| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,023,216 | T→C | 19.1% | intergenic (+75/+69) | PP_5273 → / ← PP_5274 | oxidoreductase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,023,216 | 0 | T | C | 19.1% | 35.3 / 4.4 | 21 | intergenic (+75/+69) | PP_5273/PP_5274 | oxidoreductase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (8/9); new base C (2/2); total (10/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01 | |||||||||||
AAGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCG > NC_002947/6023076‑6023363 | aaGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCACGCCCGCtac < 2:64723/150‑3 (MQ=255) gcgcTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGt < 2:323102/150‑1 (MQ=255) ggCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAgg > 1:185513/1‑150 (MQ=255) tCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGc < 1:393214/150‑1 (MQ=255) tGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgt < 2:185513/150‑1 (MQ=255) aCTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgtgt < 1:342040/150‑1 (MQ=255) ttGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCa < 2:341287/150‑1 (MQ=255) cccATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCa > 1:313485/1‑150 (MQ=255) aTCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGCCCATCGTGTCAGTCCTTCCAGaa > 2:98574/1‑150 (MQ=255) cTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTggg < 1:126357/103‑1 (MQ=255) cTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTggg > 2:126357/1‑103 (MQ=255) gcagcaACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTgg < 1:182723/96‑1 (MQ=255) gcagcaACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTgg > 2:182723/1‑96 (MQ=255) ccgacccTGTGGGAGCGGGCACGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGc > 1:371657/5‑140 (MQ=255) ccgacccTGTGGGAGCGGGCACGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGc < 2:371657/136‑1 (MQ=255) cTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGaa < 2:225014/99‑1 (MQ=255) cTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGaa > 1:225014/1‑99 (MQ=255) cTGTGGGAGCGGGCACGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATcgcg > 2:244149/1‑150 (MQ=255) tgtgGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCgcgc > 1:26405/1‑150 (MQ=255) gCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATc > 2:163368/1‑150 (MQ=255) cGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCg < 2:26405/150‑1 (MQ=255) | AAGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCG > NC_002947/6023076‑6023363 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |