Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,130,341 G→C 23.5% H101D (CAC→GAC)  PP_0990 ← DoxX family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,130,3410GC23.5% 28.1 / 4.1 17H101D (CAC→GAC) PP_0990DoxX family protein
Reads supporting (aligned to +/- strand):  ref base G (8/5);  new base C (2/2);  total (10/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

AAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCG‑CCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAGGTGGGTGGGCCACGCATCGGGGTAGACGAACACTTCGATCACCAACGTCATGCCCAGCAGTGCCAGCGCCGATAGCCGGGTCATCAGCCCGAGCACCAGCAATAGCGGGAACAAGTGCTCAGCGTAGGCGGCCAGGTGCGCTGCAAGCCA  >  NC_002947/1130206‑1130488
                                                                                                                                        |                                                                                                                                                   
aaaaCGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGAAGCACTGGTGGAAAGGCGCCGGCG‑CCTCGGGCAACCAGGAGCAGCATGAGCCCTGCCCAGGGCAGGTGGGTGGGCCACGCAt                                                                                                                                       >  2:238658/1‑150 (MQ=255)
             tggtgCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCG‑CCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAGGTGGGTGGGCCACGCATCGGGGTAGACGaa                                                                                                                          <  2:249811/150‑1 (MQ=255)
              ggtgCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCG‑CCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAGGTGGGTGGGCCAGAAGTCGGGGTAGACGAac                                                                                                                         <  1:238658/150‑1 (MQ=255)
                  ccTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCG‑CCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAGGTGGGTGGGCCACGCATCGGGGTAGACGAACACtt                                                                                                                     >  1:64157/1‑150 (MQ=255)
                             gAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCCGATGATCCAGCGAAAACGCGCCGGCG‑CCCCCGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGACAGGTGGCTGGGCCACTCCTGGGGGTAGCCGCACCCTTCCATCTCCAAc                                                                                                          <  2:129898/150‑1 (MQ=255)
                                     aaTGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCG‑CCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAGGTCGGTGGGCCACGCATCGGGGTAGACGAACACTTCGATCACCAACGTCATGcc                                                                                                  >  2:336208/1‑150 (MQ=255)
                                     aaTGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCG‑CCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAGGTCGGTGGGCCACGCATCGGGGTAGACGAACACTTCGATCACCAACGTCATGcc                                                                                                  >  2:335995/1‑150 (MQ=255)
                                                gTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCG‑CCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAGGTCGGTGGGCCACGCATCGGGGTAGACGAACACTTCGATCACCAACGTCATGCCCAGCAGTGCCa                                                                                       <  1:335995/150‑1 (MQ=255)
                                                gTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCG‑CCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAGGTCGGTGGGCCACGCATCGGGGTAGACGAACACTTCGATCACCAACGTCATGCCCAGCAGTGCCa                                                                                       <  1:336208/150‑1 (MQ=255)
                                                          tttCAGCAGATGATCCAGCGAAAACGCGCCGGCG‑CCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAGGTGGGTGGGCCACGCATCGGGGTAGACGAACACTTCGATCACCAACGTCATGCCCAGCAGTGCCAGCGCCGATAg                                                                             >  1:287192/1‑150 (MQ=255)
                                                                         cAGCGAAAACGCGCCGGCG‑CCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAGGTGGGTGGGCCACGCATCGGGGTAGACGAACACTTCGATCACCAACGTCATGCCCAGCAGTGCCAGCGCCGATAGCCGGGTCATCAGccc                                                              <  1:95965/150‑1 (MQ=255)
                                                                            ccgttgcgcgCCGGCGTACTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAGGTGGGTGGGCCACGCATCGGGGTAGACGAACACTTCGATCACCAACGTCATGCCCAGCAGTGCCAGCGCCGATAGCCgg                                                                         >  2:2280/7‑137 (MQ=255)
                                                                            ccgttgcgcgCCGGCGTACTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAGGTGGGTGGGCCACGCATCGGGGTAGACGAACACTTCGATCACCAACGTCATGCCCAGCAGTGCCAGCGCCGATAGCCgg                                                                         <  1:2280/131‑1 (MQ=255)
                                                                              aaaaCGCGCCGGCG‑CCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAGGTGGGTGGGCCACGCATCGGGGTAGACGAACACTTCGATCACCAACGTCATGCCCAGCAGTGCCAGCGCCGATAGCCGGGTCATCAGCCCGAGCa                                                         >  1:25397/1‑150 (MQ=255)
                                                                                      ccGGCG‑CCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAGGTGGGTGGGCCACGCATCGGGGTAGACGAACACTTCGATCACCAACGTCATGCCCAGCAGTGCCAGCGCCGATAGCCGGGTCATCAGCCCGAGCACCAGCAAt                                                 >  1:13547/1‑150 (MQ=255)
                                                                                                                       cccTGCCCAGGTCAGGTGGGTGGGCCACGCATCGGGGTAGACGAACACTTCGATCACCAACGTCATGCCCAGCAGTGCCAGCGCCGATAGCCGGGTCATCAGCCCGAGCACCAGCAATAGCGGGAACAAGTGCTCAGCGTAGGCGGCCAg                 >  1:251689/1‑150 (MQ=255)
                                                                                                                        ccTGCCCAGGTCAGGTGGGTGGGCCACGCATCGGGGTAGACGAACACTTCGATCACAAACCTCATGCCCAGCATTTCCAGCGCCGATAGCCGGGTCATCAGCCCGAGCACCAGCAATAGCGGGAACAAGTGCTCAGCGTAGGCGGCCAgg                >  1:166917/1‑150 (MQ=255)
                                                                                                                                      gtgggtggGCCACGCATCGGGGTAGACGAACACTTCGATCACCAACGTCATGCCCAGCAGTGCCAGCGCCGATAGCCGGGTCATCAGCCCGAGCACCAGCAATAGCGGGAACAAGTGCTCAGCGTAGGCGGCCAGGTGCGCTGCAAGCCa  >  1:85367/1‑150 (MQ=255)
                                                                                                                                        |                                                                                                                                                   
AAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCG‑CCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAGGTGGGTGGGCCACGCATCGGGGTAGACGAACACTTCGATCACCAACGTCATGCCCAGCAGTGCCAGCGCCGATAGCCGGGTCATCAGCCCGAGCACCAGCAATAGCGGGAACAAGTGCTCAGCGTAGGCGGCCAGGTGCGCTGCAAGCCA  >  NC_002947/1130206‑1130488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑