| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 524,352 | G→C | 29.1% | intergenic (+216/‑600) | tyrS → / → PP_16SC | tyrosine‑‑tRNA ligase/16S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 524,352 | 0 | G | C | 29.1% | 35.3 / 15.1 | 24 | intergenic (+216/‑600) | tyrS/PP_16SC | tyrosine‑‑tRNA ligase/16S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base G (5/12); new base C (3/4); total (8/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGC > NC_002947/524216‑524487 | gttgtAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCCCGCGCGGCGCTc < 2:427749/149‑1 (MQ=255)gttgtAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCCCGCGCGGCGCTc > 1:427749/1‑149 (MQ=255)gttgtAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCCCGCGCGGCGCTCg > 1:206177/1‑150 (MQ=255) tgtAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCCCGCGCGGCGCTCGGt < 2:206177/150‑1 (MQ=255) gatAGGGGGTGGTGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGt < 1:446431/148‑1 (MQ=255) agggggCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCCCGCGCGGCGCTCGGTTTCATAGgc < 2:374748/150‑1 (MQ=255) gggCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCt < 1:179978/150‑1 (MQ=255) tGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTc > 2:256767/1‑150 (MQ=255) gTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCg < 2:175304/111‑1 (MQ=255) gTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCg > 1:175304/1‑111 (MQ=255) tCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCCCGCGCGGCGCTCGGTTTCATAGGCGGTGaatgt > 1:285927/1‑118 (MQ=25) tCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCCCGCGCGGCGCTCGGTTTCATAGGCGGTGaatgt < 2:285927/121‑4 (MQ=25) cacaACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAACCCGAGCGCCGCGCGGGCGGCGCGCGGTCTCATAGGCGCTGAAACTGTCGTCGCGAACACTTGGCAGCCTCGATACGGTc < 1:233592/150‑1 (MQ=255) acaACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCt < 2:264266/150‑1 (MQ=255) ccTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTcc < 1:108323/150‑1 (MQ=255) cTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTCAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTccc > 1:233172/1‑150 (MQ=255) cTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTc < 2:344421/150‑1 (MQ=255) gaTCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAAATGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTc < 1:256767/150‑1 (MQ=255) aTCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCt > 1:411062/1‑150 (MQ=255) cGGCTGGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTTTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGc < 2:37804/150‑1 (MQ=255) aTTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAgg < 1:37676/132‑1 (MQ=255) aTTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAgg > 2:37676/1‑132 (MQ=255) aTGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATg < 2:411062/150‑1 (MQ=255) aaaCCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATgcgc < 2:233172/150‑1 (MQ=255) | GTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGC > NC_002947/524216‑524487 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |