Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	181,805	T→C	18.8%	intergenic (+19/‑87)	PP_r01 → / → PP_5SB	5S ribosomal RNA/5S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	181,805	0	T	C	18.8%	59.3 / 9.8	32	intergenic (+19/‑87)	PP_r01/PP_5SB	5S ribosomal RNA/5S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (15/11);  new base C (4/2);  total (19/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01

GAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAAC  >  NC_002947/181663‑181951                                                                                                                                               |                                                                                                                                                   gAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGcaca                                                                                                                                             >  1:126941/1‑150 (MQ=32)                         ttGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCATATCTGCACATGCAGGTAGGGGTTTTGTCTTTaa                                                                                                                     <  1:204448/150‑1 (MQ=17)                                       tcccatcccGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACaga                                                                                                       <  2:126941/150‑1 (MQ=40)                                             cccGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCt                                                                                                                         <  2:489278/126‑1 (MQ=33)                                             cccGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCt                                                                                                                         >  1:489278/1‑126 (MQ=33)                                                 aaCTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTCTCTGCACACGCAGGTAGGGGTTTTTTCTGTAAGTAGGAACTACAGAGATTCGCAgg                                                                                             <  1:62132/150‑1 (MQ=255)                                                      agtagtGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGt                                                                                        >  2:46000/1‑150 (MQ=255)                                                           tGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAg                                                                                   <  2:393935/150‑1 (MQ=255)                                                             aaaCGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAgaga                                                                                                     <  1:456248/130‑1 (MQ=255)                                                             aaaCGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAgaga                                                                                                     >  2:456248/1‑130 (MQ=255)                                                              aaCGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGa                                                                                >  1:449753/1‑150 (MQ=255)                                                                 gATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAGAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACgg                                                                             <  2:206425/150‑1 (MQ=255)                                                                            gATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGcacaca                                                                  >  2:390508/1‑150 (MQ=255)                                                                             aTGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTaa                                                                                                                     >  2:310716/1‑98 (MQ=34)                                                                             aTGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTaa                                                                                                                     <  1:310716/98‑1 (MQ=34)                                                                                        gggTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCGATCTGCACATGCAGGGAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGCGCCAGCACACAGAATTTCTTgac                                                      >  2:375989/1‑150 (MQ=255)                                                                                                     tgAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCAt                                         >  1:44596/1‑150 (MQ=255)                                                                                                      gagaGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCAtt                                        <  1:390508/150‑1 (MQ=255)                                                                                                                  tCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGa                            >  2:316871/1‑150 (MQ=255)                                                                                                                    gTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGatc                          >  1:211269/1‑150 (MQ=255)                                                                                                                          aTTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTgacga                                                    >  2:484270/1‑118 (MQ=255)                                                                                                                          aTTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTgacga                                                    <  1:484270/118‑1 (MQ=255)                                                                                                                             ttaaagacAAAACCCCTACCTGCATGTGCAGATAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACCGGCCACCATACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGa                 >  2:67305/8‑150 (MQ=25)                                                                                                                               tttCGCAAAACCACTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAAc               >  1:40716/1‑150 (MQ=255)                                                                                                                                   acAAAACCCCTACCTGCATGTGCAGATAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGaa                                                               <  2:324029/97‑1 (MQ=37)                                                                                                                                   acAAAACCCCTACCTGCATGTGCAGATAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGaa                                                               >  1:324029/2‑98 (MQ=37)                                                                                                                                   acAAAACCCCTACCTGCATGTGCAGATAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGa                                     <  2:76012/123‑1 (MQ=38)                                                                                                                                   acAAAACCCCTACCTGCATGTGCAGATAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGa                                     >  1:76012/2‑124 (MQ=38)                                                                                                                                      aaaCCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTcagcag        >  2:170165/1‑150 (MQ=255)                                                                                                                                        aCCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTg      <  1:46000/150‑1 (MQ=255)                                                                                                                                          cccTACCTGCATGTGCAGATAGGGGTTTTGTCTTTAAGTAGGAAATACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGaa    >  2:242618/1‑150 (MQ=37)                                                                                                                                            cTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAAc  >  2:7482/1‑150 (MQ=255)                                                                                                                                               |                                                                                                                                                   GAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAAC  >  NC_002947/181663‑181951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑