Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,121,450 A→G 20.0% N296D (AAC→GAC)  PP_0982 → YjgP/YjgQ family permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,121,4500AG20.0% 47.6 / 10.0 30N296D (AAC→GAC) PP_0982YjgP/YjgQ family permease
Reads supporting (aligned to +/- strand):  ref base A (14/10);  new base G (3/3);  total (17/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.89e-01

CCGAGGAAGTGACCGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCTG  >  NC_002947/1121301‑1121593
                                                                                                                                                     |                                                                                                                                               
ccGAGGAAGTGACCGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCa                                                                                                                                                 >  2:351007/1‑150 (MQ=255)
      aaGTGACCGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTTGACCcgc                                                                                                                                           <  1:123829/150‑1 (MQ=255)
             cGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGGCGCGGGTTGACCcgcgaca                                                                                                                                       >  2:272428/1‑144 (MQ=255)
             cGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGGCGCGGGTTGACCCGCGACa                                                                                                                                       <  1:272428/147‑1 (MQ=255)
                    gAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTc                                                                                                                             <  2:43314/150‑1 (MQ=255)
                           ttCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAAGGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgc                                                                                                                      >  2:289366/1‑150 (MQ=255)
                               cACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgctgct                                                                                                                  <  1:373113/150‑1 (MQ=255)
                               cACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgctgct                                                                                                                  >  2:373113/1‑150 (MQ=255)
                                                     gaggGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAg                                                                                                                       <  2:26587/123‑1 (MQ=255)
                                                     gaggGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAg                                                                                                                       >  1:26587/1‑123 (MQ=255)
                                                         gCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTTGACCcgcga                                                                                                                                         >  2:381117/1‑100 (MQ=255)
                                                         gCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTTGACCcgcga                                                                                                                                         <  1:381117/101‑2 (MQ=255)
                                                                       gcCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTg                                                                          >  2:213708/1‑150 (MQ=255)
                                                                                  aTGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCTCGGGTAAACCCGCGCCAGGGGCGCTTCATCAAGCTGCTGCCGGCAATTCTTCTTTACATGGCCTACCTGACAATGCTGATTTCCGTAcg                                                               >  2:165523/1‑150 (MQ=255)
                                                                                            tCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTc                                                     <  1:351007/150‑1 (MQ=255)
                                                                                                tGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCGGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCgaga                                                 <  1:289366/150‑1 (MQ=255)
                                                                                                        cTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCaaa                                         >  2:150678/1‑150 (MQ=255)
                                                                                                         tGGTGTTCGTCGTCCCCCTGCTGGCAGTGCCGCTGTGGCGGGACAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTATACATAGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAGGGGAAAAc                                        >  2:43297/1‑150 (MQ=255)
                                                                                                         tGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAAttct                                                                                                      <  1:210163/88‑1 (MQ=255)
                                                                                                         tGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAAttct                                                                                                      >  2:210163/1‑88 (MQ=255)
                                                                                                               tcgtcgTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGa                                  <  2:414566/150‑1 (MQ=255)
                                                                                                                     tcACGCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCAGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGccc                            >  1:130965/1‑150 (MQ=255)
                                                                                                                         ccTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGACGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTggg                        >  1:299115/1‑150 (MQ=255)
                                                                                                                          ctgctgGCGGTTCCGGTGTCGCGGGTCAACCCGCGCCAGGGCCGCTCCCTCAAGCTTCTGCCGGCAATTCCTCTGTACAGGGCCAACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGc                       <  1:43297/150‑1 (MQ=255)
                                                                                                                           tgctgGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTAACAATGCTCAGTTCCGTACGCGGCGCCCTCGAGAAGGGCAGACTGCCGATCGCCCTGGTCa                      >  2:157608/1‑150 (MQ=255)
                                                                                                                              tgGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCAtgt                   <  1:213708/150‑1 (MQ=255)
                                                                                                                                   gTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTGGCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGtggtgg              <  1:357505/150‑1 (MQ=255)
                                                                                                                                     gCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGt            >  1:152593/1‑150 (MQ=255)
                                                                                                                                         cTGGCGCGGGTTGACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCAc        >  1:33195/1‑150 (MQ=255)
                                                                                                                                              gcgGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCt   >  2:240896/1‑150 (MQ=255)
                                                                                                                                               cgGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCTg  >  1:220282/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
CCGAGGAAGTGACCGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCTG  >  NC_002947/1121301‑1121593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑