Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	181,818	G→A	18.2%	intergenic (+32/‑74)	PP_r01 → / → PP_5SB	5S ribosomal RNA/5S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	181,818	0	G	A	18.2%	65.1 / 9.2	33	intergenic (+32/‑74)	PP_r01/PP_5SB	5S ribosomal RNA/5S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (15/12);  new base A (4/2);  total (19/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

TTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCC  >  NC_002947/181687‑181962                                                                                                                                    |                                                                                                                                                 ttGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCATATCTGCACATGCAGGTAGGGGTTTTGTCTTTaa                                                                                                                                <  1:204448/150‑1 (MQ=17)               tcccatcccGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACaga                                                                                                                  <  2:126941/150‑1 (MQ=40)                     cccGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCt                                                                                                                                    <  2:489278/126‑1 (MQ=33)                     cccGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCt                                                                                                                                    >  1:489278/1‑126 (MQ=33)                         aaCTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTCTCTGCACACGCAGGTAGGGGTTTTTTCTGTAAGTAGGAACTACAGAGATTCGCAgg                                                                                                        <  1:62132/150‑1 (MQ=255)                              agtagtGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGt                                                                                                   >  2:46000/1‑150 (MQ=255)                                   tGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAg                                                                                              <  2:393935/150‑1 (MQ=255)                                     aaaCGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAgaga                                                                                                                <  1:456248/130‑1 (MQ=255)                                     aaaCGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAgaga                                                                                                                >  2:456248/1‑130 (MQ=255)                                      aaCGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGa                                                                                           >  1:449753/1‑150 (MQ=255)                                         gATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAGAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACgg                                                                                        <  2:206425/150‑1 (MQ=255)                                                    gATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGcacaca                                                                             >  2:390508/1‑150 (MQ=255)                                                     aTGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTaa                                                                                                                                <  1:310716/98‑1 (MQ=34)                                                     aTGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTaa                                                                                                                                >  2:310716/1‑98 (MQ=34)                                                                gggTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCGATCTGCACATGCAGGGAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGCGCCAGCACACAGAATTTCTTgac                                                                 >  2:375989/1‑150 (MQ=255)                                                                             tgAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCAt                                                    >  1:44596/1‑150 (MQ=255)                                                                              gagaGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCAtt                                                   <  1:390508/150‑1 (MQ=255)                                                                                          tCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGa                                       >  2:316871/1‑150 (MQ=255)                                                                                            gTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGatc                                     >  1:211269/1‑150 (MQ=255)                                                                                                  aTTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTgacga                                                               >  2:484270/1‑118 (MQ=255)                                                                                                  aTTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTgacga                                                               <  1:484270/118‑1 (MQ=255)                                                                                                     ttaaagacAAAACCCCTACCTGCATGTGCAGATAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACCGGCCACCATACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGa                            >  2:67305/8‑150 (MQ=25)                                                                                                       tttCGCAAAACCACTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAAc                          >  1:40716/1‑150 (MQ=255)                                                                                                           acAAAACCCCTACCTGCATGTGCAGATAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGaa                                                                          <  2:324029/97‑1 (MQ=37)                                                                                                           acAAAACCCCTACCTGCATGTGCAGATAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGaa                                                                          >  1:324029/2‑98 (MQ=37)                                                                                                           acAAAACCCCTACCTGCATGTGCAGATAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGa                                                <  2:76012/123‑1 (MQ=38)                                                                                                           acAAAACCCCTACCTGCATGTGCAGATAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGa                                                >  1:76012/2‑124 (MQ=38)                                                                                                              aaaCCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTcagcag                   >  2:170165/1‑150 (MQ=255)                                                                                                                aCCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTg                 <  1:46000/150‑1 (MQ=255)                                                                                                                  cccTACCTGCATGTGCAGATAGGGGTTTTGTCTTTAAGTAGGAAATACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGaa               >  2:242618/1‑150 (MQ=37)                                                                                                                    cTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAAc             >  2:7482/1‑150 (MQ=255)                                                                                                                              aTGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGc   >  2:196668/1‑150 (MQ=255)                                                                                                                               tGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGcc  <  2:44596/150‑1 (MQ=255)                                                                                                                                    |                                                                                                                                                 TTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCC  >  NC_002947/181687‑181962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑