACCAGGTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCGATg             >  1:401847/4‑150 (MQ=255)
                                                                                                                                     cTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCGATGTc           >  1:91393/1‑150 (MQ=255)
                                                                                                                                           caccTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCGGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCGATGTCGGCGat     >  2:5424/1‑150 (MQ=255)
                                                                                                                                              cTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCGATGTCGGCGATATc  >  2:388657/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                     
CAAGACTGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACCTGGTCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCGATGTCGGCGATATC  >  NC_002947/5899872‑5900163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑