| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,955,253 | T→A | 20.0% | intergenic (+40/‑153) | PP_1752 → / → PP_1753 | hypothetical protein/DMT superfamily permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,955,253 | 0 | T | A | 20.0% | 50.3 / 9.3 | 25 | intergenic (+40/‑153) | PP_1752/PP_1753 | hypothetical protein/DMT superfamily permease |
| Reads supporting (aligned to +/- strand): ref base T (8/12); new base A (2/3); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.87e-01 | |||||||||||
CTTGGACGCTGGTGGAGGCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACCACGGGCTTGATCGAG > NC_002947/1955110‑1955399 | cTTGGACGCTGGTGGAGGCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCcgc > 1:265886/1‑150 (MQ=255) ggACGCTGGTGGAGGCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTAAACCCGCGaa < 1:2052/150‑1 (MQ=255) cGCTGGTGGAGGCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAgg < 1:265378/150‑1 (MQ=255) gtggAGGCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAg > 2:336385/1‑150 (MQ=255) cAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGt > 2:370224/1‑139 (MQ=255) cAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGt < 1:370224/139‑1 (MQ=255) cAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCt < 2:107926/150‑1 (MQ=255) aaGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTc < 1:336385/150‑1 (MQ=255) aaGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTGCTGACCCTTTCGCGGGTAAACCCGCGaagcggcc > 2:88401/1‑127 (MQ=25) aaGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTGCTGACCCTTTCGCGGGTAAACCCGCGaagcggcc < 1:88401/133‑7 (MQ=37) ggTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGcc < 2:179290/150‑1 (MQ=255) gcgcACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGa < 1:158149/150‑1 (MQ=255) gcACAATCAACCAGGCGCTTGAGCCGGCGTTTGCCAAGCACAAGAAATGGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGAcc < 2:28122/150‑1 (MQ=255) gcTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGCATGACCTGATGAATCCTTTtct < 2:340738/150‑1 (MQ=255) cAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGcc > 2:254580/1‑102 (MQ=255) cAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGcc < 1:254580/102‑1 (MQ=255) aaGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGtctc > 2:268206/1‑150 (MQ=255) aGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTctct < 2:265886/150‑1 (MQ=255) aGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTca > 1:445499/1‑150 (MQ=255) gCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACCACGGGc > 2:379936/1‑150 (MQ=255) tttCGCGGGTAAACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTa > 1:393336/2‑124 (MQ=255) tttCGCGGGTAAACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTa < 2:393336/123‑1 (MQ=255) cgcgGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACCACGGGCTTGa < 1:268206/150‑1 (MQ=255) ggTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACCACGGGCTTGATCGa < 2:196077/150‑1 (MQ=255) gTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACCACGGGCTTGATCGAg > 2:353310/1‑150 (MQ=255) | CTTGGACGCTGGTGGAGGCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACCACGGGCTTGATCGAG > NC_002947/1955110‑1955399 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |