| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,630,695 | T→A | 26.7% | W425R (TGG→AGG) | PP_3200 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,630,695 | 0 | T | A | 26.7% | 20.4 / 7.2 | 15 | W425R (TGG→AGG) | PP_3200 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (8/3); new base A (2/2); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01 | |||||||||||
GTTGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCTCGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGACGCCACCAGCCTGCGCCTGGTGTT > NC_002947/3630568‑3630843 | gTTGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCAGGCTGGCAAGCCAGGa < 1:413/144‑1 (MQ=255)gTTGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCAGGCTGGCAAGCCAGGa > 2:413/1‑144 (MQ=255) ttGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCtt > 1:462146/1‑132 (MQ=255) ttGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCtt < 2:462146/132‑1 (MQ=255) tGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCTCGGCGAAATCGCCTGGAACCggg < 1:251224/150‑1 (MQ=255) cGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCTCGGCGAAATCGCCTGGAACCGGGtgat > 1:2372/1‑150 (MQ=255) gggCGAGGCGATCGACAATGACGATAACNNGGTTTATGCCACCGNCNGNACGCTGCACGCCAACGTCTCCTGGCTTGCCGGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCTCGGCGAAATCGGCTGGAAGCGGGttgtgtg > 1:320860/1‑144 (MQ=255) gACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCTCGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAAc > 1:42946/1‑150 (MQ=255) gACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCTCGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGAt > 1:336566/1‑150 (MQ=255) aaggTTTATGCCACCGGCCGCACGTTGAACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCTCGGCGAAATCGCATGGGACCGGGTGATGAGGGTGAGCAAGAACGCCGATGCCGTTGAc > 2:469835/3‑150 (MQ=255) gttcggctcCAGGCTGGCAAGCCAGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCTCGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGACGcca > 1:5749/7‑140 (MQ=255) gttcggctcCAGGCTGGCAAGCCAGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCTCGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGACGcca < 2:5749/134‑1 (MQ=255) aCGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCTCGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAg > 1:221694/1‑98 (MQ=255) aCGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCTCGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAg < 2:221694/98‑1 (MQ=255) cTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCTCGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGCCCGTGACGCCACCAGCCTGCGCCTGGTGtt > 1:87460/1‑150 (MQ=255) | GTTGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCTCGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGACGCCACCAGCCTGCGCCTGGTGTT > NC_002947/3630568‑3630843 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |