| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,960,051 | A→T | 16.7% | noncoding (74/103 nt) | PP_mr63 ← | rnk_pseudo ncRNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,960,051 | 0 | A | T | 16.7% | 56.0 / 5.2 | 24 | noncoding (74/103 nt) | PP_mr63 | rnk_pseudo ncRNA |
| Reads supporting (aligned to +/- strand): ref base A (10/10); new base T (2/2); total (12/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
GTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCG > NC_002947/5959906‑5960188 | gTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAg > 2:82713/1‑148 (MQ=255)gTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAg < 1:82713/148‑1 (MQ=255) cgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGC‑AAACCCCGCCCTTGGGCGGGGttttga < 2:119511/150‑4 (MQ=255) ggCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGaa < 1:241324/150‑1 (MQ=255) tCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCtt > 1:58110/1‑150 (MQ=255) gATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTg < 2:313810/150‑1 (MQ=255) tCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGc > 2:428492/1‑150 (MQ=255) aCGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCa > 2:195751/1‑150 (MQ=255) gCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCTTGGGCGGGGtttt < 1:311738/122‑2 (MQ=255) gCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCTTGGGCGGGGtttt > 2:311738/1‑121 (MQ=255) gATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGc > 2:150166/1‑150 (MQ=255) gggAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATa > 2:352742/1‑150 (MQ=255) ggCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCaa < 1:352742/150‑1 (MQ=255) gCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTgc < 1:195751/150‑1 (MQ=255) aTCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAgt < 1:150166/150‑1 (MQ=255) aGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAACCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCNACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGc < 1:158204/150‑1 (MQ=255) gTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCTACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGcc > 2:415426/1‑150 (MQ=255) aGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCTACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCaa < 1:415426/150‑1 (MQ=255) tCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGc < 2:58110/150‑1 (MQ=255) tgCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCt < 2:274383/119‑1 (MQ=255) tgCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCt > 1:274383/1‑119 (MQ=255) cAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGc > 1:89354/1‑150 (MQ=255) gCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGc > 1:320372/1‑150 (MQ=255) caaaCCCCGCCCTTGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCg > 1:107645/2‑150 (MQ=255) | GTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCG > NC_002947/5959906‑5960188 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |