Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 868,054 G→T 12.2% intergenic (+149/+28) PP_0750 → / ← mqo‑I hypothetical protein/malate:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947868,0540GT12.2% 104.7 / 3.4 40intergenic (+149/+28)PP_0750/mqo‑Ihypothetical protein/malate:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (14/20);  new base T (3/2);  total (17/23)
Fisher's exact test for biased strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATCTTGTGGTACAGCGCAGCATCGGTTGCCAGGGTCTTCTCGCGAGCCGGGAA  >  NC_002947/867906‑868201
                                                                                                                                                    |                                                                                                                                                   
tCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGa                                                                                                                                                    >  2:160003/1‑150 (MQ=255)
 cTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAg                                                                                                                                                   >  2:269224/1‑150 (MQ=255)
   gTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAggg                                                                                                                                                 <  1:328102/150‑1 (MQ=255)
        cAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGtt                                                                                                                                            <  2:201604/150‑1 (MQ=255)
          gTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGtttt                                                                                                                                          >  2:382145/1‑150 (MQ=255)
             ccGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGTAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGc                                                                                                                                       <  2:213094/150‑1 (MQ=255)
                gCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAggg                                                                                                                                                 >  2:439513/1‑137 (MQ=255)
                gCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAggg                                                                                                                                                 <  1:439513/137‑1 (MQ=255)
                                          acaGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTg                                                                                                          <  1:241338/150‑1 (MQ=255)
                                                     gccgcACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGgct                                                                                               <  2:175612/150‑1 (MQ=255)
                                                      ccgcACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGgctg                                                                                              >  1:142500/1‑150 (MQ=255)
                                                                 ggCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAg                                                                                   <  1:239447/150‑1 (MQ=255)
                                                                 ggCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAg                                                                                   <  2:272450/150‑1 (MQ=255)
                                                                  gCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCTCGGGGCGttttttgt                                                                                                                                      <  2:233089/98‑4 (MQ=37)
                                                                  gCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCTCGGGGCGttttttgt                                                                                                                                      >  1:233089/1‑95 (MQ=255)
                                                                    aTTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTc                                                                                >  2:19499/1‑150 (MQ=255)
                                                                     ttACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTcc                                                                               >  1:421396/1‑150 (MQ=255)
                                                                        ccGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAgagatcgg                                                                            >  1:75098/1‑143 (MQ=255)
                                                                                gtgGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGcgtc                                                                    <  2:152110/150‑1 (MQ=255)
                                                                                     aGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTAt                                                               <  1:382145/150‑1 (MQ=255)
                                                                                             tGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGTCAGGTCCAGGGCCGCGTCGTTATCGGCACTg                                                       >  2:412619/1‑150 (MQ=255)
                                                                                                ccGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATc                                                    >  1:186649/1‑150 (MQ=255)
                                                                                                  gcgAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGTCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATCtt                                                  <  1:412619/150‑1 (MQ=255)
                                                                                                     aaGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATCTtgtg                                               <  2:421396/150‑1 (MQ=255)
                                                                                                               tACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATCTTGTGGTACAGCgca                                     <  2:6733/150‑1 (MQ=255)
                                                                                                                  aGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATCTTGTGGTACAGCgcagca                                  >  2:426434/1‑150 (MQ=255)
                                                                                                                      tGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGAt                                                     >  1:260608/1‑127 (MQ=255)
                                                                                                                      tGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGAt                                                     <  2:260608/127‑1 (MQ=255)
                                                                                                                       gATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATCTTGTGGTACAGCGCAGCATCGGt                             <  2:100842/150‑1 (MQ=255)
                                                                                                                       gATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATCTTGTGGTACAGCGCAGCATCGGt                             <  1:19499/150‑1 (MQ=255)
                                                                                                                       gATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCATTATAGACACTCATCTTGTGGTACAGCCCAGCATCCGt                             <  2:259519/150‑1 (MQ=255)
                                                                                                                          cAAGCGCCAGGCACAAAAAACGCCCCAAGGGGCGTTTGTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCATGGCCGCGTCGTTATCGGCACTGATCTTGTGGTACAGCGCAGCATCGGTTGc                          <  1:379435/150‑1 (MQ=255)
                                                                                                                            aGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGccgccg                                                                                                   <  2:80039/75‑1 (MQ=255)
                                                                                                                            aGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGccgccg                                                                                                   >  1:80039/1‑75 (MQ=255)
                                                                                                                                 cAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATCTTGTGGTACAGCGCAGCATCGGTTGCCAGGGtc                   <  1:269224/150‑1 (MQ=255)
                                                                                                                                     acgcaaaaaCGCCCCTCGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAg                                                                                   >  1:193496/5‑82 (MQ=38)
                                                                                                                                     acgcaaaaaCGCCCCTCGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAg                                                                                   <  2:193496/78‑1 (MQ=38)
                                                                                                                                         aaaaaCGCCCATCGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATCTTGTGGTACAGCGCAGCATCGGTTGCCAGGGTCTTCTcgcg           >  2:220823/1‑150 (MQ=255)
                                                                                                                                               gCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATCTTGTGGTACAGCGCAGCATCGGTTGCCAGGGTCTTCTCGCGAGCCgg     >  1:184534/1‑150 (MQ=255)
                                                                                                                                                  ccGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATCTTGTGGTACAGCGCAGCATCGGTTGCCAGGGTCTTCTCGCGAGCCGGGaa  <  1:139306/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATCTTGTGGTACAGCGCAGCATCGGTTGCCAGGGTCTTCTCGCGAGCCGGGAA  >  NC_002947/867906‑868201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑