| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 210,573 | A→T | 19.1% | E5360V (GAG→GTG) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 210,573 | 0 | A | T | 19.1% | 41.5 / 4.5 | 22 | E5360V (GAG→GTG) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base A (4/12); new base T (2/2); total (6/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAA > NC_002947/210424‑210720 | cACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACcgacga < 1:200335/150‑1 (MQ=255) ccGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCTCGTCGGTGACCGACGTGctgaccg < 2:366438/139‑7 (MQ=14) ccGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCTCGTCGGTGACCGACGTGctgaccg > 1:366438/1‑133 (MQ=11) gACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGaa < 2:345496/150‑1 (MQ=255) gACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGaa < 1:406237/150‑1 (MQ=255) cGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGa < 1:421270/150‑1 (MQ=255) tACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGCGCCAGGTACCCCAGGCAACGAAGGCGACCTg < 2:274808/150‑1 (MQ=255) ggCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAg < 2:243598/150‑1 (MQ=255) aaCGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCAcc > 1:388103/1‑150 (MQ=255) gACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATc < 1:440272/150‑1 (MQ=255) ccTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCAcc < 1:176317/150‑1 (MQ=255) cGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGAc > 2:254869/1‑133 (MQ=255) cGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGAc < 1:254869/133‑1 (MQ=255) ttGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGTGc < 2:445910/78‑1 (MQ=255) ggCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAAc < 1:223340/150‑1 (MQ=255) cGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGAc < 2:388103/150‑1 (MQ=255) aCAAGACGCCGGTCAGCACGTCGGCCACCGACGCGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTgg < 2:157980/150‑1 (MQ=255) aGACGCCGGTCAGCCCGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTc < 1:96936/150‑1 (MQ=37) tggctcGTCGGTGACCGACGTGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGt > 1:424311/6‑150 (MQ=255) gTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGAc > 1:385787/1‑150 (MQ=35) tCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGa > 1:275110/1‑150 (MQ=35) tCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGa < 2:425367/150‑1 (MQ=35) tCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGG‑TCACTACCAGGTCGTGagccagcg < 2:262848/150‑8 (MQ=11) cgaGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGcaacaa < 1:171343/150‑1 (MQ=35) | CACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAA > NC_002947/210424‑210720 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |