Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,130,031 A→T 13.8% L474Q (CTG→CAG)  recQ ← ATP‑dependent DNA 3'‑5' helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,130,0310AT13.8% 73.9 / 3.6 28L474Q (CTG→CAG) recQATP‑dependent DNA 3'‑5' helicase
Reads supporting (aligned to +/- strand):  ref base A (7/17);  new base T (2/2);  total (9/19)
Fisher's exact test for biased strand distribution p-value = 5.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGCTGCCGCCGCTGGACGACGAGGTTTTGGCCGTGGTCTGCGGTTTGAGATCACGGCGCAGTTGCAAGGTCACCTCGCCGCGCAGCAGCGGCCGGCAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAGCCTTTGCCAACGCCGAATACCGACAGCTTCTCGTGGCCGAAATTGCGCACCTTCTCGGTGTCCTTGCCCAACAGCACATCGACCAGGTGG  >  NC_002947/5129883‑5130174
                                                                                                                                                    |                                                                                                                                               
cGGGCTGCCGCCGCTGGACGACGAGGTTTTGGCCGTGGTCTGCGGTTTGAGATCACGGCGCAGTTGCAAGGTCACCTCGCCGCGCAGCAGCGGCCGGCAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAg                                                                                                                                                <  2:284606/150‑1 (MQ=255)
                gacgacGAGGTTTTGGCCGTGGTCTGCGGTTTGAGATCACGGCGCAGTTGCAAGGTCACCTCGCCGCGCAGCAGCGGCCGGCAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAAc                                                                                                                                <  2:295649/150‑1 (MQ=255)
                     cgaGGTTTTGGCCGTGGTCTGCGGTTTGAGATCACGGCGCAGTTGCAAGGTCACCTCGCCGCGCAGCAGCGGCCGGCAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACg                                                                                                                           <  2:148698/150‑1 (MQ=255)
                        ggTTTTGGCCGTGGTCTGCGGTTTGAGATCACGGCGCAGTTGCAAGGTCACCTCGCCGCGCAGCAGCGGCCGGCAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGaa                                                                                                                        <  1:46619/150‑1 (MQ=255)
                                 cGTGGTCTGCGGTTTGAGATCACGGCGCAGTTGCAAGGTCACCTCGCCGCGCAGCAGCGGCCGGCAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGAACAGTGAgcg                                                                                                               >  2:233115/1‑150 (MQ=255)
                                                    tCACGGCGCAGTTGCAAGGTCACCTCGCCGCGCAGCAGCGGCCGGCAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAg                                                                                            >  2:420391/1‑150 (MQ=255)
                                                         gcgcAGTTGCAAGGTCCCGGCGCCGCGCAGCAGCGGCCGGCAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAGCCttt                                                                                       <  1:271274/150‑1 (MQ=255)
                                                         gcgcAGTTGCAAGGTCACCTCGCCGCGCAGCAGCGGCCGGCAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAGCCttt                                                                                       <  1:46958/150‑1 (MQ=255)
                                                          cgcAGTTGCAAGGTCACCTCGCCGCGCAGCAGCGGCCGGCAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAGCCTTTg                                                                                      <  2:361382/150‑1 (MQ=255)
                                                              gTTGCAAGGTCACCTCGCCGCGCAGCAGCGGCCGGCAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAGCCTTTGCCaa                                                                                  <  2:478850/150‑1 (MQ=255)
                                                                 gCAAGGTCACCTCGCCGCGCAGCAGCGGCCGGCAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAGCCTTTGCCAACGc                                                                               >  1:18664/1‑150 (MQ=255)
                                                                     ggTCACCTCGCCGCGCAGCAGCGGCCGGCAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAGCCTTTGCCAACGCCGaa                                                                           <  2:466510/150‑1 (MQ=255)
                                                                     ggTCACCTCGCCGCGCAGCAGCGGCCGGCAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAGCCTTTGCCAACGCCGaa                                                                           <  1:420391/150‑1 (MQ=255)
                                                                        cACCTCGCCGCGCAGCAGCGGCCGGCAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAGCCTTTGCCAACGCCGAATAc                                                                        <  2:18664/150‑1 (MQ=255)
                                                                            tCGCCGCGCAGCAGCGGCCGGCAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAGCCTTTGCCAACGCCGAATACCGAc                                                                    >  2:9708/1‑150 (MQ=255)
                                                                                    cagcagCGGCCGGCAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAGCCTTTg                                                                                      <  1:71985/124‑1 (MQ=255)
                                                                                    cagcagCGGCCGGCAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAGCCTTTg                                                                                      >  2:71985/1‑124 (MQ=255)
                                                                                    cagcagCGGCCGGCAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAGCCTTTGCCAACGCCGAATACCGACAGCTtctc                                                            <  2:334869/150‑1 (MQ=255)
                                                                                        agcGGCCGGCAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAGCCTTTGCCAACGCCGAATACCGACAGCTTCTCGTgg                                                        <  1:9708/150‑1 (MQ=255)
                                                                                                gcAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAGCCTTTGCCAACGCCGAATACCGACAGCTTCTCGTGGCCGAAAtt                                                <  2:90678/150‑1 (MQ=255)
                                                                                                           aCAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAGCCTTTGCCAACGCCGAATACCGACAGCTTCTCGTGGCCGAAATTgcg                                             <  2:108001/142‑1 (MQ=255)
                                                                                                           aCAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAGCCTTTGCCAACGCCGAATACCGACAGCTTCTCGTGGCCGAAATTgcg                                             >  1:108001/1‑142 (MQ=255)
                                                                                                                         ccgGAGACTTCCAGGTCGAGGTGGACCAGGCCGCGCGCCACCAACTGACGCTACTGTGAGCGCCACTCGACCTCGGCTCAGCCTTTCCCAACGCCGAATACCTACAGCCTCTCGTGGCCGACCTTGCGCACCTTCTCGGTGTCCTTGccc                       >  2:31725/1‑150 (MQ=255)
                                                                                                                            tAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAGCCTTTGCCAACGCCGAATACCGACAGCTTCTCGTGGCCGAAATTGCGCACCTTCTCGGTGTCCTTGCCCAAc                    <  2:358006/150‑1 (MQ=255)
                                                                                                                                   cctggTCGATATCGACCTGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCAGCGAACTTCGGCCAAGACTTTTCCATCGCCGAATACCGACAGCTTCTCGTGGCCGAAATTGCGCACCTTCTCGGTGTCCTTGCCCAACAGCACAt             >  1:184381/4‑150 (MQ=255)
                                                                                                                                   cctggTCGATATCGACCTGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAGCCTTTGCCAACGCCGAATACCGACAGCTTCTCGTGGCCGAAATTGCGCACCTTCTCGGTGTCCTTGCCCAACAGCACAt             >  1:156975/4‑150 (MQ=255)
                                                                                                                                              tCGACCTGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAGCCTTTGCCAACGCCGAATACCGACAGCTTCTCGTGGCCGAAATTGCGCACCTTCTCGGTGTCCTTGCCCAACAGCACATCGACCAGGTgg  <  2:156975/150‑1 (MQ=255)
                                                                                                                                              tCGACCTGGCCGCGCGCCACCAACTGACGCAACAGTGAGCGCCACTCGACCTCGGCCAAGCCTTTGCCAACGCCGAATACCGACAGCTTCTCGTGGCCGAAATTGCGCACCTTCTCGGTGTCCTTGCCCAACAGCACATCGACCAGGTgg  <  2:184381/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CGGGCTGCCGCCGCTGGACGACGAGGTTTTGGCCGTGGTCTGCGGTTTGAGATCACGGCGCAGTTGCAAGGTCACCTCGCCGCGCAGCAGCGGCCGGCAGCTGTCGGACAGGCGCAGGCCGCCGTAGCCTTCCAGGTCGATATCGACCAGGCCGCGCGCCACCAACTGACGGAACAGTGAGCGCCACTCGACCTCGGCCAAGCCTTTGCCAACGCCGAATACCGACAGCTTCTCGTGGCCGAAATTGCGCACCTTCTCGGTGTCCTTGCCCAACAGCACATCGACCAGGTGG  >  NC_002947/5129883‑5130174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑