| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,569,322 | G→A | 15.4% | L44L (CTG→TTG) | tsaE ← | ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,569,322 | 0 | G | A | 15.4% | 56.0 / 4.5 | 26 | L44L (CTG→TTG) | tsaE | ATPase |
| Reads supporting (aligned to +/- strand): ref base G (12/10); new base A (2/2); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
TCCGGGTCGACCAGGCGATACAGGTCGAAGTGAAAGGCTCGGACCTCGCCGATTTCATAGGGTTCGACCACGGTGAACGTCGGGCTTTTCACAGCACCAGTATGGCCCAGACCGCGGATCAGGCCACGCGACAGGGTCGTTTTACCCGCGCCCAGGTCGCCCTCGAGGAAAATCACGCCGCGACCGCCGGTCACCTCAGCCAGTGCTGCACCGAATTTGACCGTGGCCTCTTCATCAGCCAGAAACAGATTTAAGCCAGACACGCAGAATGC > NC_002947/5569189‑5569460 | tccGGGTCGACCAGGCGATACAGGTCGAAGTGAAAGGCTCGGACCTCGCCGATTTCATAGGGTTCGACCACGGTGAACGTCGGGCTTTTCACAGCACCAGTATGGCCCAGACCGCGGATCAGGCCACGCGACAGGGTCGTTTTACCcgcg > 1:30955/1‑150 (MQ=255) aCCAGGCGATACAGGTCGAAGTGAAAGGCTCGGACCTCGCCGATTTCATAGGGTTCGACCACGGTGAACGTCGGGCTTTTCACAGCACCAGTATGGCCCAGACCGCGGATCAGGCCACGCGACAGGGTCGTTTTACCCGCGCCCAGGTCg > 2:119919/1‑150 (MQ=255) ggCGATACAGGTCGAAGTGAAAGGCTCGGACCTCGCCGATTTCATAGGGTTCGACCACGGTGAACGTCGGGCTTTTCACAGCACCAGTATGGCCCAGACCGCGGATCAGGCCACGCGACAGGGTCGTTGTACCCGCGCCCAGGTCGCCCt > 2:253680/1‑150 (MQ=255) aGGTCGAAGTGAAAGGCTCGGACCTCGCCGATTTCATAGGGTTCGACCACGGTGAACGTCGGGCTTTTCACAGCACCAGTATGGCCCAGACCGCGGATCAGGCCACGCGACAGGGTCGTTTTACCCGCGCCCAGGTCGCCCTCGAGGaaa > 1:199727/1‑150 (MQ=255) ggACCTCGCCGATTTCATAGGGTTCGACCACGGTGAACGTCGGGCTTTTCACAGCACCAGTATGGCCCAGACCGCGGATCAGGCCACGCGACAGGGTCGTTTTACCCGCGCCCAGGTCGCCCTCGAGGAAAATCACGCCGCGACCGCCgg > 2:112082/1‑150 (MQ=255) gCCGATTTCATAGGGTTCGACCACGGTGAACGTCGGGCGTTGCACAGCACCAGTATGGGCGAGACGGCGGATCAGGCGACGCGACAGGGTCGTTTTACCCGCGCCCAGGTCGACCTCGAGGAAAATCACGCCGCGACCGCCGGTCACCTc > 1:10949/1‑150 (MQ=255) tCATAGGGTTCGACCACGGTGAACGTCGGGCTTTTCACAGCACCAGTATGGCCCAGACCGCGGATCAGGCCACGCGACAGGGTCGTTTTACCCGCGCCCAGGTCGCCCTCGAGGAAAATCACGCCGCGACCGCCGGTCACCTCAGCCAGt > 1:426742/1‑150 (MQ=255) gACCACGGTGAACGTCGGGCTTTTCACAGCACCAGTATGGCCCAGACCGCGGATCAGGCCACGCGACAGGGTCGTTTTACCCGCGCCCAGGTCGCCCTCGAGGAAAATCACGCCGCGACCGCCGGTCACCTCAGCCAGTGCTGCACCGaa < 2:30955/150‑1 (MQ=255) cGTCGGGCTTTTCACAGCACCAGTATGGCCCAGACCGCGGATCAGGCCACGCGACAGGGTCGTTTTACCCGCGCCCAGGTCGCCCTCGAGGAAAATCACGCCGCGACCGCCGGTCACCTCAGCCAGTGCTGCACCGAATTTGACCGTGGc < 1:360578/150‑1 (MQ=255) gCTTTTCACAGCACCAGTATGGCCCAGACCGCGGATCAGGCCACGCGACAGGGTCGTTTTACCCGCGCCCAGGTCGCCCTCGAGGAAAATCACGCCGCGACCGCCGGTCACCTCAGCCAGTGCTGCACCGAATTTGACCGTGGCCTCTtc > 1:481435/1‑150 (MQ=255) ttttCACAACACCAGTATGGCCCAGCCCGCGGATCAGGCCACGCGACAGGGTCGTTTTACCCGCGCCCAGGTCGCCCTCGAGGAAAATCACGCCGCGACCGCCGGTCAc < 2:117833/109‑1 (MQ=255) ttttCACAACACCAGTATGGCCCAGACCGCGGATCAGGCCACGCGACAGGGTCGTTTTACCCGCGCCCAGGTCGCCCTCGAGGAAAATCACGCCGCGACCGCCGGTCAc > 1:117833/1‑109 (MQ=255) ttCACAGCACCAGTATGGCCCAGACCGCGGATCAGGCCACGCGACAGGGTCGTTTTACCCGCGCCCAGGTCGCCCTCGAGGAAAATCACGCCGCGACCGCCGGTCACCTCAGCCAGTGCTGCACCGAATTTGACCGTGGCCTCTtcatca < 2:481435/150‑1 (MQ=255) aCCAGTATGGCCCACACCGCGGATCAGGCCACGCGACAGGGTCGTTTTACCCGCGCCCAGTTCGCCCTCGAGGAAAATCACGCCGCGACCGCCGGTCACCTCAGCCAGTGCTGCACCGAATTTGACCGTGGCCTCTTCATCAGCCAGaaa < 2:10949/150‑1 (MQ=255) ccAGTATGGCCCAGACCGCGGATCAGGCCACGCGACAAGGTCGTTTTAccc < 1:19167/51‑1 (MQ=255) ccAGTATGGCCCAGACCGCGGATCAGGCCACGCGACAAGGTCGTTTTAccc > 2:19106/1‑51 (MQ=255) ccAGTATGGCCCAGACCGCGGATCAGGCCACGCGACAAGGTCGTTTTAccc > 2:19167/1‑51 (MQ=255) ccAGTATGGCCCAGACCGCGGATCAGGCCACGCGACAAGGTCGTTTTAccc < 1:19106/51‑1 (MQ=255) cAGTATGGCCCAGACCGCGGATCAGGCCACGCGACAGGGTCGTTTTACCCGCGCCCAGGTCGCCCTCGAGGAAAATCACGCCGCGACCGCCGGTCACCTCAGCCAGTGCTGCACCGAATTTGACCGTGGCCTCTTCATCAGCCAGAAACa < 2:199727/150‑1 (MQ=255) ggCCCAGACCGCGGATCAGGCCACGCGACAGGGTCGTTTTACCCGCGCCCAGGTCGCCCTCGAGGAAAATCACGCCGCGACCGCCGGTCACCTCAGCCAGTGCTGCACCGAATTTGACCGTGGCCTATTCATCAGGCAGAATCAGATTTa < 2:426742/150‑1 (MQ=255) gCCCAGACCGCGGATCAGGCCACGCGACAGGGTCGTTTTACCCGCGCCCAGGTCGCCCTCGAGGAAAATCACGCCGCGACCGCCGGTCACCTCAGCCAGTGCTGCACCGAATTTGACCGTGGCCTCTTCATCAGCCAGAAACAGATTTaa < 1:119919/150‑1 (MQ=255) cccAGACCGCGGATCAGGCCACGCGACAGGGTCGTTTTACCCGCGCCCAGGTCGCCCTCGAGGAAAATCACGCCGCGACCGCCGGTCACCTCAGCCAGTGCTGCACCGAATTTGACCGTGGCCTCTTCATCAGCCAGAAACAGATTTAAg > 2:453237/1‑150 (MQ=255) ccAGACCGCGGATCAGGCCACGCGACAGGGTCGTTTTACCCGCGCCCAGGTCGCCCTCGAGGAAAATCACGCCGCGACCGCCGGTCACCTCAGCCAGTGCTGCACCGAATTTGACCGTGGCCTCTTCATCAGCCAGAAACAGATTTAAGc < 1:41619/150‑1 (MQ=255) ccAGACCGCGGATCAGGCCACGCGACAGGGTCGTTTTACCCGCGCCCAGGTCGCCCTCGAGGAAAATCACGCCGCGACCGCCGGTCACCTCAGCCAGTGCTGCACCGAATTTGACCGTGGCCTCTTCATCAGCCAGAAACAGATTTAAGc > 2:41619/1‑150 (MQ=255) gcgGATCAGGCCACGCGACAGGGTCGTCTTACCCGCGCCCAGGTCGCCCTCGAGGAAAATCACGCCGCGACCGCCGGTCACCTCAGCCAGTGCTGCTCAAAATTTGACCGTGGCCTCTTCATCAGCCAGAAACAGATTTAAGCCAGacac < 1:258861/150‑1 (MQ=255) gcaaCGCGACAGGGTCGTTTTACCCGCGCCCAGGTCGCCCTCGAGGAAAATCACGCCGCGACCGCCGGTCACCTCAGCCAGTGCTGCACCGAATTTGACCGTGACCTCTTCATCAGCCAGAAACAGATTTAAGCCAGACACGCAGAATGc > 2:37140/4‑150 (MQ=255) | TCCGGGTCGACCAGGCGATACAGGTCGAAGTGAAAGGCTCGGACCTCGCCGATTTCATAGGGTTCGACCACGGTGAACGTCGGGCTTTTCACAGCACCAGTATGGCCCAGACCGCGGATCAGGCCACGCGACAGGGTCGTTTTACCCGCGCCCAGGTCGCCCTCGAGGAAAATCACGCCGCGACCGCCGGTCACCTCAGCCAGTGCTGCACCGAATTTGACCGTGGCCTCTTCATCAGCCAGAAACAGATTTAAGCCAGACACGCAGAATGC > NC_002947/5569189‑5569460 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |