| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,196,957 | G→A | 21.0% | A338T (GCC→ACC) | xcpQ → | type II secretion pathway protein XcpQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,196,957 | 0 | G | A | 21.0% | 30.7 / 4.3 | 19 | A338T (GCC→ACC) | xcpQ | type II secretion pathway protein XcpQ |
| Reads supporting (aligned to +/- strand): ref base G (8/7); new base A (2/2); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01 | |||||||||||
TGGTGGTCGAAGCGATCATCGCCGAGGTGTCCGACAGCAGCGCCCAGGAGCTGGGCGTGCAGTGGCTGTTCGCCGACGAGAAGTTCGGCGCCGGTATCGTCAACTTCGGCAGTAACGGGGTGAATATCGCCAGCATCGCCGGCGCCGCCGCCAGTGGCGACAACGAGGCCCTGGGCGATCTGTTGTCCACCACCACCGGTGCCACGGCGGGCATCGGCCATTTCGGCGGTGGCTTCAACTTCGCCATGCTGGTCAACGCGCTGAAGGGCAAGAGCGGCTTCAACCTGCTGTC > NC_002947/1196808‑1197099 | tggtggtCGAAGCGATCATCGCCGAGGTGTCCGACAGCAGCGCCCAGGAGCTGGGCGTGCAGTGGCTGTTCGCCGACGAGAAGTTCGGCGCCGGTATCGTCAACTTCGGCAGTAACGGGGTGAATATCGCCAGCATCGCCGGcgccgccg > 2:232532/1‑150 (MQ=255) gtCGAAGCGATCATCGCCGAGGTGTCCGACAGCAGCGCCCAGGAGCTGGGCGTGCAGTGGCTGTTCGCCGACGAGAAGTTCGGCGCCGGTATCGTCAACTTCGGCAGTAACGGGGTGAATATCGCCAGCATCGCCGGCGCCGCCGCCAGt < 2:137695/150‑1 (MQ=255) tCGAAGCGATCATCGCCGAGGTGTCCGACAGCAGCGCCCAGGAGCTGGGCGTGCAGTGGCTGTTCGCCGACGAGAAGTTCGGCGCCGGTATCGTCAACTTCGGCAGTAACGGGGTGAATATCGCCAGCATCGCCGGCGCCGCCGCCAGTg < 1:307825/150‑1 (MQ=255) tgtCCGACAGCAGCGCCCAGGAGCTGGGCGTGCAGTGGCTGTTCGCCGACGAGAAGTTCGGCGCCGGTATCGTCAACTTCGGCAGTAACGGTGTGAATATCGCCAGCATCGCCGGCGCCGCCGCCAGTGGCGACAAc < 1:300201/137‑1 (MQ=255) tgtCCGACAGCAGCGCCCAGGAGCTGGGCGTGCAGTGGCTGTTCGCCGACGAGAAGTTCGGCGCCGGTATCGTCAACTTCGGCAGTAACGGGGTGAATATCGCCAGCATCGCCGGCGCCGCCGCCAGTGGCGACAAc > 2:300201/1‑137 (MQ=255) gcagcGCCCAGGAGCTGGGCGTGCAGTGGCTGTTCGCCGACGAGAAGTTCGGCGCCGGTATCGTCAACTTCGGCAGTAACGGGGTGAATATCGCCAGCATCGCCGGCGCCGCCGCCAGTGGCGACAACGAGGCCCTGGGCGATCtgttgt > 2:155464/1‑150 (MQ=255) agcGCCCAGGAGCTGGGCGTGCAGTGGCTGTCCGCCGACGAGAAGTTCGGCGCCGGTATCGTCAACTTCGGCAGTAACGGGGTGAATATCGCCAGCATCGCCGGCGCCGCCGCCAGTGGCGACAACGAGGACCTGGGCGATCTGTTGTcc < 1:106310/150‑1 (MQ=255) cccAGGAGCTGGGCGTGCAGTGGCTGTTCGCCGACGAGAAGTTCGGCGCCGGTATCGTCAACTTCGGCAGTAACGGGGTGAATATCGCCAGCATCGCCGGCGCCGCCGCCAGTGGCGACAACGAGGCCCTGGGCGATCTGTTGTccacca > 1:322539/1‑150 (MQ=255) tGGCTGTTCGCCGACGAGAAGTTCGGCGCCGGTATCGTCAACTTCGGCAGTAACGGGGTGAATATCGCCAGCATCGCCGGCGCCGCCGCCAGTGGCGACAACGAGGCCCTGGGCGATCTGTTGTCCACCACCACCGGTGCCACGGCGGGc > 2:130405/1‑150 (MQ=255) gCTGTTCGCCGACGAGAAGTTCGGCGCCGGTATCGTCAACTTCGGCAGTAACTGGGTGAATATCGCCAGCATCGCCGGCGCCGCCGCCAGTGGCGACAACGAGGCCCTGGGCGATCTGTTGTCCACCACCACCGGTGCCACGGCGGGCAt > 1:123029/1‑150 (MQ=255) cGCCGACGAGAAGTTCGGCGCCGGTATCGTCAACTTCGGCAGTAACGGGGTTAATACCGCCAGCATCGCCGGCGCCGCCGCCAGTGGCGACAACGAGGCCCTGGGCGATCTGTTGTCCACCACCACCGGTGCCACGGCGGGCATCGGCCa < 1:14896/150‑1 (MQ=255) gacgaGAAGTTCGGCGCCGGTATCGTCAACTTCGGCAGTAACGGGGTGAATATCGCCAGCATCGCCGGCGCCGCCGCCAGTGGCGACAACGAGGCCCTGGGCGATCTGTTGTCCACCACCACCGGTGCCACGGCGGGCATCGGCCATTTc > 2:279643/1‑150 (MQ=255) gaAGTTCGGCGCCGGTATCGTCAACTTCGGCAGTAACGGGGTGAATATCGCCAGCATCGCCGGCGCCGCCACCAGTGGCGACAACGAGGCCCTGGGCGATCTGTTGTCCACCACCACCGGTGCCACGGCGGGCATCGGc > 1:155989/1‑139 (MQ=255) gaAGTTCGGCGCCGGTATCGTCAACTTCGGCAGTAACGGGGTGAATATCGCCAGCATCGCCGGCGCCGCCACCAGTGGCGACAACGAGGCCCTGGGCGATCTGTTGTCCACCACCACCGGTGCCACGGCGGGCATCGGc > 1:155893/1‑139 (MQ=255) gaAGTTCGGCGCCGGTATCGTCAACTTCGGCAGTAACGGGGTGAATATCGCCAGCATCGCCGGCGCCGCCACCAGTGGCGACAACGAGGCCCTGGGCGATCTGTTGTCCACCACCACCGGTGCCACGGCGGGCATCGGc < 2:155893/139‑1 (MQ=255) gaAGTTCGGCGCCGGTATCGTCAACTTCGGCAGTAACGGGGTGAATATCGCCAGCATCGCCGGCGCCGCCACCAGTGGCGACAACGAGGCCCTGGGCGATCTGTTGTCCACCACCACCGGTGCCACGGCGGGCATCGGc < 2:155989/139‑1 (MQ=255) ggCGCCGGTATCGTCAACTTCGGCAGTAACGGGGTGAATATCGCCAGCATCGCCGGCGCCGCCGCCAGTGGCGACAACGAGGCCCTGGGCGATCTGTTGTCCACCACCACCGGTGCCACGGCGGGCATCGGCCATTTCGGCGGTGGCTTc < 1:279643/150‑1 (MQ=255) gcCGGTATCGTCAACTTCGGCAGTAACGGGGTGAATATCGCCAGCATCGCCGGCGCCGCCGCCAGTGGCGACAACGAGGCCCTGGGCGATCTGTTGTCCACCACCACCGGTGCCACGGCGGGCATCGGCCATTTCGGCGGTGGCTTCAAc < 1:130405/150‑1 (MQ=255) gTGAATATCGCCAGCATCGCCGGCGCCGCCGCCAGTGGCGACAACGAGGCCCTGGGCGATCTGTTGTCCACCACCACCGGTGCCACGGCGGGCATCGGCCATTTCGGCGGTGGCTTCAACTTCGCCATGCTGGTCAACGCGCTGAAGGGc > 2:326874/1‑150 (MQ=255) tGAATATCGCCAGCATCGCCGGCGCCGCCGCCAGTGGCGACAACGAGGCCCTGGGCGATCTGTTGTCCACCACCACCGGTGCCACGGCGGGCATCGGCCATTTCGGCGGTGGCTTCAACTTCGCCATGCTGGTCAACGCGCTGAAGGGCa > 1:268437/1‑150 (MQ=255) cgccgccgcCAGTGGCGACAACGAGGCCCTGGGCGATCTGTTGTCCACCACCACCGGTGCCACGGCGGGCATCGGCCATTTCGGCGGTGGCTTCAACTTCGCCATGCTGGTCAACGCGCTGAAGGGCAAGAGCGGCTTCAACCTGCTGTc < 2:322539/150‑1 (MQ=255) gccgccgccAGTGGCGACAACGAGGCCCTGGGCGATCTGTTGTCCACCACCACCGGTGCCACGGCGGGCATCGGCCATTTCGGCGGTGGCTTCAACTTCGCCATGCTGGTCAACGCGCTGAAGGGCAAGAGCGGc < 2:415079/135‑1 (MQ=255) gccgccgccAGTGGCGACAACGAGGCCCTGGGCGATCTGTTGTCCACCACCACCGGTGCCACGGCGGGCATCGGCCATTTCGGCGGTGGCTTCAACTTCGCCATGCTGGTCAACGCGCTGAAGGGCAAGAGCGGc > 1:415079/1‑135 (MQ=255) | TGGTGGTCGAAGCGATCATCGCCGAGGTGTCCGACAGCAGCGCCCAGGAGCTGGGCGTGCAGTGGCTGTTCGCCGACGAGAAGTTCGGCGCCGGTATCGTCAACTTCGGCAGTAACGGGGTGAATATCGCCAGCATCGCCGGCGCCGCCGCCAGTGGCGACAACGAGGCCCTGGGCGATCTGTTGTCCACCACCACCGGTGCCACGGCGGGCATCGGCCATTTCGGCGGTGGCTTCAACTTCGCCATGCTGGTCAACGCGCTGAAGGGCAAGAGCGGCTTCAACCTGCTGTC > NC_002947/1196808‑1197099 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |