| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,350,711 | G→A | 16.0% | G389S (GGC→AGC) | PP_2945 → | two‑component system sensor histidine kinase/response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,350,711 | 0 | G | A | 16.0% | 54.0 / 4.1 | 25 | G389S (GGC→AGC) | PP_2945 | two‑component system sensor histidine kinase/response regulator |
| Reads supporting (aligned to +/- strand): ref base G (11/10); new base A (2/2); total (13/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
AAGCCGACCTGGTAAAACGGTACCAGCCGTTACCCCCTGTCGAATGCCTGCCGTCGCAGATCAACCAAGTGATAATGAACCTGGTGCTCAACGCCGCCCAAGCCATGGGCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAGGCGCCTGGGTGTGCATCGAAGTTGCAGATACAGGCGCGGGGATAGCACCGGAGAACATCAAGAAAATCTTCGATCCTTTTTTCACCACCAAGCCCGTCGGCCAAGGTACCGGGCTAGGCCTGTCCCTTTCCTATGGCATTGTAAAGA > NC_002947/3350562‑3350858 | aaGCCGACCTGGTAAAACGGTACCAGCCGTTACCCCCTGTCGAATGCCTGCCGTCGCAGATCAACCAAGTGATAATGAACCTGGTGCTCAACGCCGCCCAAGCCATGGGCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAg < 2:344027/150‑1 (MQ=255) aaaaCGGTACCAGCCGTTACCCCCTGTCGAATGCCTGCCGTCGCAGATCAACCAAGTGATAATGAACCTGGTGCTCAACGCCGCCCAAGCCATGGGCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAGGCGCCTGGGtgtg < 1:272112/150‑1 (MQ=255) tACCAGCCGTTACCCCCTGTCGAATGCCTGCCGTCGCAGATCAACCAAGTGATAATGAACCTGGTGCTCAACGCCGCCCAAGCCATGGGCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAGGCGCCTGGGTGTGCATCGaa > 1:103759/1‑150 (MQ=255) gCCGTTACCCCCTGTCGAATGCCTGCCGTCGCAGATCAACCAAGTGATAATGAACCTGGTGCTCAACGCCGCCCAAGCCATGGGCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAGGCGCCTGGGTGTGCATCGAAGTTGc > 2:444328/1‑150 (MQ=255) aCCCCCTGTCGAATGCCTGCCGTCGCAGATCAACCAAGTGATAATGAACCTGGTGCTCAACGCCGCCCAAGCCATGGGCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAGGCGCCTGGGTGTGCATCGAAGTTGCAGATAc > 1:44087/1‑150 (MQ=255) cccTGTCGAATGCCTGCCGTCGCAGATCAACCAAGTGATAATGAACCTGGTGCTCAACGCCGCCCAAGCCATGGGCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAGGCGCCTGGgt > 1:215802/1‑126 (MQ=255) cccTGTCGAATGCCTGCCGTCGCAGATCAACCAAGTGATAATGAACCTGGTGCTCAACGCCGCCCAAGCCATGGGCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAGGCGCCTGGgt < 2:215802/126‑1 (MQ=255) gTCGCAGATCAACCAAGTGATAATGAACCTGGTGCTCAACGCCGCCCAAGCCATGGGCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAGGCGCCTGGGTGTGCATCGAAGTTGCAGATACAGGCGCGGGGATAGCACCGga < 1:444328/150‑1 (MQ=255) gCAGATCAACCAAGTGATAATGAACCTGGTGCTCAACGCCGCCCAAGCCATGGGCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAAGCGCCt < 1:309252/101‑1 (MQ=255) gCAGATCAACCAAGTGATAATGAACCTGGTGCTCAACGCCGCCCAAGCCATGGGCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAAGCGCCt < 1:309655/101‑1 (MQ=255) gCAGATCAACCAAGTGATAATGAACCTGGTGCTCAACGCCGCCCAAGCCATGGGCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAAGCGCCt > 2:309655/1‑101 (MQ=255) gCAGATCAACCAAGTGATAATGAACCTGGTGCTCAACGCCGCCCAAGCCATGGGCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAAGCGCCt > 2:309252/1‑101 (MQ=255) accAAGCCATGGGCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAGGCGCCTGGGTGTGCATCGAAGTTGCAGATACAGGCGCGGGGATAGCACCGGAGAACATCAAGAAAATCTTCGATCCTTTTTTCACCACCAAGCCCg > 2:477129/2‑150 (MQ=255) cAAGCCATGGGCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAGGCGCCTGGGTGTGCATCGAAGTTGCAGATACAGGCGCGGGGATAGCACCGGAGAACATCAAGAAAATCTTCGATCCTTTTTTCACCACCAAGCCCGTc > 2:316897/1‑150 (MQ=255) aTGGGCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAGGCGCCTGGGTGTGCATCGAAGTTGCAGATACAGGCGCGGGGATAGCACCGGAGAACATCAAGAAAATCTTCGATCCTTTTTTCACCACCAAGCCCGTCGGCCaa < 2:304192/150‑1 (MQ=255) tGGGCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAGGCGCCTGGGTGTGCATCGAAGTTGCAGATACAGGCGCGGGGATAGCACCGGAGAACATCAAGAAAATCTTCGATCCTTTTTTCACCACCaa > 2:223322/1‑136 (MQ=255) tGGGCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAGGCGCCTGGGTGTGCATCGAAGTTGCAGATACAGGCGCGGGGATAGCACCGGAGAACATCAAGAAAATCTTCGATCCTTTTTTCACCACCaa < 1:223322/136‑1 (MQ=255) gggCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAGGCGCCTGGGTGTGCATCGAAGTTGCAGATACAGGCGCGGGGATAGCACCGGAGAACATCAAGAAAATCTTCGATCCtttttt < 2:294470/126‑1 (MQ=255) gggCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAGGCGCCTGGGTGTGCATCGAAGTTGCAGATACAGGCGCGGGGATAGCACCGGAGAACATCAAGAAAATCTTCGATCCtttttt > 1:294470/1‑126 (MQ=255) gggCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAGGCGCCTGGGTGTGCATCGAAGTTGCAGATACAGGCGCGGGGATAGCACCGGAGAACATCAAGAAAATCTTCGATCCTTTTTTCACCACCAAGCCCGTCGGCCAAgg > 1:55861/1‑150 (MQ=255) cATCACATTGGGCACTGCTCATCAAGGCGCCTGGGTGTGCATCGAAGTTGCAGATACAGGCGCGGGGATAGCACCGgaga < 2:227809/80‑1 (MQ=255) cATCACATTGGGCACTGCTCATCAAGGCGCCTGGGTGTGCATCGAAGTTGCAGATACAGGCGCGGGGATAGCACCGgaga > 1:227809/1‑80 (MQ=255) cATCACATTGGGCACTGCTCATCAAGGCGCCTGGGTGTGCATCGAAGTTGCAGATACAGGCGCGGGGATAGCACCGGAGAACATCAAGAAAATCTTCGATCCTTTTGTTATTATCATTTCCGTCGGCCAAGGTACCGGGCTAGGCCTGTc > 2:424403/1‑150 (MQ=255) acaTTGGGCACTGCTCATCAAGGCGCCTGGGTGTGCATCGAAGTTGCAGATACAGGCGCGGGGATAGCACCGGAGAACATCAAGAAAATCTTCGATCCTTTTTTCACCACCAAGCCCGTCGGCCAAGGTACCGGGCTAGGCCTGTCCCtt < 2:292184/150‑1 (MQ=255) ggCACTGCTCATCAAGGCGCCTGGGTGTGCATCGAAGTTGCAGATACAGGCGCGGGGATAGCACCGGAGAACATCAAGAAAATCTTCGATCCTTTTTTCACCACCAAGCCCGTCGGCCAAGGTACCGGGCTAGGCCTGTCCCTTTCCTAt < 1:316897/150‑1 (MQ=255) aaGGCGCCTGGGTGTGCATCGAAGTTGCAGATACAGGCGCGGGGATAGCACCGGAGAACATCAAGAAAATCTTCGATCCTTTTTTCACCACCAAGCCCGTCGGCCAAGGTACCGGGCTAGGCCTGTCCCTTTCCTATGGCATTGTAAAGa < 2:55861/150‑1 (MQ=255) ggCGCCTGGGTGTGCATCGAAGTTGCAGATACAGGCGCGGGGATAGCACCGGAGAACATCAAGAAAATCTTCGATCCTTTTTTcac > 1:60628/1‑86 (MQ=255) ggCGCCTGGGTGTGCATCGAAGTTGCAGATACAGGCGCGGGGATAGCACCGGAGAACATCAAGAAAATCTTCGATCCTTTTTTcac < 2:60628/86‑1 (MQ=255) | AAGCCGACCTGGTAAAACGGTACCAGCCGTTACCCCCTGTCGAATGCCTGCCGTCGCAGATCAACCAAGTGATAATGAACCTGGTGCTCAACGCCGCCCAAGCCATGGGCCCCGAGCGGGGCACCATCACATTGGGCACTGCTCATCAAGGCGCCTGGGTGTGCATCGAAGTTGCAGATACAGGCGCGGGGATAGCACCGGAGAACATCAAGAAAATCTTCGATCCTTTTTTCACCACCAAGCCCGTCGGCCAAGGTACCGGGCTAGGCCTGTCCCTTTCCTATGGCATTGTAAAGA > NC_002947/3350562‑3350858 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |