Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,851,762	C→T	S269L (TCG→TTG)	relA →	ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,851,762	0	C	T	100.0%	148.0 / NA	46	S269L (TCG→TTG)	relA	ATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/23);  total (23/23)

CAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGT  >  NC_002947/1851617‑1851908                                                                                                                                                   |                                                                                                                                                   cAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATc                                                                                                                                                <  2:1129180/150‑1 (MQ=255)      cAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGgcg                                                                                                                                           >  1:941591/1‑150 (MQ=255)       aaGCAGATCGCCAAGCTGTTGCATGAGCTACTGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGgcgc                                                                                                                                          >  1:1044993/1‑150 (MQ=255)         gCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCaa                                                                                                                                        <  2:653102/150‑1 (MQ=255)         gCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGAACTGCAACAACGACGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCaa                                                                                                                                        <  2:40787/150‑1 (MQ=255)            gATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGAt                                                                                                                                     >  2:250148/1‑150 (MQ=255)                 ccAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAgc                                                                                                                                >  1:1299701/1‑150 (MQ=255)                 ccAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCACAAGCAACGCCACGCACGCGCTTGCGGGTTAGT‑TGCTAGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAgc                                                                                                                                <  1:496618/150‑1 (MQ=255)                         ttGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGc                                                                                                                        <  2:288196/150‑1 (MQ=255)                            cATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTg                                                                                                                     <  1:121530/150‑1 (MQ=255)                            cATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTg                                                                                                                     >  1:1005288/1‑150 (MQ=255)                             aTGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTgg                                                                                                                    <  2:1005288/150‑1 (MQ=255)                               gAGCGGCGGCTGGCCCTCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAAAGCTTGTGCT‑ACCTTTGGTGGGGAGGCCGACATCAGCGGCCCGGCGCAACATCTCTATTTCAGCTGGCGCAAGATGCAGCGCAAAGGCCTGGAg                                                                                                                  <  1:1493629/150‑1 (MQ=255)                               gAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAg                                                                                                                  >  2:1281475/1‑150 (MQ=255)                                  cggcggCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGatcca                                                                                                               <  2:668013/150‑5 (MQ=255)                                             ccGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTa                                                                                                    <  2:468059/150‑1 (MQ=255)                                                   gcgcTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgt                                                                                              <  2:249022/150‑1 (MQ=255)                                                     gcTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtgc                                                                                            >  2:904649/1‑150 (MQ=255)                                                       ttCATCAGCGACGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCGGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtgctt                                                                                          >  2:616746/1‑148 (MQ=255)                                                               cGACGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCa                                                                                                          <  1:274614/126‑1 (MQ=255)                                                               cGACGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCa                                                                                                          >  2:274614/1‑126 (MQ=255)                                                                 aCGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGcgcg                                                                                <  1:250148/150‑1 (MQ=255)                                                                    tgatgaACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGc                                                                             <  1:92405/150‑1 (MQ=255)                                                                     gatgaACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGa                                                                                                        >  2:462741/1‑122 (MQ=255)                                                                     gatgaACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGa                                                                                                        <  1:462741/122‑1 (MQ=255)                                                                      atgaACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTg                                                                           <  2:155499/150‑1 (MQ=255)                                                                       tgaACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTgg                                                                          >  1:1216290/1‑150 (MQ=255)                                                                             aGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCgg                                                                    <  1:545798/150‑1 (MQ=255)                                                                                 gCAGACCGAGT‑TGATCGCCACTGGCGTGAAGGCCGACATGAGCAGCAGGGCGAAACATAGCTATGTGCAGATGTGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAAt                                                                <  1:416662/150‑1 (MQ=255)                                                                                    gAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGaaa                                                                 >  2:921933/1‑146 (MQ=255)                                                                                    gAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGaaa                                                                 <  1:921933/146‑1 (MQ=255)                                                                                     aaCGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCcgc                                                            >  2:368545/1‑150 (MQ=255)                                                                                          gT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCTACTg                                                       >  2:229236/1‑150 (MQ=255)                                                                                          gT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTg                                                       >  2:1240112/1‑150 (MQ=255)                                                                                              gCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTa                                                                                                    >  1:821976/1‑102 (MQ=255)                                                                                              gCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTa                                                                                                    <  2:821976/102‑1 (MQ=255)                                                                                                tCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTaca                                                  >  1:556319/1‑150 (MQ=255)                                                                                                tCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTaca                                                  >  1:1278815/1‑150 (MQ=255)                                                                                                  gCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACAcc                                                >  2:513021/1‑150 (MQ=255)                                                                                                  gCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACAcc                                                >  1:356621/1‑150 (MQ=255)                                                                                                        ggCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTg                                          <  1:1281475/150‑1 (MQ=255)                                                                                                         gCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTgg                                         <  1:1046404/150‑1 (MQ=255)                                                                                                                                        aCATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTcc          <  2:1278815/150‑1 (MQ=255)                                                                                                                                        aCATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTcc          >  1:767553/1‑150 (MQ=255)                                                                                                                                             tCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAgg     >  2:185571/1‑150 (MQ=255)                                                                                                                                                aTTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGt  >  2:619840/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGT‑TGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGT  >  NC_002947/1851617‑1851908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑